FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003677807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157
Sequences flagged as poor quality0
Sequence length140
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGCGCGGTGGGACGTGTACGGGCAATATGGCCGCGCCGACCTGCACG42.547770700636943No Hit
GCACGGATTGGATCAGTTGATGCAGATAGGCCGGACCGCCCACCTTCGAG31.910828025477707No Hit
TCACGCGGGTGAAGACGATGTAGGCGGCGACGGTGGCGAGCGTGGCGACG31.910828025477707No Hit
GTGCTGGACGTGGTGGTCGACACGGGTATCCTCTCCAAGGACTGACCAGT31.910828025477707No Hit
GGTCTGGTGCGCACACACGGAGGAGTCGTCGAGCAAACCCGCGTACCCGA31.910828025477707No Hit
TCACTACGGTGCGCTGCAGGGTCTCAACAAGGCTGAGACCAAGGAGAAGT31.910828025477707No Hit
GTCTGGGCGTGGTGGCGGGAGGGATCGTCGCCCTGGTCCTGGGACTGGGG31.910828025477707No Hit
CCGCGACGTCGCGGCCGAGGCGGTGGTCGCGGGCCTTCCAGACATCGGCC21.2738853503184715No Hit
ATCCCGAACAGCTGATCCCACACAGCCTTGCGGTATCGCTGGCGTACCAG21.2738853503184715No Hit
CCCTTCACACCATGTACCCACATGCTTCAGGCCTAGTCAGACCGAACTTT21.2738853503184715No Hit
ACGAAGGCCCGGCCAACGGCACCGATGAAGGCCAGGCGATGGCGCAGATC21.2738853503184715No Hit
AGCTTGTGTTGCTCGTCATATCCGAATGAGGTTCCCGGCCCCTGCCATTT21.2738853503184715No Hit
GGCGCACCTCCAACGGAAAATTCACGGACATAGAACAATTGGGTGAAGTC21.2738853503184715No Hit
GCCCTGATTTTCCGCTAGCAGTGGGCCCGACGAGGCAGATGACCGGCAAA21.2738853503184715No Hit
GAGTATATGAGTCATCGTGGCGTATTTGTGGGCAACGGCGGTTGGTCAGA21.2738853503184715No Hit
TGGCAGTGCTGATCGACGTATTCACTGGCCACCCAGCCCACTCCACAGCC21.2738853503184715No Hit
GTGGAGCAAGGCAGTGGCCAGGTTTGGCATGATCTTCACGGTGTTCATAA21.2738853503184715No Hit
GTTCGGGCTCCTCGCGTTCTTGCCGCCAGGCGATGCCCACCGGGTACTCC21.2738853503184715No Hit
GACGAGGTCATCGCGCTCATCCGTCGCTCCCCGAACACTGAGGCCGCCAG21.2738853503184715No Hit
ATGCTGCGGATCGTGCCACATACCCTGGGCCTTAGCATAGGCCTCGACGA21.2738853503184715No Hit
TCCCAGAACGTCGCCTGCTATTGCAGTCTCTTGACGGGATCTGCCCGGTC21.2738853503184715No Hit
ATCTCCAACAGGTCGGCGAGAAGCAGTTCTAGCCGCTCCATCTGGGCGCG21.2738853503184715No Hit
AAGCTGTACGGTGCACCGTTGACACGGATGATGTTGTTCACGATGCCCAC21.2738853503184715No Hit
ACCAGGTAGGGCTTGTCGGTTCCGTTGGGGGTGGCCACCAGACGCGCGTA10.6369426751592357No Hit
GCCGTGGCGGCCTGCGCGGTGCTGCTCTTCTTCCTGATGCGCACGACTGA10.6369426751592357No Hit
TCAGTGCTACGTCCTCGACCCGATGCTGGCTACCGGCGGTTCCTTAGGCG10.6369426751592357No Hit
GGGTGAGACAGGGCGAGAGTGAGGTCGATAAGGGACATCCGGGATTCAGG10.6369426751592357No Hit
TCGTCAACCACGGTGACGGTCTGTTGACGTGGAGCGAGAAGAAACTGAAC10.6369426751592357No Hit
GCAGCGGTGGTGCAGCGGCGCAATCAACCAGCAGCGATCAAAGCGGTGCG10.6369426751592357No Hit
TCAGAGATGCTGAAGTTCATAGAAGTTTTATAGATCTTGCAGATGTTAAG10.6369426751592357No Hit
