FastQCFastQC Report
Tue 10 May 2022
EGAF00003677242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444609
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG50861.1439264612277305No Hit
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC41130.9250824882087407No Hit
GTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT40860.9190097366450071No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT25470.5728628975121961No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA19750.44421053105087843No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT14360.32298041650079057No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA13930.3133089973437335No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT13870.31195949699623715No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC13600.3058867454325036No Hit
GTATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTC13410.30161332766543186No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT12660.28474457332172765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.2773223214104978No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12060.27124956984676424No Hit
GTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGT11940.26855056915177156No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT11660.26225290086345526No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT11300.2541558987784773No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG11170.25123198135890185No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT9580.21547022215024889No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9420.2118715545569253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG9400.21142172110775984No Hit
TATCAACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCT8870.19950113470487552No Hit
GTACATGGGCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA8750.19680213400988283No Hit
TATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTC8750.19680213400988283No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC8730.1963523005607174No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG8350.18780546502657391No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT7680.17273604447953145No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA7610.1711616274074524No Hit
GAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA7440.16733804308954608No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGT7420.16688820964038065No Hit
ACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC7190.161715124974978No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG7180.16149020825039528No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG7120.16014070790289894No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG6870.15451778978833086No Hit
GTACATGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC6740.15159387236875546No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC6580.1479952047754319No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC6580.1479952047754319No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG6530.14687062115251828No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC6480.14574603752960466No Hit
GGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG6310.14192245321169836No Hit
ACGCAGAGTACATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCT6210.13967328596587114No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG6120.13764903544462664No Hit
GAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCA6100.13719920199546118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.13562478492338212No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT5790.13022678353339676No Hit
ACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT5770.12977695008423132No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA5650.12707794938923864No Hit
CATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT5640.12685303266465592No Hit
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT5620.12640319921549045No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG5580.12550353231715958No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG5530.12437894869424596No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGA5460.1228045316221669No Hit
CATGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC5350.12033044765175695No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT5150.11583211316010247No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG4990.11223344556677892No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG4910.11043411177011711No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG4740.10661052745221081No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA4670.10503611038013175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.10346169330805269No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4580.10301185985888725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4580.10301185985888725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)