FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003677827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGATGAAGCCGAACGTGTACGTGAAAAAGCGCGATTAGACGCAGA33.6585365853658534No Hit
CGTCCACCAAGACGGCGATAACAACTGGCGGGGCGGACACCTTGTCCAGT33.6585365853658534No Hit
GTGATAACACACTAATTCATTAATCCATTAATTCATTAATCCATTAACCC22.4390243902439024No Hit
GGATGACGGCCCGGCACCCAGCACACAAACAGCGACAGTCCCGAGGGTTC22.4390243902439024No Hit
TCGTCACGCTGCATGACGCGTCGACCATCGCCGCCGTCATCGTCGAGCCA22.4390243902439024No Hit
ATTCCATTGTACATATACACCACATTTTGTTTATTCATTCATCTGTGGAT22.4390243902439024No Hit
TTTGTATTCTTAGTAGATATGGGGTTTCAGCATGTTGGCCAGGCTGGTCT22.4390243902439024No Hit
ATATCAAACTGCAGTAAAGCTCCATGGGGTCTTTCCGTCCTGTCGCGGGT22.4390243902439024No Hit
GGGCCCAGCCGACCGACCTGTCACAGGCCCCGATGCACTTCGAATCCGAG22.4390243902439024No Hit
CCCGCCCGAGACCGAGACAGAGCGCTTCGCCGCGCCGATATGGCTGCACA22.4390243902439024No Hit
GCTTTATATTCAGATCACTTTAGCTTTTATTTTATTTCTGACTTGTCTTT22.4390243902439024No Hit
GATATTCGGGTGCTGGACCCGGCAGACGAGCACGGCGTGGCCGGTAAAAT11.2195121951219512No Hit
CTTTGGATCATTGCATCAATTAAGTCTAATTTGATGAGTTGGTCGGAAAG11.2195121951219512No Hit
ATCTAAGACAGACCCAAGCTGTGTCCTGTGAGCAGGCTCGGCGCCACTGC11.2195121951219512No Hit
GTCCTGGGTCGTAATTTGTAGGGTTGCGATTTGGTGCAGTAGCTGCATCC11.2195121951219512No Hit
TTAACGATTCGCACGGCGGTTTCCGCAACCTCATCCCCGATTCCATCGAC11.2195121951219512No Hit
GGTGTGCGCTGAGTAATTCCTTTTTGTGTGGGGAAAACACACACACACTT11.2195121951219512No Hit
ATCCTGCCGGCAGGCCCGGTGATCGTGGCGGAGCAATTGCCCGACAGCCA11.2195121951219512No Hit
GTTTCCAACGGGGTGATCAGGAAGATCACCGCGATCGGCACGAAGGCGAT11.2195121951219512No Hit
GTTTTAAACATATCAGACTACCAGTATGGAATACTCGACCCGTAATTAAC11.2195121951219512No Hit
GTCCTTCCACGGCACCACCATCGGCCTGGCCCCCCTCATGGCCATGTGCT11.2195121951219512No Hit
ACATTGATACCCGGGGCATGAGCAAATCGGAAATCAAGAAACGCCTGATG11.2195121951219512No Hit
AAATGAAACTGGAGAAATTAATGGGGTTATGAACATAAGCTTTGTCCTAG11.2195121951219512No Hit
AAACAACCCCATCAAAAAGTAGGTGAAGTACATTAACAGACACTTTTAAA11.2195121951219512No Hit
CGACCTGGCCTGGCTGCGCGCGCAGAAGCTGGATGCCGCCATCAGCGCCC11.2195121951219512No Hit
TGCTGGATCCATTTCCTCAGATTCCCCGGTAAGACGGTTTCGCAGTTCCA11.2195121951219512No Hit
GTCTAGGTAGGCCTAATCTAAGAAATGAGCCCTTTAACAGCAGAAAGTAT11.2195121951219512No Hit
ATATGGTTTGGCTCTGAGTTGTAATTCCCAATGTTGCAGGAGAGGCCTGG11.2195121951219512No Hit
CTATACCGCTAAAGATGATTCAACTAGCTGATCGGTAAAACGCATTCTGC11.2195121951219512No Hit
ACTTTCTAGGGCTCTAGTGATTTTGTGTGTGTGAGATTCATTTTGTGTCA11.2195121951219512No Hit
GCTTGAGACTGATAAACGCCAGGCCGTTGTTTTGCCCCTGACCGCTGAAG11.2195121951219512No Hit
GGATGTGGGCTACAACAGGAGAGGCAGCTCACTGCTGCTGAGGCAAAATC11.2195121951219512No Hit
GACCGGCCCATCGCGTTCCGGATCACAGCGACCACGGTCATGAACGCGTT11.2195121951219512No Hit
GCAATACGCTAGCTGGAAAGGCGTGCGTTACCGCCTTGGCGGCGAAACGA11.2195121951219512No Hit
