FastQCFastQC Report
Tue 10 May 2022
EGAF00003676971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331160
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAA13190.39829689576035754No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA11630.3511897572170552No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG11360.3430365986230221No Hit
GTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG10210.3083101823891774No Hit
GTATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCT9620.29049402101703103No Hit
GTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAGAAAAG8870.26784635825582803No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT7070.21349196762894068No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC7060.21318999879212466No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6350.1917502113781858No Hit
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTG6230.1881265853363933No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT6180.18661674115231308No Hit
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT5870.17725570721101583No Hit
ACTTATAGAAAAGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTG5680.17151829931151105No Hit
TATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTC5640.1703104239642469No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG5440.16427104722792607No Hit
GCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAAATTAGCCCGA5370.1621572653702138No Hit
GGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTC5290.15974151467568548No Hit
GTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCT5250.1585336393284213No Hit
GTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTG5190.15672182630752507No Hit
ATTTGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTATTTC5120.15460804444981277No Hit
ACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCT5020.15158835608165236No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4970.15007851189757218No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4960.14977654306075613No Hit
TAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTAT4930.148870636550308No Hit
GTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAAT4820.14554897934533156No Hit
CATCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGG4810.14524701050851552No Hit
AGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCC4810.14524701050851552No Hit
GCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTACT4760.1437371663244353No Hit
GAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAAC4700.14192535330353906No Hit
ACGTTTACCTGTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTG4680.141321415629907No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT4460.1346781012199541No Hit
CCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCT4460.1346781012199541No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG4450.13437613238313806No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG4440.134074163546322No Hit
CATTATCACTGTCTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAG4440.134074163546322No Hit
CTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGC4430.13377219470950596No Hit
TCCTGGAAGAGAGCGCGGCCGCCACGCTGGTTTTGCATCTTCAGGAGACG4380.13226235052542576No Hit
TGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCT4300.12984659983089744No Hit
GAATGGTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAG4290.12954463099408142No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA4140.1250150984418408No Hit
GTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCC4110.12410919193139268No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA3920.1183717840318879No Hit
CTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCTCT3900.11776784635825584No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG3830.11565406450054355No Hit
AGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGT3800.11474815799009541No Hit
CTCCTTAGTCGCCGCCATGACGACCGCGTCCACCTCGCAGGTGCGCCAGA3680.11112453194830293No Hit
CTCATGAGGAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAA3680.11112453194830293No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA3670.11082256311148689No Hit
ACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG3640.10991665660103878No Hit
TCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGGC3610.10901075009059065No Hit
GAATTATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTTGT3570.1078028747433265No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG3570.1078028747433265No Hit
GCTTTATTTCCATTTGGTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGC3560.10750090590651044No Hit
GTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTC3470.10478318637516608No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT3440.10387727986471795No Hit
CCTTAGTCGCCGCCATGACGACCGCGTCCACCTCGCAGGTGCGCCAGAAC3430.10357531102790192No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG3400.10266940451745381No Hit
TCAACAGCCTGGTCAATTTGTACCTGCAGGCCTCCTACACCTACCTCTCT3390.10236743568063776No Hit
CTCTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCC3380.10206546684382173No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA3370.10176349800700567No Hit
GATGGGGGTCCGTGCGGGCAGAACCCAGGGCATGAAGATCCAAAAGGGCC3350.10115956033337359No Hit
GCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTATTACTTTGCAA3340.10085759149655756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)