FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003677857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATGGCGGGTTTCACTCGGTTCGAGGCTTGCGAAGACAGTTTCATAAT42.941176470588235No Hit
CGAGCACCGCGGCGACGGCCGCGAGGCACCACGCGTCGGTGCCCGGTCGC42.941176470588235No Hit
GTACAGCCTCGGGTCGGTCGGGAAGTTCGGCGCGAGCGCGATGCTCATGA32.2058823529411766No Hit
AACCTATTTACTGTGGCTTCCATTCAGTTTCAGTATTCCTTTTAGGGCTA32.2058823529411766No Hit
CAAGTGCTCCAACCTCCTGGATTATACTGAAGTGAAGACTCATTATGGTT32.2058823529411766No Hit
CACTAATCTTAGTCCTCTTCCCAGTGATTGGGTAGGATTTGATCCAATTC21.4705882352941175No Hit
CCCATGAGTAATTCCTTCTTGGAAAATTGAAGCCGCTTCTGCGGCCTCGG21.4705882352941175No Hit
CTCCAAGATGTTTATCATTTACAGAGGGAGAAAAGAAACCTGGGAGATGC21.4705882352941175No Hit
CCCCTTACATCAGCATGACCTGGATGTGAGATCTGGAGTCAAAGGAGATC21.4705882352941175No Hit
GTTCATTTGTTACTCCTGAAAAGCAGAATGGGCACATGTGGGTTCTCAAG21.4705882352941175No Hit
CATTTGATTTATTAGAGAATTAGCGAATTGTTCTAGAATAAGATTATCTG21.4705882352941175No Hit
GTGTACGTGGACACCCAGGCGTCGACCTGCCAGCGCGGGGCGCCGTAGAC21.4705882352941175No Hit
GTCCTTCCGGTGTCTGTCGTCATGCCAGGCGGTGTGCTCCGGCATCGGGC21.4705882352941175No Hit
TTCCGGGACGGATTCCACGCCGATGATGTTGCCGCCGGTTTCGGCATAGA21.4705882352941175No Hit
TGGAATCATCGAATGGAATGGAATGGAACAGTCAATGAACTCGAATGGAA21.4705882352941175No Hit
GTCTAGTATGCAGCTGCTGCTACCTTCTCCTACCTGCCTGCTGTGCTGGA21.4705882352941175No Hit
TCGGTGACCAGGGCGCGCGCAATGGCCAGTTGCTGCTGCTGCCCGCCCGA21.4705882352941175No Hit
GCTCGGACCAGGTCGGCGATCAGCTCCACCGCCAGTGCCACGTCGGCCGG21.4705882352941175No Hit
GGATAGATCATTTGAAGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGT21.4705882352941175No Hit
GTCTCTGCCTTATTCATCACGTAGCCATCACTTTTGTATCTGTGTGTATC21.4705882352941175No Hit
TCGCAGTCTTTTTCTCCACGGGCCTGGACATCTGGTGGCTCGCCGGTGCG21.4705882352941175No Hit
GCTGAGAGCGGTGCTTGAAAACCGCAGTTAGTCGCCACAGGCTTCGCTGC21.4705882352941175No Hit
ACATGATCCAGGTGACGCACTCGGGCAACAGCTTCGTCTACCTCGCGGTC21.4705882352941175No Hit
TCCCAGGTTGCTCCGGGTGACTCTGGCCCAGCTTCTGGCTATCCATGAAC10.7352941176470588No Hit
GCCTAAGTCCCACGTAAGGCCAATTAAATTAGAATATTTGGGGAGAGGGG10.7352941176470588No Hit
GCGCGGGCACGCAGGCGAGCCAGGCGGAGGGCGAATTCGCCGACGCCGCC10.7352941176470588No Hit
CTACAAAAAGAGTGTTTCAAACCTGCTCTGTGAAAGGGAGTGTTCAATTC10.7352941176470588No Hit
GGATTCGGTGATCATGTCGGCGGCCGACGCCAACCAGGTGCCGGCGGGCG10.7352941176470588No Hit
GAACGAAAAAGGACAAAAGTAGCTATTCTTTGTGTATGTTTGGAGTCAAA10.7352941176470588No Hit
GGTCTGGGTGAGCGGGGTGATCGGCGGCTCGCCCTTCTTGGGGAGCGGCG10.7352941176470588No Hit
TTTTTGTTCAGGCCACCAGCCTTCAGGCCCCTTCGCCCAGGGGCTCGCTG10.7352941176470588No Hit
ATTTGGATGTATTTGAGGCCTTCGTTGGAAACGGGATTTCTTCATATAAT10.7352941176470588No Hit
TAATTCCATTCGATTCCATTCGATGATTCCATTCGATTCCATTCAATGAT10.7352941176470588No Hit
GAGCGCGACGACGATCAGGAAGAGCCAGTCGCGACCGGGGAACTCGAGCC10.7352941176470588No Hit
