FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003677753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG43.0534351145038165No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT32.2900763358778624No Hit
GAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCC21.5267175572519083No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA21.5267175572519083No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG21.5267175572519083No Hit
ACATAAGCCTCCTTGATGTTGGTGGGTTCAGGTGGGACATTTGAAATGCC21.5267175572519083No Hit
GGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAA21.5267175572519083No Hit
GTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGTTA21.5267175572519083No Hit
CATACGCTCCGCTCGACCAACTACATCGACGAGCCAACCGGCCTGTACAA21.5267175572519083No Hit
GGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGG21.5267175572519083No Hit
CTTCATATTCAACACTGCTGATGAAATGGCTTTCATATGAAAATGCGTAA21.5267175572519083No Hit
TTGTTACTGCCACCGGGCAGGAGACACAGGGTCAGGGTTCCAGGGCCAAG21.5267175572519083No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT21.5267175572519083No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT21.5267175572519083No Hit
GTTCCATTCTGTAGGTTGTTTACTCTGTTGAGTTTCTTCTGCTGTGCAGA21.5267175572519083No Hit
TGGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGATGCTTGCT21.5267175572519083No Hit
GAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGG21.5267175572519083No Hit
AATTTATTGAGCGTTTTAGCATGAAGGTTGTTGAATTTTGTCAAAGGCCT21.5267175572519083No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC10.7633587786259541No Hit
ACCGAGATCTACACTTTTTTTTTCGTCGGCAGCGTCAGATGTGAAAAAAA10.7633587786259541No Hit
GAGGCGAGACAGGGACTAGGACTTGGCCGCACTGTGCCCTGAGTCGGGAT10.7633587786259541No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG10.7633587786259541No Hit
CATAAGGGCTATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCATTTCTTG10.7633587786259541No Hit
GTACATGGGCCGAGATTACACCTTGCCTCAGATTCTGTCGGTACCAGAGA10.7633587786259541No Hit
CTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGT10.7633587786259541No Hit
GTGTAAAGGTATCTGCTGCATCGAACTTTAAACTTCACGTTGTCCTTATT10.7633587786259541No Hit
GGCGAGAGCCACCACGCCCAGCCGCAGTACTTCTTTTTTAAAGACAAATT10.7633587786259541No Hit
TCATGAGGCCAAGAGATGGAGATCTGGCCAACATGGTGAAACCCTGTCTC10.7633587786259541No Hit
CTTTTATTTTGGGATGAAAGGGAACGAAAATTTGTTGAGCATCAAATGTG10.7633587786259541No Hit
CTTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG10.7633587786259541No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT10.7633587786259541No Hit
ATTTCTTGGTCTCTTCCTCCTTGGATAAAGTCTTGATGATCTCCTCCTTC10.7633587786259541No Hit
ACATTTATTTGTCTGAGGCTTGCAAACCTCTGGTAAGAAGCACAGAACGC10.7633587786259541No Hit
GTATATAGGCTGAGCAAGAGGTGGTAAGGTTGATCGGGGTTTATCGATTA10.7633587786259541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.7633587786259541No Hit
TAGCCCCGGCGCTGGATACGGTGCAGTCATAGCCGTCCTCCTCTCTGCTT10.7633587786259541No Hit
CTTCCTAGTTGGTACAGAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTG10.7633587786259541No Hit
ACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTA10.7633587786259541No Hit
TAGCCCCGGCGCTGGATACGGTGCAGTCATAGCCGTCCTCCTCTCTGCTG10.7633587786259541No Hit
GGTTATTGGGTATGGCCCGTCCGCTCTCATAGTCCGCGATCACCTGTGGC10.7633587786259541No Hit
GTGCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGA10.7633587786259541No Hit
TCATGAGGCCAAGAGATTGAGATCTGGCCAACATGGTGAAACCCTGTCTC10.7633587786259541No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGG10.7633587786259541No Hit
ACCCTCAGCCCTATCTTGCAGGTGATGGAGTAGAAGAAACGTCCAGGATG10.7633587786259541No Hit
GAGAAAGGGTACAGGATATTCCCAAACATAGAGGGAAACCTTCTAGTACA10.7633587786259541No Hit
GTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTTGTGATCCA10.7633587786259541No Hit
CTCTCTGTGCTAGTAACCACGTTCTCCTGATCAAATATCACTCTCCTACT10.7633587786259541No Hit
GGGGGATCTGGGTGGCGCATCTCCATGTTACCACATCCGGAATCAAAAAG10.7633587786259541No Hit
CTTCTTGCCCTCGAACATCAGACACCAGATTCTTGCCCGGTTTGTAGCCA10.7633587786259541No Hit
GCGTCAGAAGTGTATAAAAAAAAAAATAAAAAAAAAAAAAAAAGTACTCT10.7633587786259541No Hit
ACTTTGTAGCCTTCATCAGGGTTTGCAGAAGATGGCGGTATATAGGCTGA10.7633587786259541No Hit
TCCTGGGATTGGAGGTCATTTTGGGTTAGCCTTATTTCACTGCCATCTTC10.7633587786259541No Hit
CATAGAAACTGGGGGCGGAGCGATAGCGCAAGCGACTGGCATTGACCCAG10.7633587786259541No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG10.7633587786259541No Hit
GCCTGGCAGGTGCCCACCTCCCTGTACAGTCCTCCCTGGCTGCTCCCTTG10.7633587786259541No Hit
CCTTTACCTCTTCTAGATCTTTCTGTAGCTCTTCTGAAGCCATGGGGGCT10.7633587786259541No Hit
GTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGT10.7633587786259541No Hit
GACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTAGAACCAGGC10.7633587786259541No Hit
GTCATCATATTATCTAAAGTCAAGATGAAGAAAATAATGTAAAGAGCTGT10.7633587786259541No Hit
GCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAGGAGGGTGA10.7633587786259541No Hit
AAATTGACCTGCCCGTGAAGAGGCGGACATAACACAGCAAGACGAGAAGA10.7633587786259541No Hit
AAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAG10.7633587786259541No Hit
TCTCCCAGTAGGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGC10.7633587786259541No Hit
GTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTAACAGTTAAA10.7633587786259541No Hit
CAATAGAAGCCGGCGTAAAGAGTGTTTTAGTTCAGCCCCTCCCCAATAAA10.7633587786259541No Hit
GTAGATTAAGAGAATTAATTGGAAATTATTCATCCAAATGGCTGTCTCTT10.7633587786259541No Hit
CTATGATTGTAATTTTAGATTAGTTATGTGATTCAATGATTAGTTTCCAT10.7633587786259541No Hit
ATTACGTTTCGGCGCGCACCGGCGTGAACCCGGAATGGGTGTGGCGCTAT10.7633587786259541No Hit
GAATTTGGAGGGGCACCCATGTACTCTGCGTTGATACCTGTCTCTTATAC10.7633587786259541No Hit
AATAGAAAGAAAGGAAGGAAGGAAGGAAGGAGAAGAAAAGAATGAAAGAA10.7633587786259541No Hit
CGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGG10.7633587786259541No Hit
ATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCT10.7633587786259541No Hit
CTGCTGGGTTGGGACGCCTGCGGGCTGCTTCGGGCCAGGGTCGACCCGAG10.7633587786259541No Hit
