FastQCFastQC Report
Tue 10 May 2022
EGAF00003676970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335431
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA12590.3753379979787199No Hit
GTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG12310.3669905286034982No Hit
TGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAA11850.3532768289156339No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG11300.3368800140714484No Hit
GTATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCT10580.3154150928208782No Hit
GTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAGAAAAG8280.24684659438155687No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT7110.21196609734938035No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC7080.2110717256306066No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6640.19795427375525815No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT6530.19467491078642107No Hit
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTG6510.1940786629739052No Hit
TATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTC6190.1845386979736518No Hit
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT6110.18215370672358847No Hit
GCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAAATTAGCCCGA5780.17231561781707713No Hit
GTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCT5620.16754563531695044No Hit
GTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTG5580.16635313969191876No Hit
ACGTTTACCTGTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTG5530.16486252016062916No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5470.16307377672308163No Hit
GAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAAC5270.15711129859792328No Hit
AGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCC5240.1562169268791495No Hit
ACTTATAGAAAAGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTG5210.15532255516037574No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG5140.15323568781657032No Hit
CTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGC5120.1526394400040545No Hit
GGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTC5000.14906195312895948No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4990.14876382922270154No Hit
CATCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGG4940.14727320969141194No Hit
TAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTAT4940.14727320969141194No Hit
GTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAAT4890.14578259016012235No Hit
ACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCT4880.14548446625386444No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG4770.1422051032850273No Hit
ATTTGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTATTTC4720.14071448375373774No Hit
GAATGGTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAG4660.1389257403161902No Hit
CCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCT4660.1389257403161902No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT4630.13803136859741647No Hit
TCCTGGAAGAGAGCGCGGCCGCCACGCTGGTTTTGCATCTTCAGGAGACG4610.1374351207849006No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG4610.1374351207849006No Hit
GTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCC4570.13624262515986896No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG4470.13326138609728977No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA4410.13147264265974223No Hit
GCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTACT4320.12878952750342096No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA4280.12759703187838928No Hit
CATTATCACTGTCTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAG4140.12342329719077844No Hit
TGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCT4080.12163455375323093No Hit
CTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCTCT3990.11895143859690965No Hit
GCTTTATTTCCATTTGGTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGC3840.11447958000304086No Hit
CTCATGAGGAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAA3830.11418145609678294No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA3820.11388333219052502No Hit
CTCCGGGACCATCTTCTCGGCCATCTCCTGCTTCTGGGACCTGCCAGCAC3730.11120021703420375No Hit
TCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGGC3720.11090209312794583No Hit
AGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGT3720.11090209312794583No Hit
GTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTC3690.11000772140917207No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA3650.1088152257841404No Hit
GCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTATTACTTTGCAA3640.10851710187788248No Hit
GAATTATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTTGT3630.10821897797162457No Hit
CCTTAGTCGCCGCCATGACGACCGCGTCCACCTCGCAGGTGCGCCAGAAC3610.10762273015910873No Hit
ACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG3610.10762273015910873No Hit
GATGGGGGTCCGTGCGGGCAGAACCCAGGGCATGAAGATCCAAAAGGGCC3580.10672835844033497No Hit
CTCCTTAGTCGCCGCCATGACGACCGCGTCCACCTCGCAGGTGCGCCAGA3570.10643023453407706No Hit
TCAACAGCCTGGTCAATTTGTACCTGCAGGCCTCCTACACCTACCTCTCT3520.10493961500278746No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG3480.10374711937775578No Hit
TCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAATA3460.10315087156523996No Hit
CTCTCTGGGCTTCTATTTCGACCGCGATGATGTGGCTCTGGAAGGCGTGA3450.10285274765898203No Hit
ACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCTCTTAGTC3430.1022564998464662No Hit
GGCTACGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCT3370.10046775640891867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)