TGCCCAGATCGAGGAGGCGAAGAAGGTCTGTGCCCGGTGCGAGGTTCGCG10.6369426751592357No Hit
ATCATAAACGGCGTGACGATACATCCTAACCGCGTCCCGAATCCTCCTCG10.6369426751592357No Hit
ACCTCAAGGCTTCCCCCGGCCCGGCCTATCACTACAACAGTGGCTGGGTT10.6369426751592357No Hit
TGGCGGTATTGGTGCGCACATCCACGCCGCGCTTCTGCAGTTGGCGGCGG10.6369426751592357No Hit
GTGATGGCCTGACTCCGCGCGCCGTCAAGCAGCTCATCCGGCTGGGTATC10.6369426751592357No Hit
GTCCCGGCGGCCGGCAAGCCCACTTTCGACCTCGACGAGAACAAAATCGA10.6369426751592357No Hit
GTCAGCAGCAGTGCCGCTTGGGTTTCGTGCAGATCCCCGGTTGGCAGGCC10.6369426751592357No Hit
CTCGCGCCCGTCCACGGCGGGCGGCACTTCGCCACCTTCGTCGGCCGGCC10.6369426751592357No Hit
GTACAAAAAACGGGAGGCGCAACCCGAGGGAAGGGACTCCTGTGACGATC10.6369426751592357No Hit
GCGCTCGAGGGAGCGACGGGCCTCCTCGTCAAACTGAAGTTGCTTAGCTG10.6369426751592357No Hit
AGGGGACATCCCTGCGTTGGCAGCCTCCCAATTCTTGGCGTCGTCAACAT10.6369426751592357No Hit
CGCATGGAATGCTCAAGGTCCTTCTTGGCCTTCTTGGCAGCCGCCTTTGT10.6369426751592357No Hit
AGGCGCCACCGTCCGGCCACTGGCGCGAAGAGCAGCCATGAGACCGGTAG10.6369426751592357No Hit
GTCCTGGGCAAGGTGTTCGACATGGGCAACGGACGACTGCTCAGGCGACA10.6369426751592357No Hit
CTGCTGCACGGCGTCACCGCTGCGGACGAGGCTGGCGCTGTCTCTTATAC10.6369426751592357No Hit
GACGAACAGCATTTCCATTTTCGTTCCGTCCAGGCCGGCGAAGCCGATCA10.6369426751592357No Hit
TTCCCCGCTGACTCCTCAGCGCGAACTACTACCCGGAGATGTCCTCAACT10.6369426751592357No Hit
TGTCTCGGTTGTAGTACATCCGGATGGCGGCCGAGTTGATGTTGGTAGTC10.6369426751592357No Hit
CTTTAGGATGGCTCCCGACTGGGCGACTATTCCGCTTGTCACCATCGCCA10.6369426751592357No Hit
CAGTTTCAGGGCGAATATCTGGGCAGTGTGCTCAGTGGCTTCTTCGAGTC10.6369426751592357No Hit
GGCTGTAACCATAAAGAGTTCGGCGCATCCCGTGGGATGATTCGGGCATA10.6369426751592357No Hit
CTGCAAGCCCATCAACCAGCTCAGCGAGACCTCGATTGGGCGCCGTCAGC10.6369426751592357No Hit
AGGTAGAACAAGGCGCCGAGGGATTCGCGGTAGTGCTGGCCTAGCAATGT10.6369426751592357No Hit
ATGCACAACGCACACCCACTGCCCAGCCTCGTGAGCCTCTGAGACAGCCT10.6369426751592357No Hit
GAACTATGCTTTCTCGTTGTAAGTTTCCTGCCATGTTCCTAAACAGGGGA10.6369426751592357No Hit
ATAAGCTCCCGGGCAAGAGCCTCGGCAGGCGTTTCACCGGCTTCAATGCC10.6369426751592357No Hit
CCCACGGTTCCGGCCTGGCCTGGCTCCCAACCCTGGTGAGCGTTGCGCTC10.6369426751592357No Hit
GGTAAACGATAGCGGGCGGCAGCAAATCCGAGTTGTCGATCTGATTGAGC10.6369426751592357No Hit
GCATACACCTGAGCCGTCACACCGGCGCCAAGGGAAAATCCGGAAATAAG10.6369426751592357No Hit
ATCCAGACAACACCAATCAACGACATTTTTACAATTTGATCCCCAGATCC10.6369426751592357No Hit
ACGTATATGCAGACGGTTGCCGTGACTGGAAGATTGTCGAGCAAGGACCC10.6369426751592357No Hit
AGCTTACGCTACGGCCATCACTGGCCACGGCCTTAGCCAGACGACCCTGC10.6369426751592357No Hit
