FastQCFastQC Report
Tue 10 May 2022
EGAF00003677809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173933
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG6850.3938298080295286No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT5800.33346173526587825No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC5310.30528996797617475No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG5300.3047150339498542No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG5270.3029902318708928No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG4860.27941793679175314No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC4730.2719437944495869No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT4300.24722163131780628No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA4150.23859762092299908No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA4130.23744775287035813No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG3650.20985091960697508No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGC3530.20295171129112935No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG3260.1874284925804764No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGCCAGAGCATC3100.17822954815934872No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGCCAGAGCA3090.17765461413302822No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC3080.17707968010670774No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT2920.16788073568558007No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT2770.1592567252907729No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA2760.15868179126445242No Hit
GAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG2660.1529324510012476No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT2650.1523575169749271No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT2640.15178258294860664No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC2480.14258363852747896No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA2440.14028390242219704No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC2380.13683429826427418No Hit
CATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGCCAG2280.13108495800106937No Hit
GACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGC2240.12878522189578745No Hit
CCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTG2220.1276353538431465No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA2210.12706041981682603No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT2090.12016121150098026No Hit
GGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATC2070.1190113434483393No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC2060.11843640942201883No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC1950.11211213513249355No Hit
TTTGTGATGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGAC1880.1080875969482502No Hit
GCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCA1820.10463799279032732No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT1800.10348812473768634No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT1800.10348812473768634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)