FastQCFastQC Report
Tue 10 May 2022
EGAF00003676848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169061
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT57690.493472966765635No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC41440.35447252110882155No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC41030.35096543294148036No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT38130.326159199562726No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA32880.2812513632736016No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG30690.2625183801358526No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG28890.24712140769386715No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT28380.2427589321686379No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA28080.24019277009497364No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG28000.23950846020866318No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG25730.2200911671846037No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT25630.2192357798267156No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT25200.21555761418779687No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG24920.21316252958571025No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT24050.2057206595720839No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA24020.2054640433647175No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT23690.20264126508368682No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA22760.19468616265532765No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG22580.19314646541112912No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG22170.18963937724378796No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG22020.18835629620695585No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC21830.18673106022696848No Hit
GGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC21640.18510582424698113No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG21640.18510582424698113No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT21490.183822743210149No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC20580.17603871825336745No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG19970.17082085537025016No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC19570.1673993059386978No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG19230.16449098892187833No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA18870.16141159443348124No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC18590.1590165098313946No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG18510.15833219994508413No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC18320.15670696396509678No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT17630.150804791195669No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC17600.15054817498830259No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG17340.14832416785779357No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA17150.1466989318778062No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC17050.14584354451991813No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC16560.1416521464662665No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA16220.13874382944944705No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA15380.13155857564318713No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC15300.13087426575687666No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG14750.1261696352884922No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC14660.12539978666639293No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA14550.12445886057271605No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT14320.12249146964957347No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT14110.12069515619800848No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA14070.12035300125485325No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT13950.11932653642538756No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG13880.11872776527486589No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC13790.11795791665276663No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT13520.1156483707864688No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT13410.1147074446927919No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG13280.1135954411275374No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC13080.11188466641176124No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC12680.1084631169802089No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA12360.10572587743496704No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT12350.10564033869917824No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC12250.10478495134129014No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG12160.10401510271919087No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG12080.10333079283288041No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG11950.1022187892676259No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG11700.10008032087290568No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)