FastQCFastQC Report
Tue 10 May 2022
EGAF00003676972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003676972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331160
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAA11640.35149172605387125No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA11450.34575431815436647No Hit
GTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG11040.3333735958449088No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG10760.32491846841405964No Hit
GTATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCT9610.29019205218021504No Hit
GTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAGAAAAG7660.2313081290010871No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT7420.22406087691750212No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC7120.2150018118130209No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6310.1905423360309216No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT6280.18963642952047347No Hit
CTACTGGAACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTG5970.18027539557917624No Hit
TATCAACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTC5820.17574586302693562No Hit
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT5610.16940451745379878No Hit
GCCTTGGTGACCAGGGAAGTCACCCCACGGCTATGGGGAAATTAGCCCGA5600.16910254861698273No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5600.16910254861698273No Hit
GGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTC5380.16245923420702982No Hit
GTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCT5350.1615533276965817No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG5280.15943954583886943No Hit
ACGTTTACCTGTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTG5280.15943954583886943No Hit
ACTTATAGAAAAGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTG5220.15762773281797318No Hit
GAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAAC5180.15641985747070902No Hit
ACGCAGAGTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCT5030.15189032491846843No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4970.15007851189757218No Hit
GTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAAT4960.14977654306075613No Hit
ATTTGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTATTTC4930.148870636550308No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA4810.14524701050851552No Hit
TCCTGGAAGAGAGCGCGGCCGCCACGCTGGTTTTGCATCTTCAGGAGACG4760.1437371663244353No Hit
CCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGTCACCCCACGGCT4730.1428312598139872No Hit
CTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGGGC4640.14011354028264283No Hit
CTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCTCT4600.13890566493537868No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG4590.13860369609856263No Hit
AGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCC4540.13709385191448242No Hit
GTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTG4490.13558400773040222No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG4470.13498007005677015No Hit
CATTATCACTGTCTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAG4470.13498007005677015No Hit
GAATGGTACAAATCAAAGAACTTAAGTGGATGTTTTGGTACAACTTATAG4430.13377219470950596No Hit
CATCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGG4420.13347022587268995No Hit
TGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCT4360.1316584128517937No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT4350.13135644401497765No Hit
TAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTAT4340.13105447517816163No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG4270.12894069332044933No Hit
GTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCC4060.12259934774731247No Hit
GCAATGGAGTGTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTACT4040.1219954100736804No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA4030.12169344123686436No Hit
AGGTAAAGGAAACCCCAACATGCATGCACTGCCTTGGTGACCAGGGAAGT4020.12139147240004833No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA4010.12108950356323227No Hit
CTCATGAGGAGAGGGAACATGCTGAGAAACTGATGAAGCTGCAGAACCAA3740.11293634496919917No Hit
GTCTCCCAGGGTGTGCTTGTCAAAGAGATATTCCGCCAAGCCAGATTCGG3710.11203043845875106No Hit
TCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGGC3670.11082256311148689No Hit
ACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG3650.11021862543785482No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA3610.10901075009059065No Hit
TCAACAGCCTGGTCAATTTGTACCTGCAGGCCTCCTACACCTACCTCTCT3500.10568909288561419No Hit
GAATTATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTTGT3500.10568909288561419No Hit
GCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCTATTACTTTGCAA3440.10387727986471795No Hit
TCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAATA3400.10266940451745381No Hit
GATGGGGGTCCGTGCGGGCAGAACCCAGGGCATGAAGATCCAAAAGGGCC3390.10236743568063776No Hit
CCTTAGTCGCCGCCATGACGACCGCGTCCACCTCGCAGGTGCGCCAGAAC3380.10206546684382173No Hit
CTCTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCC3360.10146152917018963No Hit
GTGCATTACATTTGGAAAAAAATGTGAATCAGTCACTACTGGAACTGCAC3360.10146152917018963No Hit
CTCTCTGGGCTTCTATTTCGACCGCGATGATGTGGCTCTGGAAGGCGTGA3330.10055562265974152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)