FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003677754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCA32.2900763358778624No Hit
GCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTC32.2900763358778624No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG32.2900763358778624No Hit
GACGATCAGATACCGCCGTAGTTCCGACCATAAACGATGCCGACCGGCGA21.5267175572519083No Hit
ACCTTTCTATATATACAAAAATTAACTCAAGGTGGATTAAAGACTTAAAT21.5267175572519083No Hit
GAAGAAAAAAGGGAAGAGAGGAAGAAGGGAGTGAAAAGCTACATCTGTCC21.5267175572519083No Hit
TTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCG21.5267175572519083No Hit
ATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCA21.5267175572519083No Hit
GGATCCTGGATTTGACATTGACAATTCCGATATTTCACTGGGTACCCTGA21.5267175572519083No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC21.5267175572519083No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC21.5267175572519083No Hit
CAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACT21.5267175572519083No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGTAGAGTGCTTAGTTGAACAGGGC21.5267175572519083No Hit
ATTCAATTCCCTCCTGACTTCATAGGCATTTCAAATGTCCCACCTGAACC21.5267175572519083No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG21.5267175572519083No Hit
GATTATAGGCACCCGCCACCACACCCAGCTAATTTTTGTATTTTTAGTAG21.5267175572519083No Hit
GTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAGCCCATGA21.5267175572519083No Hit
GTTTCTTCCTGTTGCTGCCGTGGCAGGAGCAGTCCGAAGCGCGTCGGGCT21.5267175572519083No Hit
GACCAATAACCGTGATGAACATCGCTGCAAATATCCTCAATAAAATACTG21.5267175572519083No Hit
GGTATCAACGCAGAGTACATGGGCCTGGGGAGACTGAAGAGCCACCAGTT21.5267175572519083No Hit
GCAGCGAACTAAATTAATTTTTTTTTTTTTTTTTTTTAAAAACAACAAAA10.7633587786259541No Hit
GCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTA10.7633587786259541No Hit
GTGAGTGTCTCTAGGGTGATACGTGGGTGAGAAAGGTCCTGGTCCGCGCC10.7633587786259541No Hit
GTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT10.7633587786259541No Hit
CATAAGGGCTATCGTAGTTTTCTGGGGTAGAAAATGTAGCCCATTTCTTG10.7633587786259541No Hit
GTTATAATCTGTGATGCTGGTCTGAAGTCTGAATATTCCAAGTTGCTGAC10.7633587786259541No Hit
TGTAATCAATTGCACAATGAAATAAATAGTCACCATGAAGTGTCTAAGAA10.7633587786259541No Hit
NTTTTCTGGGGTAGAAAATGTAGCCCATTTCTTGCCACCTCATGGGCTAC10.7633587786259541No Hit
GTGGGGCATGGGAATCAGCCACAACCAGCACTGCATCCCGACTCAGGGCA10.7633587786259541No Hit
CCCTGATGAAGGCTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAG10.7633587786259541No Hit
GTATCAACGCAGAGTACATGGGAAGCGCAAGTACCCACGTAAAGACGTTA10.7633587786259541No Hit
CCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGCCGCGGA10.7633587786259541No Hit
CCGACCGCCGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTT10.7633587786259541No Hit
CCCAAAGGGCTGGGATTATAAGCATGCGTCACCATGCCCAGCCGAAAAAC10.7633587786259541No Hit
GGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTC10.7633587786259541No Hit
CTCCTGCTCTCTGATCTTATTGCTTATAAACTTTGGTGACGGTAGTGTGT10.7633587786259541No Hit
GTACATGGGGGGCCAGGCGCCGTGGCTCACGCCTGTAATCCCAGCACTTT10.7633587786259541No Hit
GGTTATTTTTATTTGTGGAATCAGGATTTAAAATGCTGCTTTGCTATAGG10.7633587786259541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA10.7633587786259541No Hit
GTCCTGGATCAAGTAGCTCTTGTTTGAACATTATCTCTCTAAAAATGGGA10.7633587786259541No Hit
CTCCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCC10.7633587786259541No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA10.7633587786259541No Hit
GCCTTAAGCACAATTAATTAAAAGTGAAACGTAATTGTACAAGCAGTTAA10.7633587786259541No Hit
GTGTGGAAGGGAAGACGCCAGCCTTTTGGCTTCAACTCAAAGGCGCAAAA10.7633587786259541No Hit
CTACAAAGTAAGCGCAAGTACCCACGTAAAGACGTTAGGTCAAGGTGTAG10.7633587786259541No Hit
GTCCTGAAGACGGGGCTAGATATTGGGAGAAACAAAGTGGAAGATGCTTT10.7633587786259541No Hit
AAGAAAAGGTGAATCTCCTTGAGGATCTTCTGTTTAAGGAAGCAGGGAGA10.7633587786259541No Hit
CTTCAGCGCCTTGATCGCGGGCAAGGGGGCCAGGTGCGGCCCGAAGATCG10.7633587786259541No Hit
GTCCAAGCCGGTGCTGGTGCCCGACGTGTCGCAACGCGACATCGAGATCC10.7633587786259541No Hit
GCCGTGGGGTTCCTTCGGCATTAGTTCACGGTTTTTGAGAGAGGCCTTAG10.7633587786259541No Hit
GCGCTACACCACGCACGAAAGCAACCGCGAGACCGACGAGAGCGAGTGGA10.7633587786259541No Hit
ACTTTGTAGCCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGA10.7633587786259541No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG10.7633587786259541No Hit
GTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATA10.7633587786259541No Hit