GTATTAGTGTGAATATTTATTGCTGCCTCGCGTCAGTGGGGGTCTGGTTC10.6369426751592357No Hit
TTCGACATCGTGCGCGAGGCCGGCGATCTGCTGGGGGCATCTCTGGGAGC10.6369426751592357No Hit
CTCCAGCCGCGTGGCGAAAAAACAATGTTTCCATCAATGACTTCGCCTGT10.6369426751592357No Hit
GTCCGGTACGTAGCGAAGGGGGAGCATCCCAATCCGGCCGACACGTGCAG10.6369426751592357No Hit
GCTCAGGGTAAAGCGGAGTTGCAGCCGCTCGCGCTCCCTGTCGGCCGCCC10.6369426751592357No Hit
GACCAGGAGATGTCCCCATCACGCCAGCGATCATGAGGATCCCAAGCACC10.6369426751592357No Hit
GGTGACGAATGAGCAGCGAGACCGGGTTCTCGACCACATCAACGACGCTT10.6369426751592357No Hit
GTCGTATACAGCCTTCGGCCATATCCGCCGCTGGCAATGGGACTTAGACG10.6369426751592357No Hit
GACTTTAGGTCGCGGTGGTCCGGTGGTCTGGTTGAGTGAAGCCGATGCCA10.6369426751592357No Hit
GCTGGGACGTTCGTCGCGCCCTGGACCGCGCTATGGATCGCCTTGACCTA10.6369426751592357No Hit
GCTGCGGCGAGGGTGCGCGCCGATGGGTTGGCGAAGAACACCTGGGAGAG10.6369426751592357No Hit
GTTCGGGAACGGGCTCGACATCAGTCTCTGGACCGGTCTTGAGGGTCGGC10.6369426751592357No Hit
GTGCTATTCCTCGTGCTTTTCGTCATCCAGAATCTGCGGCTGCACGCCGC10.6369426751592357No Hit
TTCTTGTACAGTGTGATCTTTATATCAATTTCGGCAGCTCAGCGCGCACC10.6369426751592357No Hit
GTGCTCGGCCTCAACAACCACGATCACGCCACGAGCGTCCAAATGTTCTA10.6369426751592357No Hit
CAAGGACACGCCGGCCGACCGCGTCCGGGTGCATGAAGACGGAGCTAACC10.6369426751592357No Hit
GGTTACACCCGCGACATCGCCCTCGTAGGCCCAGCCAAGCTGGTCGAGGG10.6369426751592357No Hit
CGTGGTACCTCTCGACTTTGATGATCTCAGACCGGTGACGGGCTTCCTCG10.6369426751592357No Hit
GTGCTCGGCCTCAACAACCACGATCACGCCACGAGCGTCCAAATGTACTA10.6369426751592357No Hit
CCCTTCACACCCTGTACCCACATGCTTCAGGCCTAGTCAGACCGAACTTT10.6369426751592357No Hit
GTCGTCAGCAGGGTCCCCGCTTGGCTCTCGACCATCAATGATGTCAATGC10.6369426751592357No Hit
GGCCAGTGGGCGATGACAGCGGGAAGGCCGTCGTTCTCCGTGACGACGGT10.6369426751592357No Hit
CACCAAAATCGTGCAACTCGGATACGGCAACGACGGCGAGGTCGAGGACA10.6369426751592357No Hit
ATGCTGCGGATCGTGCCACAAACCCTGGGCCTTAGCATAGGCCTCGACGA10.6369426751592357No Hit
GTAGTAAATGTCGAGGTCTTTGGTCTCGATGCGCTTTGCCATATGCAGCT10.6369426751592357No Hit
TTTTGGGGCTGCTTGACCCGTGGCTGGGTGCCAGCCGCCTCCCCCACCGC10.6369426751592357No Hit
CATCTCGACCTCGCCCCGAGCGTCCTGTGGACCAAAATTCTGCGAGTCGA10.6369426751592357No Hit
TCACCGCGTTGACCGCGAGACAGCGCTTCAAGCCGGGCACCAACCTCGTC10.6369426751592357No Hit
CCTTGGACGCACCGATTGTGCAGACCTGTTTGGTCATGGTGCGATGGGCG10.6369426751592357No Hit
TGGCAATTGCGACCAGGGCGAAGAGGGCTAGTGTCCAGCGCCCCGATTCC10.6369426751592357No Hit
GTGGAAAAGCAGCTTCCTCATGCTGTTTTCACCGGCGGTCTCGGCGGGGA10.6369426751592357No Hit
CTCGCACGGAGAAACTCTTTAATCTTTGACCAACAGTTTTCAATTGGTGA10.6369426751592357No Hit
