FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003677732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCACGGTGCACCTTTGTCACGAGCGGACACGTCGCGTCGATCGCCTG211.11111111111111No Hit
CTCTGGGCCAGTGCGGGGTGGAGGCCCTGACTACCCTGGAACTGTCTCTT15.555555555555555No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCCCATGTA15.555555555555555No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTAACACAGCCCCCA15.555555555555555No Hit
GTGTGGTGGTGCACACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGG15.555555555555555No Hit
GCTCTGACCCACAGACTGCAAACCCAGCCTGCCTGCACAGGGTTCAAAAT15.555555555555555No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCACTCCCA15.555555555555555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATT15.555555555555555No Hit
CGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGGAA15.555555555555555Illumina Single End PCR Primer 1 (100% over 26bp)
GTTGGTACCAGATCGGAAGAGCGTCGTGTAGGAAAGAGTGTAGCTGTCTC15.555555555555555Illumina Single End PCR Primer 1 (96% over 26bp)
GTATCAACGCAGAGTACATGGGTGATACGGCGACCACCGAGATCCTGTCT15.555555555555555Illumina Single End PCR Primer 1 (95% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15.555555555555555Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAT15.555555555555555No Hit
GGACAAGCGAGCAGTCGACACGATTCGGGTGTTGGCCGCCGATGCGGTGG15.555555555555555No Hit
CTCATTCATCGACCTCCCCACCCCATCCAACATCTCCGCATGATGAAACT15.555555555555555No Hit
CCACAGCAGGTCTTCTCCACTGAGTTTGAGGTGAAGGAGTACGTGCTGCC15.555555555555555No Hit
GTATCAACGCAGAGTACATGGGACGGCGACCACCGAGATCCTGTCTCTTA15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)