CTCGTAGGGGTGGCGCGCGGCGTCGCGGGCGCGATCGGCTTCCGATCGGC10.7352941176470588No Hit
GTCCCTCACCTCAGCGATCAATGTAATGTCCGGGTTCTGGCGGAGAACAC10.7352941176470588No Hit
GTGCGGGTGTCTCGGGCCTCGCGCTGCTGCGCGGGATGCCCGCCGAGTCC10.7352941176470588No Hit
TCATCACTGGTCACTAGAGAAACGCAAATCAAAACCACAATGAGATACCA10.7352941176470588No Hit
GCGTGCCTGCCGCGCCGCTGCGGCCCGCCCGCGCCTCGCGCCCGGCCTTG10.7352941176470588No Hit
GAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAAATCAGGTC10.7352941176470588No Hit
ACATAGCGAGATTCCCATCTTTACAAAAATTTTTTAAACTAGCCGGGTGT10.7352941176470588No Hit
GACCCCGAGGTCGAAGAGGACCCCGGTCACCGGCGGATCGCCGAGCTCGT10.7352941176470588No Hit
GGTTAGCCAGGGTCTCAAAGAAATACGAAAACCCAAAACATCAACAGGCA10.7352941176470588No Hit
GAAATGATTGGAATCATCATCGAATGGAATCGAATAGAATCTGTCTCTTA10.7352941176470588No Hit
GAAATCAAAATGCATCATGCTTCGCTGGCAGGATTCAATTCACTGACCTA10.7352941176470588No Hit
CTTCAACATCATTCTTATCAACAGTTATTTTAAACACGACACCTAAGTGG10.7352941176470588No Hit
ACCCAGACTGGTCCCTAACTCCTGGCCTCCAGTGATCCTCCTGCCTGGAC10.7352941176470588No Hit
GCCCGATGCGGCGGCTGTACCGCCTCCCGCCGAAGTCGTGCCTTCCTGGC10.7352941176470588No Hit
TAAAAAAACCCGCCGAAGCGGGTTTTTACGTTATTTGCGGATTAACGATT10.7352941176470588No Hit
GGTCGGCACGGTCGGCGTGAGGGTTGGGATCGTATCCGTACCCAACGATG10.7352941176470588No Hit
AACTTGTCCCACTCCCGCCGCTTCCACCAGCGGATGTCGGGGTGCTGTCT10.7352941176470588No Hit
GACATATGTTTGCATTACCCTTGGGTATATACCTAGGAATGGAAATGCTG10.7352941176470588No Hit
GTCTGGATTGGATGCTTCAGGAAAAGGTGGCGTAATGCGGATTGGTTACC10.7352941176470588No Hit
ACATGATCCAGGTGACGCACTCGGGCAACAGCTACGTCTACCTCGCGGTC10.7352941176470588No Hit
GGGCGGGGTTTCGGAGAGTTGTTTCAACGCACGGATCTCGATGATGATGA10.7352941176470588No Hit
AAGCAACATTGAACAATTGATCAAGGTGAGCCTGAGACCATGAGCGTTGG10.7352941176470588No Hit
CCTTTGTAACCGCGGCTTTGCTGGGGCAGCAGGCGCTTCCTGCCGACGCC10.7352941176470588No Hit
CCCTCCTCTGCTTACATGTTGTGATTAGTAGAAAAGGAAAACTTAAACTG10.7352941176470588No Hit
GGGTCTCAAACTCCTCTCCTCAACTGATCCACCCACCTTGGCATCCCAAA10.7352941176470588No Hit
CAGTGTTCCTCTCGAGGGAGACCGGGATTTCGGGGAACTTTGTGGGTCGC10.7352941176470588No Hit
AGTCACATCTTACATATATGGCAACAGGCAAAGAGAGAGCTTGTTCAGGG10.7352941176470588No Hit
GGCCTTCTCCAGCTACCAGCATCTGAAGCGGCAGGGCCGTGAGCCGGCCC10.7352941176470588No Hit
ATGTAGATGGGGCCGGCCTCGGCGGCGTCGGCCAGCGCGTCGGTGGACCA10.7352941176470588No Hit
GCACAAGTGTGAGCCCGGGTGAGTGTTGGTGAGAAGGCCAGTGTGGCCGC10.7352941176470588No Hit
GCTGGCGAACGACGCGAACGACAGCCGGCGCGCGCTGTCAGCGGGCGCGA10.7352941176470588No Hit
ATTCTCTCTCTCTCGTTTAAAACTAAAAACAAAAGAGACAGAGAGACTGG10.7352941176470588No Hit
GCCTCATGCTCCTGGACGGCTTGATTGCCGCAGCCGTCTTCATCGGCGTG10.7352941176470588No Hit
TTCCAAGCGGCATGCCTTCCTTCAGATGCTGGTCCCATGCCGGATCCTCC10.7352941176470588No Hit
GACCTGCGGCGGCAGCGAGCCGTCCGCGGGGACCGGGATCGTGCTCTCCG10.7352941176470588No Hit
CACTACTACTTCCGCGACAACTACCGCGCCGACTCCGGCATCATCGCCGC10.7352941176470588No Hit