ATTTTAGTCTACTAATTTTTTTGTTTTTGTTTTTGTTTTTTGTTTTGTTT10.7633587786259541No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10.7633587786259541Clontech Universal Primer Mix Long (95% over 22bp)
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC10.7633587786259541No Hit
CATCACAAAGAAGTCTCTGAGAATTCTTCTGTCTGGGTTTATAGGAAGAA10.7633587786259541No Hit
GCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCC10.7633587786259541No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG10.7633587786259541No Hit
TATATATCCTTCACTATTTTTATCTCTTTGCCCCAGCTTCATTATCTACA10.7633587786259541No Hit
GAGCCCAGAGGAGCACTGGAGACGAGGCCATCACTCCACTTCCCAGTGCG10.7633587786259541No Hit
GCTGAGTGACACTCAAGTAACTGGTGGTTTTTCCTGTGCAGCTGGCTGCT10.7633587786259541No Hit
GGCTGATGGTTTCGATAATAACTAGTATGGGGATAAGGGGTGTAGGTGTG10.7633587786259541No Hit
TTTAGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTA10.7633587786259541No Hit
AGTTTCATAAGGGCTATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCATT10.7633587786259541No Hit
CCTTAAAGACAAGGCCAAAGAGACTGCAGATGCCATCACTAAAGAAGCGA10.7633587786259541No Hit
TGCCACCACTGCTCTGATTTTCAGAAATTCTTCTGACTCTGATGGTAAAC10.7633587786259541No Hit
CTCAAGTAACAAGTTAATGAAACTACAAGGCTAAGCAGCCTTCTTGACTT10.7633587786259541No Hit
GGTCTGAGTGCCACTCCTTTGTCTACCACCCTATAGCAAAGCAGCATTTT10.7633587786259541No Hit
GTCAGTTTTCAACCTGGATGACCACCCTGAGCCAACATGTCAGACAGCCG10.7633587786259541No Hit
GCCATGGAGAAAGGGCTAATAGAAGCAGGTCCCTTGCCCAGACCCTCAGG10.7633587786259541No Hit
GCTCCACAGATTTCAGAGCATTGACCGTAGTATACCCCCGGTCGTGTAGC10.7633587786259541No Hit
CCTGCTGACGCTCGGTGCGGCCCAGCGAGCCGAAGTATTCGTTGAAGCCG10.7633587786259541No Hit
ACCTTACCACCTCTTGCTCAGCCTATATACCGCCATCTTCAGCAAACCCT10.7633587786259541No Hit
GGCTTGTACAGCAGCTTTTAGCACATCTATCTTTTTCTTCAGAAGAGGAT10.7633587786259541No Hit
TACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATACGGGAT10.7633587786259541No Hit
GATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCA10.7633587786259541No Hit
ATACGAATGGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAG10.7633587786259541No Hit
GGCAAGCATTTCATGACCAAGAACCCAAAAGCAAATGCAATAAAAACAAA10.7633587786259541No Hit
CCTCGACAGGATCGATAAACACGATGTCCTGCAGTCCGACTGGCGGCTTT10.7633587786259541No Hit
GCCTCAGAGTACTTCGAGTCTCCCTTCACCAATTCCGACGGCATCTACGG10.7633587786259541No Hit
CATGTAAATTGTGTCTTTTTGTTAAACTTGAAAGTTGATTGCTGCTAATA10.7633587786259541No Hit
GTTTGCTGCTGCTGTTTTATTTATTTTTACTGAAAGTGAGAGGGAACTTT10.7633587786259541No Hit
CCCAGGCTGGAGTGCAGTGGAGTGATCTCGGCTCACTGTAACCTCCGCCT10.7633587786259541No Hit
GTTCTTTTCTCGGCTAATTTGTGGTCAAGATCTACTACATTCGAAACATC10.7633587786259541No Hit
AGGCTGAGCAAAGCCCAGTGTCGTCTACACTGAGCACATTCCCCAGGACG10.7633587786259541No Hit
CCGTAACACCACTCTGTATTTTTCACTTTCAGTCTTCTCTTCAAACACTT10.7633587786259541No Hit
GATCTTTACTCAACTATGGTGAATGCATGGAACATAATACAAACATGGAT10.7633587786259541No Hit
TCTCTAACCAGTCACAGAATCCTGCTTCTGTATCAAGTGGAAGAAGAATG10.7633587786259541No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA10.7633587786259541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)