FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003677731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGTGGATACTGCGCCGGCGTCGACCGAGCCGTCATCGCGGTGGAGAA211.11111111111111No Hit
GTGGCAATCAATCTTGATCAACATGGGACACATCCCTAGATGCTTCAATG15.555555555555555No Hit
CTAAACAAAATCAAACAAAAACAAATAACCAACACCAACAGACAAGTTCA15.555555555555555No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15.555555555555555Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCCGGCCCCACCTGTCCCAAGAGCCTTCCCACCTACACAGGTCAGAGG15.555555555555555No Hit
TTCCTGCCCAACGAAAATGCTTGCTACGGGGCGCAAGACCGCATGGAACG15.555555555555555No Hit
CGTCAGATGTGTATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA15.555555555555555No Hit
GGTAACAACACATACTAAAAAAATATTTATATTATAAAAATATAATAGCC15.555555555555555No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15.555555555555555No Hit
ATCCTGGATCCCGAAGATGACAAAGGCAGTTCCCTCCACTTTCTTCCCGT15.555555555555555No Hit
ACGCAGAGTACATGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC15.555555555555555No Hit
TTCCAGGGTAGTCAGGGCCTCCACCCCGCACTGGCCCAGAGCTGTCTCTT15.555555555555555No Hit
CCTCCGGTGAGCAGGGTCAGTGGAAGCCCCAACGGGAAAGGAAACGCCCC15.555555555555555No Hit
GATCTCGGTGGTCGCCGTCCCATGTACTCTGCGTTGATACCTGTCTCTTA15.555555555555555No Hit
CGTCAGATGTGTATAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGA15.555555555555555No Hit
GATCTCGGTGGTCGCCGTATCACCCATGTACTCTGCGTTGATACCTGTCT15.555555555555555Illumina Single End PCR Primer 1 (95% over 22bp)
GCGCCAAGGAGTGAGCCGAAGTTTCATCATGCGGAGATGTTGGATGGGGT15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)