CATATATAGTAATAAAGTGGAGAAAAGTCTCAGTTGCCTAATGAGAAAAT11.2195121951219512No Hit
CAGGGACACAGTCCCAGCTCTCAAGTGTGGGTCTTTCCTAAGCAAATTGA11.2195121951219512No Hit
GGCCGACTGGACATGATGTACCTGCCGCCGGAGTCAGTGACCGGCTTCCT11.2195121951219512No Hit
GCCACGAGCTGATGCTGGAGTTCAAGCGGCGCCGGGAGGAGCAGCACATC11.2195121951219512No Hit
GCACGGCGGCAATCGCCGCGGCCAGGGCCTGCTCGGCGGCTTCCTTCAGC11.2195121951219512No Hit
GAGTACTGCAGGAACTTCGGGCGCGAAGGCTCAGCCAGGCGCACGGCAGA11.2195121951219512No Hit
GGTGAGCAGCAGGAAGCCCTGCGCGAGATCCTGCACCAGCACCAGCGGCG11.2195121951219512No Hit
ATCCTCTTTTATTTCCTTGAGCAGTGGTTTGTAGTTCTCCTTAAAGAGGT11.2195121951219512No Hit
GCGCAGGTGCGCGGTGGGTTCTCCAACGACACGCACCGTCCGCTGGGCCC11.2195121951219512No Hit
TGTGTCAGAGGCTTTTTCATTTTCCCAGAGCCAGTGCTGGCTGGCGTGGC11.2195121951219512No Hit
CGATGGCTACCGTGATCGCGTCACCGCCGAGCTTCGGCACGCCAGGATCG11.2195121951219512No Hit
TGTCTGAAATCGAAGCCGTGTGCCAGCACCTGGCGGCGCTCGACCCCCTG11.2195121951219512No Hit
CCTAATAATCCCATAATCCCACCTGCACGGTGTACATCTTCCATGTGGTA11.2195121951219512No Hit
GAACTGGCGACACCGCTGGGTGAACAGATCCAGAAGCTCCTGGGTCTTCC11.2195121951219512No Hit
GTACTTGGCTGTGGGCGATGCCAGCTTGTAGCTGTTGTAGGGAATGTAAA11.2195121951219512No Hit
AAATTCCTGTTGTGGAACAAGGCTGGACGGTGATGGGAATGCTCAGGATA11.2195121951219512No Hit
TAGTGGGGGGGCGCCCATCGCCGCGCGGACTTGGCCGCGCATGCCGAACT11.2195121951219512No Hit
GTGATGTTTTTATGTGAAGATAATGCTTTTCAACCATAGGCCTCAAAGCG11.2195121951219512No Hit
CAGTAGTACTTAAACCAAGTGATACTACGCCTGAAAGTACGCTTTTACTG11.2195121951219512No Hit
CCTGCGGCAGGCGCGTCGCCTGCTGGCCCAGGCAGGTTGGACATACTCCG11.2195121951219512No Hit
GCTCATAGCACAGAAAGCCAATCACTGAGACAATGGATATAGACAGGGAA11.2195121951219512No Hit
GTTACGTTTTGCGCGACGCAGACAACTTCAAGGCGATCATTATCGCCACG11.2195121951219512No Hit
GGTTAGACCTCAATTTCTGCAACACCTCCTCTAGGAAAGGCTTTCTTGAC11.2195121951219512No Hit
GTATTTACGCACATAGCTCACCAGGGACACCAAAAGTACGGGTACGGTCA11.2195121951219512No Hit
AAAAAATACAGTGCAGATTCAAAAGGAGGAAAATGCTACAGCATTGGGAG11.2195121951219512No Hit
AGCCCAGAGCCACTCCTGCAGGAAGAGACAGGACAACCAGCGGTCACAAC11.2195121951219512No Hit
GTGCAGGCCAGCGGTGAACAGCGGCCCGTACACGCGCGTGGCGAATTGCT11.2195121951219512No Hit
TCCTCCGGGTAGCGCTTGCGTCGTTTCACCGGGTCATCGTCTTCGTCCAT11.2195121951219512No Hit
CAGCGTCAGATAGCGCTGGTGCAGGCGGGCGACATCCCCGGCGAGAGTAT11.2195121951219512No Hit
GGGCGAAACGGCGGTGATTGAAAACGACGTCTCAATCCTGCAGTCGGTCA11.2195121951219512No Hit
GTTCGCCACGGCCGGTAGCGCCGAAAAGTGTGACGCCTGCGTCGAGCTCG11.2195121951219512No Hit
ATGATGAGTTCTCTTGTTTTGTCTAAAAAAATTGACAATAAGAAGACCAT11.2195121951219512No Hit
CCACCAGGAAGGGCGGCTCCAGCAGGATCAACTCGCCCATCTGGCGCTTG11.2195121951219512No Hit
GTACGAAGGCCTGCTGGAGAAAAACGCCAACGAAACCAAATCCGGCGCCG11.2195121951219512No Hit
GTGAGTGGCTGGTTACCGAGCTGGCCGCACACCCGCGTATGGTGGTGCTG11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)