CTTGAGAGATACTATTTAGGGTAACTGGAGGAAGAAATTTCTAAGCAGCA10.7352941176470588No Hit
ACCATGCTCCGGGGGTCGTTGGCGTCGACGTTGCTGGGCCAGAGGATGGT10.7352941176470588No Hit
CATGCTGCCCCTCCAGGACTCCGGGCTGCTTGATCAGCGCGAGCTCAAGA10.7352941176470588No Hit
GAGTTTCAGGTGCCCCCCAATTATCCCAGCCTCTTAGCACTCACAACCTT10.7352941176470588No Hit
CTGCAGCCCCAACCTCCTGGGCTCCAGTGATCCACCCACCGCCGCCTCCC10.7352941176470588No Hit
CTGCTCGAGCACGCGCGGGCCGGCCAGTGCGCCTTCCTTGGTCACGTGGC10.7352941176470588No Hit
CATGGTCCAATCGTACGGCGGTGGAGGTGTTCCGGGGGCAAGCTTCCTCA10.7352941176470588No Hit
GCAGATCGCTGCACAGCTTAAAACGCCTACAGCCCCTCCGCAGCGAGGTG10.7352941176470588No Hit
GTTGAATAAAATGCATCAAGCCTTAGTCAAAGATTTTGAACGCCTAAGTC10.7352941176470588No Hit
GTGATGTGTGCGCTCAACTCACAGAGTTTAAACTTTCTTTTAATATAGGA10.7352941176470588No Hit
ATGATAAGTTCCATTTACTTTCAGACAATTTCACTAGGCAGTGTCTGTCT10.7352941176470588No Hit
CCGCCACCCTTTTACTGATTACAACACCTGGGGTTCTGAGCGCCAGCCAC10.7352941176470588No Hit
CCTGGTAGACCCATCTAGGGAATTCGGAGTCTAGGAGGCCAGAAAGAGTC10.7352941176470588No Hit
GCCCAGGATGCCAGCTACCGGCAACGTGGAAATGGCGCCGCAGGTCATGC10.7352941176470588No Hit
CATTGGAAGGAGTAGCCGATGTTTTGCCAGTCCAGGCCGCGGGAGAAGCG10.7352941176470588No Hit
GAGGTGAGCTGACGCGAGACTGCGCAGGTGAAGCTGGTTGCAGGGGGTTG10.7352941176470588No Hit
GAGGCGGGAGAATGGCGTGAACCTGGGAGGCAGAACTTGCAGTGAGCTGA10.7352941176470588No Hit
TTGTATAACTTTAGGAGGCTATCTTCATTGAAAGTTTTTTCTCAAAAGCC10.7352941176470588No Hit
ACATTTTCCAACAACTACTTCCGTACGTTCACCAAGGACAACGTGCATCT10.7352941176470588No Hit
CCCTCGTGAGCCGGCCGGACCCAACACCTCCGCCGCCCTCCCCCAACACC10.7352941176470588No Hit
GGCCATGGGGACAGCAGGGGGACCGCTCGACGGCAAGGCAGCGCTCGTGA10.7352941176470588No Hit
GGACAGACAAGGCAAAATTAAAAGGCACTGTAAGGAAATGTCTAGAATAA10.7352941176470588No Hit
ACAGTGGCGCGATCTCAGCTCACTGCAAGCTCCGCCTCCTGGGTTCATGC10.7352941176470588No Hit
CCCACGAGCGGATCGCCGGGTGGGTGGACGCCGGCGTGCTCGACGGCATC10.7352941176470588No Hit
TACTTCACGTGCACTTGTATCGTCTGCAGGATCTGCATTACGCAATTGAC10.7352941176470588No Hit
GCTCGCACCTGTAATCCCAGCACTTTGGGAGGCTGAGGAGGGCAGATCAC10.7352941176470588No Hit
TTCCTGTTCACCCTGATGGACGCGACCGGCAAGCACCTGACGCAGGCCGG10.7352941176470588No Hit
GGCCGCAGTCCGAGCTGGACAAGGAGTATCTCGTCCGCGTCGACGCCCGC10.7352941176470588No Hit
TCTGTCTGCCTTGGCCTCCCAAAATGCTGGGATTACAGGCATGAGCCACC10.7352941176470588No Hit
CAACAATACTGACAGAAACTGATGTCAATAAACTGACGTGAAAAATGAGC10.7352941176470588No Hit
CCATTCTATTCCACTCGTGTTGATTCCATTCCTTTCCATTCCATTCGGTT10.7352941176470588No Hit
TCCTCGAGCGCGCCTGCCTCGATCGCACGCCCGAACAGGTTCTCGTCGAT10.7352941176470588No Hit
ATAATTGTAGTATCCATTTTGTCAAGCCGTACAATGGACTTGCATTAGAG10.7352941176470588No Hit
TCTCTCTTCCGCTGGATTTGCTAAGCTACCCCAAGGGGAGTTGGCCTGAG10.7352941176470588No Hit
GAGTACACACATCTCAAATAATTTTCTGAGAATGCTTCTGCCTAGTTGTT10.7352941176470588No Hit
GAGGCCACTGCTGCTGCCACGTGGGCCTCTGTCCTGCGCCTCTCCTGGGA10.7352941176470588No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)