FastQCFastQC Report
Tue 10 May 2022
EGAF00003677174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245342
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT57002.323287492561404No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG35571.4498129142177043No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT24831.0120566393035029No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT20970.8547252406844323No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20380.830677177164937No Hit
GTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTC15100.6154673883803018No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA14720.5999788050965591No Hit
GAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA12430.5066397110971623No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10560.43041957756927063No Hit
TATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCT10530.42919679467844885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.4275664174906864No Hit
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT10400.42389806881822106No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC10140.41330061709776555No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT10050.4096322684253002No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT9510.38762217639050794No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG9290.3786551018578148No Hit
ACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCT9180.3741715645914683No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA9160.37335637599758703No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT8660.3529766611505572No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT7480.30488053411156674No Hit
GTACATGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT7200.29346789379723No Hit
GAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC7060.2877615736400616No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC6730.27431096184102194No Hit
GGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG6630.27023501887161594No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG6530.26615907590220994No Hit
TATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTG6250.25474643558787324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.25433884129093265No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG5970.24333379527353652No Hit
GTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC5820.23721988081942758No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG5740.23395912644390277No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA5370.2188781374571007No Hit
ACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTA5330.21724776026933829No Hit
CATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC5280.21520978878463531No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT4950.20175917698559565No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC4870.19849842261007083No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG4830.19686804542230846No Hit
ACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT4760.19401488534372427No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC4760.19401488534372427No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG4680.1907541309681995No Hit
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC4510.18382502792020933No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG4510.18382502792020933No Hit
GGTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGT4250.17322757619975382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4060.16548328455788247No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC4060.16548328455788247No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3790.15447823854048634No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG3790.15447823854048634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3680.14999470127413977No Hit
CCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG3620.14754913549249618No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT3580.1459187583047338No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG3570.1455111640077932No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG3510.14306559822614962No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT3510.14306559822614962No Hit
TCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC3460.14102762674144662No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG3450.14062003244450605No Hit
GGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTC3400.13858206095980305No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT3310.13491371228733767No Hit
ATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTG3310.13491371228733767No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC3300.13450611799039708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTAA3170.1292073921301693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTA3140.12798460923934754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT3040.12390866626994154No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2970.12105550619135738No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA2880.11738715751889199No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC2770.11290362025254543No Hit
GGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGT2740.11168083736172363No Hit
ACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC2720.11086564876784243No Hit
CTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTG2640.10760489439231766No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2620.10678970579843647No Hit
CACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCC2600.10597451720455527No Hit
CCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGT2570.10475173431373347No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC2560.10434414001679289No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG2490.1014909799382087No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG2460.1002681970473869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)