AAAGGGTACTGAAGATGCACTGTTAAAAAACCAAAGACGGGCGCAAAGAT10.7633587786259541No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT10.7633587786259541No Hit
CATTTAACTAAAACCCCTACGCATTTATATAGAGGAGACAAGTCGTAACA10.7633587786259541No Hit
ACCCTGTCTCTACTAAAAATACAAAAATTAGACAGGAGCCTGTAATCCTA10.7633587786259541No Hit
ATTTTCTGTGTGCTCATCAAGTTCAGAAAGGATCTCTCTGTATTTTGGCT10.7633587786259541No Hit
GTATCAACGCAGAGTACATGGGTGCCCCTCCAAATTCCTGTCTCTTATAC10.7633587786259541No Hit
TGCCAAAGGAGATTAACATTTCAGTCAGTGGACTGGGAGACGAAGACCCA10.7633587786259541No Hit
AGCCGTGTATTTGTCCTTTTAAGGAATGTGAATTCTGCCTTTAAATGCTT10.7633587786259541No Hit
TCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGCTCAG10.7633587786259541No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG10.7633587786259541No Hit
GTTTCCATCCAGTCTGGTTTAGGTGCTCTTCTTTTCTTCACCCAGTAAAT10.7633587786259541No Hit
GTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTC10.7633587786259541No Hit
GACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCA10.7633587786259541No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC10.7633587786259541No Hit
CTCCAGCCTCAGCTCTCAGTTGCAGCCCTGCTGCTGCCTGCCTGGACTTG10.7633587786259541No Hit
ATATGGTATTAATAATAATGGGAATAAAAGCTAACATTTATTGAGAATTT10.7633587786259541No Hit
GCACATGAACCACCCATGAATTACAATTACAGCCAGGGAAGGGAAATCAG10.7633587786259541No Hit
CTTTAGTTTAATTCGGTCTCAGCTATTTATCTTTGTTTTTATTGCATTTG10.7633587786259541No Hit
CAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGTTT10.7633587786259541No Hit
GTTCTGTAATCGATAAACCCCGATCAACCTTACCACCTCTTGCTCAGCCT10.7633587786259541No Hit
GTTACACTCTGTGCAAAGCATTCTATGTTTTCCCTCTAAAGTAATCCTCT10.7633587786259541No Hit
GTATCAACGCAGAGTACATGGGGGTGGCGGCGGCTGCCTCGCGAAGGTTC10.7633587786259541No Hit
CACTAATCTAGGCTTAACTTTTGTTCTTAATCTGGGATATTTTTAGAAAT10.7633587786259541No Hit
GAGCAGGGCCTGCTGCTTCCCTGGCGCTGCTCCCAAAGATTTCTGACTCA10.7633587786259541No Hit
GACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAAATGTTGAGCCGTAGA10.7633587786259541No Hit
CCATTTGGATGAATAATTTCCAATTAATTCTCTTAATCTACCTGTCTCTT10.7633587786259541No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC10.7633587786259541No Hit
CCAATTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC10.7633587786259541No Hit
CTATGTTTTGGTATATGTATATTTATGACAATGTGGTTAATTTTTTTAAA10.7633587786259541No Hit
GGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAA10.7633587786259541No Hit
CACCTGAACAGTCCCGAGCAGCCAGCTGCACAGGAAAAACCACCAGTTAC10.7633587786259541No Hit
GTAGTCATGGAGAAGGGTTCATCAAACTATGGCCTGCAGGCCAAACTTGG10.7633587786259541No Hit
CTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTCATCCAAA10.7633587786259541No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT10.7633587786259541No Hit
CTCCTCAACCATGTAGGGATTGGGTATCCCTGATTTGCTCTTGGTATTAG10.7633587786259541No Hit
CAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTG10.7633587786259541No Hit
GTTGAGGTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTC10.7633587786259541No Hit
TCGCCATCCTGCTCCTCGGCGTGGCGATCTACATTGCCGCAGACGCGCAA10.7633587786259541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAA10.7633587786259541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAACCCAG10.7633587786259541No Hit
NTGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATA10.7633587786259541No Hit
GTTCCATGATGGCTGAGCTGTGATGACTGATGTTGCCAGGAGCTTTGTCT10.7633587786259541No Hit
ATCCCTCCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTAC10.7633587786259541No Hit
GCCTGGGGCAGAGGAGGGCTGCACCAGGCGTCCTGGGGAATGTGCTCAGT10.7633587786259541No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA10.7633587786259541No Hit
GGAAAAAAGCCCTATCTTACCTATAGAGGAACAAGAAAAATAATTACATC10.7633587786259541No Hit
CCTTTTAGCAGTTCTTGTGTTGTTGGCTTGGTAGTGGCAAATTCTCTCAG10.7633587786259541No Hit
GTGTGCTTTCAACTCACCAAGATAAAGATTTCTCTTGATAGAGCAATTTG10.7633587786259541No Hit
TCTACTACCAACACGCTTGATTTCTGTTTGGCTTTTTACTGTGTATTTTG10.7633587786259541No Hit
ACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTG10.7633587786259541No Hit
GCACAGAGACGAGGAGAAGATGTGGAGACTTCCAAGAAATGGGCTGCTGG10.7633587786259541No Hit
GCTCAGAAGATACCGTGAATCTAAGAAGATCGATCGCCACATGTATCACA10.7633587786259541No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTAAACCAAT10.7633587786259541No Hit
CCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTA10.7633587786259541No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)