FastQCFastQC Report
Tue 10 May 2022
EGAF00003677738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10930
Sequences flagged as poor quality0
Sequence length140
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC890.8142726440988106No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT610.5580969807868252No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA600.5489478499542544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.43000914913083255No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC420.384263494967978No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG410.3751143641354071No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT360.3293687099725526No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT340.31107044830741076No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA340.31107044830741076No Hit
GGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAG330.3019213174748399No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC330.3019213174748399No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT320.292772186642269No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG320.292772186642269No Hit
GTACATGGGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTG310.2836230558096981No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT300.2744739249771272No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA300.2744739249771272No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC280.2561756633119854No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC270.24702653247941445No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG270.24702653247941445No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG270.24702653247941445No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA270.24702653247941445No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC260.23787740164684354No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA260.23787740164684354No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC250.22872827081427266No Hit
TCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGG230.21043000914913085No Hit
GTACATGGGTCTGCGGATCAGTCTATCCTCGCTGATGCTCAGTGATACAC230.21043000914913085No Hit
ATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGA220.2012808783165599No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC220.2012808783165599No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG210.192131747483989No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT210.192131747483989No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT200.18298261665141813No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG200.18298261665141813No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA190.17383348581884722No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC190.17383348581884722No Hit
GTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAAAGAACAGTCGCA190.17383348581884722No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG190.17383348581884722No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT180.1646843549862763No Hit
GTACACAGATACGCGCTCAGCATAGCAGATGCACGTTGAGTCCACGGGAT180.1646843549862763No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG180.1646843549862763No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA180.1646843549862763No Hit
GCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCG180.1646843549862763No Hit
GTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACT180.1646843549862763No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT180.1646843549862763No Hit
ATATTTGGTCCGGGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCA170.15553522415370538No Hit
CTCTGGGACTATGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGG170.15553522415370538No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG170.15553522415370538No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA170.15553522415370538No Hit
CTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTG170.15553522415370538No Hit
GTCGCAATCCTGCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTT170.15553522415370538No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC170.15553522415370538No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT170.15553522415370538No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT170.15553522415370538No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA170.15553522415370538No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT160.1463860933211345No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG160.1463860933211345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1463860933211345No Hit
GTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGT160.1463860933211345No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC160.1463860933211345No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG160.1463860933211345No Hit
GTGATACACGCTGATGCACGTAGTGAGCATCCTGTGCTCGCATATACCGC160.1463860933211345No Hit
ACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTG160.1463860933211345No Hit
ACTTTACGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCC160.1463860933211345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1463860933211345No Hit
GTACATGGGAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAACGC150.1372369624885636No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT150.1372369624885636No Hit
GTCTAGCACTGTAGCTGGTGCATGTACTCGACTGCCGCTGCATCTACTAT150.1372369624885636No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC150.1372369624885636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150.1372369624885636No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA140.1280878316559927No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG140.1280878316559927No Hit
GTACATGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGC140.1280878316559927No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC140.1280878316559927No Hit
GCATGAAACTCCAAGGTCCGGGGGGGTCACTTATCCTGGGCTTCATCCAA140.1280878316559927No Hit
GTCTTATCGAGCACAGTGTCGGCTATGAGCGCACAGTCTCGTCATATAAC140.1280878316559927No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG140.1280878316559927No Hit
GTATATGAGCTACCGAGTCGTTCTGATAGATCCCGCGTCAGGCATATAAT140.1280878316559927No Hit
CGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTG140.1280878316559927No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA140.1280878316559927No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT140.1280878316559927No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG130.11893870082342177No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT130.11893870082342177No Hit
GTGTTATTGCAGCGTCTCGATTAGGGGGAAACCTTGTCACCGTCAGCTTA130.11893870082342177No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC130.11893870082342177No Hit
CTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTC130.11893870082342177No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT130.11893870082342177No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA130.11893870082342177No Hit
AACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGA130.11893870082342177No Hit
CGCATATACCGCTGCTGCACTGATATGAGCCAGTGCTGCTGCTCTCTACG130.11893870082342177No Hit
GAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTA130.11893870082342177No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC130.11893870082342177No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT130.11893870082342177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG130.11893870082342177No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA130.11893870082342177No Hit
CTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGAAT130.11893870082342177No Hit
GGTTATTATCGTCGCGGTTACCGTAATACTTCAGATCAGTTAAGTAGGGC130.11893870082342177No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG120.10978956999085088No Hit
CTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGATCCCTTCCG120.10978956999085088No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG120.10978956999085088No Hit
GCGTAATCAACGCCGCAACTTTACGTCGGATTAGCTCTTCTTTGATTAGC120.10978956999085088No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC120.10978956999085088No Hit
TACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGA120.10978956999085088No Hit
CGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGC120.10978956999085088No Hit
GCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA120.10978956999085088No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC120.10978956999085088No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA120.10978956999085088No Hit
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA120.10978956999085088No Hit
TAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGA120.10978956999085088No Hit
CTATAAGACTCTGACATTAGCGTATAGGCTGATACATTAGCTCGGATGCT120.10978956999085088No Hit
GCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTT120.10978956999085088No Hit
GTACATGGGAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGA110.10064043915827996No Hit
GTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTG110.10064043915827996No Hit
TTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGAC110.10064043915827996No Hit
ACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCT110.10064043915827996No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG110.10064043915827996No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT110.10064043915827996No Hit
GCATAGCAGATGCACGTTGAGTCCACGGGATAGAGTCTGAGACCTCGTGT110.10064043915827996No Hit
ATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTG110.10064043915827996No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC110.10064043915827996No Hit
CTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGGTTAA110.10064043915827996No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC110.10064043915827996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)