FastQCFastQC Report
Tue 10 May 2022
EGAF00003677701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540238
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG46850.8672103776483697No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG41070.7602204954112817No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC29560.5471662489495371No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG28570.5288409923033922No Hit
GTATATAGGCTGAGCAAGAGGTGGTAAGGTTGATCGGGGTTTATCGATTA21630.40037909217789197No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT20050.37113272298505473No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA19030.35225215553145095No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT18930.35040111950658787No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC18260.33799917814000496No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG17540.3246717187609905No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT16930.3133803990093255No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA16400.303569908077551No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC11900.2202732869587108No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG11360.21027769242444996No Hit
GTACATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATAT11240.20805644919461422No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG11220.20768624198964158No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC10930.20231823751753858No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC10600.19620981863549025No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT10090.1867695349086884No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA9990.18491849888382528No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC9950.18417808447388004No Hit
ACCTTACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCT9790.18121642683409905No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA9740.18029090882166748No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC9580.1773292511818865No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA9380.17362717913216025No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC9220.1706655214923793No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA9210.170480417889893No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG9160.16955489987746142No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG9130.16899958907000248No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC8360.1547466116785565No Hit
GTTCTGTAATCGATAAACCCCGATCAACCTTACCACCTCTTGCTCAGCCT8230.15234026484623445No Hit
GAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCC8180.15141474683380288No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA8030.14863819279650822No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG7970.14752757118159035No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC7680.1421595667094873No Hit
GGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAG7630.14123404869705575No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT7480.13845749465976107No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG7420.13734687304484322No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC7420.13734687304484322No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC7410.1371617694423569No Hit
ACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAG7320.13549583701998008No Hit
TAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACC7110.13160866136776755No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG7050.13049803975284968No Hit
CTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCT6980.12920231453544548No Hit
GCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCC6980.12920231453544548No Hit
AGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAAC6810.1260555532931782No Hit
GTGGTAAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAG6760.12513003528074662No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC6660.12327899925588352No Hit
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT6560.1214279632310204No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT6530.12087265242356146No Hit
ATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA6480.1199471344111299No Hit
CTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGA6390.1182812019887531No Hit
AGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAA6220.11513444074648582No Hit
TCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAAC6220.11513444074648582No Hit
GCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTC6130.11346850832410901No Hit
GTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGT5780.1069898822370881No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG5670.10495374260973866No Hit
CAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAG5670.10495374260973866No Hit
GTAATCGATAAACCCCGATCAACCTTACCACCTCTTGCTCAGCCTATATA5670.10495374260973866No Hit
TCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTAT5640.10439843180227973No Hit
TCATAAGGGCTATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCATTTCTT5620.10402822459730711No Hit
TCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTAC5550.10273249937990292No Hit
AGTTAAATGTCCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTA5410.10014104894509455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)