FastQCFastQC Report
Tue 10 May 2022
EGAF00003676844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6944
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT851.224078341013825No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA570.8208525345622119No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA470.6768433179723502No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT370.5328341013824885No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA360.5184331797235023No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT300.4320276497695853No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG290.4176267281105991No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT280.4032258064516129No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT270.3888248847926267No Hit
ATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAG260.3744239631336405No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG250.36002304147465436No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT240.3456221198156682No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG240.3456221198156682No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA230.33122119815668205No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT220.31682027649769584No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA210.3024193548387097No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG200.28801843317972353No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT200.28801843317972353No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG190.2736175115207373No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT190.2736175115207373No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC190.2736175115207373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.2736175115207373No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG180.25921658986175117No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC180.25921658986175117No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG180.25921658986175117No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT180.25921658986175117No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG180.25921658986175117No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC180.25921658986175117No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC170.24481566820276496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.24481566820276496No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA160.2304147465437788No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG160.2304147465437788No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG160.2304147465437788No Hit
GTACATGGGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACA160.2304147465437788No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC160.2304147465437788No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT150.21601382488479265No Hit
GTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGC150.21601382488479265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.21601382488479265No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC150.21601382488479265No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT150.21601382488479265No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC140.20161290322580644No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT140.20161290322580644No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG140.20161290322580644No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG130.18721198156682026No Hit
TGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGG130.18721198156682026No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA130.18721198156682026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130.18721198156682026No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT130.18721198156682026No Hit
CCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTC130.18721198156682026No Hit
GTATCAACGCAGAGTACATGGGACGAGTCCTACACTATCTTGGATATGAT130.18721198156682026No Hit
GCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTT130.18721198156682026No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC130.18721198156682026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG130.18721198156682026No Hit
ATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTT120.1728110599078341No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA120.1728110599078341No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG120.1728110599078341No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA120.1728110599078341No Hit
CTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGC120.1728110599078341No Hit
ACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAG120.1728110599078341No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG120.1728110599078341No Hit
GCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCT120.1728110599078341No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC120.1728110599078341No Hit
ATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGAC110.15841013824884792No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA110.15841013824884792No Hit
GTTCACTATCGGTCGATGATGAGTATTTAGCCTTGGAGGATGGTCCCCCC110.15841013824884792No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG110.15841013824884792No Hit
GGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGC110.15841013824884792No Hit
TAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTA110.15841013824884792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG110.15841013824884792No Hit
CTGAATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGT110.15841013824884792No Hit
CTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGG100.14400921658986177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG100.14400921658986177No Hit
ATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAGCT100.14400921658986177No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT100.14400921658986177No Hit
AGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCG100.14400921658986177No Hit
CAACAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC100.14400921658986177No Hit
CCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGAT100.14400921658986177No Hit
CAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTT100.14400921658986177No Hit
AACATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCC100.14400921658986177No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG100.14400921658986177No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA100.14400921658986177No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG100.14400921658986177No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT100.14400921658986177No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA100.14400921658986177No Hit
GGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTT100.14400921658986177No Hit
CTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAA100.14400921658986177No Hit
CGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCAGTTTT100.14400921658986177No Hit
GCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGC100.14400921658986177No Hit
GGATTACACGTGTCCCGCCCTACTTGTCGTTAGCTCAGTACCACACGTCT90.12960829493087558No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT90.12960829493087558No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC90.12960829493087558No Hit
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT90.12960829493087558No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT90.12960829493087558No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.12960829493087558No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT90.12960829493087558No Hit
CTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAG90.12960829493087558No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT90.12960829493087558No Hit
GATGTTTGGTTAAAGAACAGTCGCAGTTTTCCTCAAATCCCGCCACGAAA90.12960829493087558No Hit
GCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGT90.12960829493087558No Hit
CCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTA90.12960829493087558No Hit
GTACAACACATTGTCCCGTTCCCTCACTCCGGCGCCACCCTGCGACTGCT90.12960829493087558No Hit
GTACATGGGAAGGTATCGCACACTGAATACATAGGTGTGCGAGGCGAACC90.12960829493087558No Hit
CATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAAT90.12960829493087558No Hit
CAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCG80.1152073732718894No Hit
ATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGA80.1152073732718894No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC80.1152073732718894No Hit
GTACATGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCC80.1152073732718894No Hit
AGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGG80.1152073732718894No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC80.1152073732718894No Hit
CTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGC80.1152073732718894No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA80.1152073732718894No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC80.1152073732718894No Hit
GTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACG80.1152073732718894No Hit
GTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTT80.1152073732718894No Hit
GGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTC80.1152073732718894No Hit
GTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGT80.1152073732718894No Hit
TTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGTGA80.1152073732718894No Hit
GCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGT80.1152073732718894No Hit
GCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGT80.1152073732718894No Hit
GAATACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAG80.1152073732718894No Hit
GATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCAC80.1152073732718894No Hit
GTATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA80.1152073732718894No Hit
CTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTG80.1152073732718894No Hit
CGCAAGGAGCGCCTGCTTCTTCGCCGCGACTCGATCGCCGAGCTCCTCCG70.10080645161290322No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA70.10080645161290322No Hit
GACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAG70.10080645161290322No Hit
ATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGT70.10080645161290322No Hit
GTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTA70.10080645161290322No Hit
GATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGG70.10080645161290322No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC70.10080645161290322No Hit
CGCTTACACACCGGACGCTTGGATCAGTGGGAAGTGCTCACGCGCGGAGC70.10080645161290322No Hit
CTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTC70.10080645161290322No Hit
CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC70.10080645161290322No Hit
AGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCT70.10080645161290322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG70.10080645161290322No Hit
CTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGC70.10080645161290322No Hit
TCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGG70.10080645161290322No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC70.10080645161290322No Hit
ATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTG70.10080645161290322No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC70.10080645161290322No Hit
ACATAGGTGTGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAGCTCG70.10080645161290322No Hit
GATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGC70.10080645161290322No Hit
GTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTAT70.10080645161290322No Hit
GACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTT70.10080645161290322No Hit
TTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATC70.10080645161290322No Hit
ATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTG70.10080645161290322No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA70.10080645161290322No Hit
TAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATACT70.10080645161290322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)