GGCCAGTTCAGCCATTCCGGCAGTTCGCAAAGCGTCACGTACTGCGATGT10.6369426751592357No Hit
CGGCCCCCCTGCCGTATGCTGAGGCCAGTTTCTAGTCGTGGGGGTTTGCA10.6369426751592357No Hit
GTCGAGGACGTCCGGCATCCGATCATCGGTGTGAAACGCGACCGCGGCAC10.6369426751592357No Hit
GGACCTGACCTGGCCGCCAACGCCCTGCCCCGCTTCGAGGACGACGGGAC10.6369426751592357No Hit
GGCCCACTCTCGGCCACCGCGTGCGTCCTCTCAGTAAGGACGCCGAGCTG10.6369426751592357No Hit
CTACGACACAGATGGGTGCCAGTGCCACCCATGAGGAGGGGTCACGTGCA10.6369426751592357No Hit
CTGATGTGGAGCAGATGGTCGCTGATTACGCCGAGTCCAGGATCGACCAC10.6369426751592357No Hit
TGACAACACTGACCGGAGGCGCGAGGTATGACGACATGCAGGCTATTGTG10.6369426751592357No Hit
ACCTCACACTGCCGAGCGCGCCCGGCAGTGGTGGCGTCGGATGCGCGGTC10.6369426751592357No Hit
GAACTATGCTTTCTGGTTGTAAGTTTCCTGCCATGTTCCTAAACAGGGGA10.6369426751592357No Hit
CCCCACTGCTTGACGCCATGCGCACCGAGGTCACCTGCGGCCAGGTCCTC10.6369426751592357No Hit
ACAATGCAATGTCCATCGATGTCTACACCCAAGTTATCGACAACAAGCTT10.6369426751592357No Hit
GTCCGCGAGAATCCAGCGTCCAATAACAACCTTCTGCTGATTACCACCAG10.6369426751592357No Hit
TCGAGCCCCTCATGCATCACCTTGACTGCGACGATGCGGGGAAGACATAG10.6369426751592357No Hit
TCTCTGGGATGCATTCAAAGCAGTGTTTAGAGGGAAATTTATAGCACTAA10.6369426751592357No Hit
GTCGCCACGGTTGCCGAAGACCATCTGCATGACATTGACGGCGGTACCCC10.6369426751592357No Hit
ACTATAAAGATCGTGAACTCGGGACTCTCTGTGTCGCCAGGGCCCGCACC10.6369426751592357No Hit
CACGTGATGTGTGCTGACGCTATGGCGGTCTTCGCTGACTTCCGGGCTCA10.6369426751592357No Hit
GTCTCATTCTTCCCCTATGTGATTCCGGCAATCGCCATCGGCATCATCTG10.6369426751592357No Hit
GTCAATCCGTTGACGGTGGTGGTGCGCGGCCATGTACCGGTGATGGCGGG10.6369426751592357No Hit
CTGCGTAGCGACGACCCCGAGCTCATGCAGAAAATCGAGGACATCACTCA10.6369426751592357No Hit
ATGGTCACCCCCGACATGATCGGGCACACCATCGGCGTGCATGACGGCCG10.6369426751592357No Hit
ATCCACAACTCTCCCCCGGCTGCCTCAACCCGTCGACGCATACCCGTTAA10.6369426751592357No Hit
GTCATCGCCACCCATATCCAGCCCCGCACCAGGGCCAGAACGCACCTGAA10.6369426751592357No Hit
TTCCTTCGGTGCCCCGGTCATCGATCCACCAGGGAAGGTCGCTCGCAGTA10.6369426751592357No Hit
CCCCCAGACCGGCCGACAGGTCGGTTGTAGGAGTTCTCGGAAGGACCGCA10.6369426751592357No Hit
GGTCACCATCGAGCTTCTTGACAAGGGCTTCAATAACCTCATAGTCGGGC10.6369426751592357No Hit
ACCCCGTTAACCATTCGTCCGGGAAAGAACCAGCCGTTTTGTTTGTTGGC10.6369426751592357No Hit
ATCATAAACGGCGTGACGATACATCCTAACCGCGTCCCGAATCCTACTCG10.6369426751592357No Hit
ACCTTGGGCTTGGTTTTACAAGCTTCGTCACCACACTGTTCGTCGGCGTA10.6369426751592357No Hit
GTCCCTCGTCATGCCATACTTCGAAGCATGACTACGCTTCGACGTACACC10.6369426751592357No Hit
CTCCAGGACAGGGCGTCAAACGGGCAGGACTCGATGCACATGCCGCAATA10.6369426751592357No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)