FastQCFastQC Report
Tue 10 May 2022
EGAF00003677702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540238
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG48780.9029353729282279No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG39910.7387484775228695No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG29510.5462407309371055No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC29500.5460556273346192No Hit
GTATATAGGCTGAGCAAGAGGTGGTAAGGTTGATCGGGGTTTATCGATTA21430.39667702012816575No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT19510.36113712845079393No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA19390.3589158852209582No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT18950.35077132671156047No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC18910.3500309123016152No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG16650.3081974981397088No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA16000.2961657639780986No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT15550.2878361018662145No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC11990.2219392193810876No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG11520.21323935006423095No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC11330.20972238161699103No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG10780.19954168348024387No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA10350.19158222857333249No Hit
GTACATGGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATAT10290.1904716069584146No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT10120.18732484571614733No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC9790.18121642683409905No Hit
ACCTTACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCT9780.18103132323161272No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG9580.1773292511818865No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC9430.17455269714459185No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA9250.17122083229983823No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC9120.16881448546751618No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA9020.16696344944265304No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC8970.1660379314302215No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA8960.1658528278277352No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG8740.1617805485730363No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA8520.15770826931833748No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC8490.15715295851087854No Hit
GAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCC8460.1565976477034196No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC8290.1534508864611523No Hit
GTTCTGTAATCGATAAACCCCGATCAACCTTACCACCTCTTGCTCAGCCT8200.1517849540387755No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG7950.14715736397661772No Hit
TAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACC7750.1434552919268915No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC7570.14012342708213787No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT7500.1388277018647337No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG7470.13827239105727476No Hit
GGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAG7350.13605114782743902No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC7340.1358660442249527No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG7160.1325341793801991No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC7130.13197886857274016No Hit
CTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGA7080.13105335056030862No Hit
ACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAG6800.1258704496906919No Hit
GTGGTAAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAG6620.12253858484593827No Hit
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT6580.12179817043599303No Hit
TCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAAC6430.11902161639869835No Hit
GCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCC6420.11883651279621203No Hit
AGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAAC6270.11605995875891738No Hit
CTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCT6150.11383871552908163No Hit
GCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTC6140.1136536119265953No Hit
GTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGT6120.1132834047216227No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT5970.11050685068432801No Hit
GTAATCGATAAACCCCGATCAACCTTACCACCTCTTGCTCAGCCTATATA5940.10995153987686909No Hit
TCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTAT5870.1086558146594649No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG5860.10847071105697859No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.10810050385200597No Hit
ATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA5830.10791540024951965No Hit
TCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTAC5800.10736008944206073No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA5800.10736008944206073No Hit
GGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG5630.10421332819979343No Hit
GCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAGGAGGGTGA5530.1023622921749303No Hit
AGTTAAATGTCCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTA5490.10162187776498507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)