FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003677859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003677859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTGCATAGCTCTGAGGATTTCGTGGGAAACGGGATTGTCTTCAGGTA39.67741935483871No Hit
AACGCAGGAACTTGTCCGGGTTCATCCAGGTCATCAGCGCAAACACAAGC26.451612903225806No Hit
AATCAGGACTGTGCTAGAAAATTCAGAAGTGTAGATCCCTAGAAGTTTCC26.451612903225806No Hit
ATACCGTGCTTGCCGTGTCGTCCATTGGGCGAAAGGCGAGGGTTCTGTGC26.451612903225806No Hit
CCTGCATCACGACCCGGGGCTGCAGCCCGAGCGCACCGTGATGAGCTGGG13.225806451612903No Hit
CATCTCACAGGTTTAAACGTCTCTTTGGATTCAGCAGTTTGGAAATACTG13.225806451612903No Hit
CCTGCGAGCCTAGGTTGTATGGACTCGGAATAGCGGCGCCTAGTCAGCGC13.225806451612903No Hit
ACCGCGCCGAGGGCGTCCACTTCTGGACGACCGACGGGACTGTCTCTTAT13.225806451612903No Hit
AATTAGCGGGGCGTGGTGGCGCAGGTCTGTATTCCCAGCTACTCTGGAGG13.225806451612903No Hit
ATTCATTCCTGATATATTTGTGCCTGTTTTTTTTTTTCTCTCAGTCAAAC13.225806451612903No Hit
CACCTGGGTGGATGAGAGGAGGGTACTCCTTGTTACTAATGCATGGGCTG13.225806451612903No Hit
GCCCAGCGCAATACCTGAGCTCAAACAGTTTGCCTGCCTTGCCCTCCCAA13.225806451612903No Hit
GATATCGTGGTGCATTCGCTGACCAAATACCTGGGCGGGCACGGCAACAG13.225806451612903No Hit
GTTTTCGGGTGCTGACATCCGGGGCCAGAAAAGCGATTCGCCGTCTCAGC13.225806451612903No Hit
CTTCCAGCGCACTCAGAAACCCATGCTGCATAAACGGCTGGGCCGTTGGC13.225806451612903No Hit
GGCTCGCCGAACGGGCTGTAGATACGTTTCCCGCCCTCAAGGATGTGAGG13.225806451612903No Hit
GGCTATGCAAGTACCACTGAAGAAGAGGGCATGCTCTGCACTCCCCGCCA13.225806451612903No Hit
GTGCTGCACCTGGAAGACCTGGAAGCGGCGGCGGGCGTGTCGCGGTTCAA13.225806451612903No Hit
ATATCACGTCACCCCAGTTAAAATGGCTTCTATCAAAAAGATGGGCAATA13.225806451612903No Hit
TCCCAGGGCGGGGCCTATCAGGACGCCCAGGCCAAGGCCATTCTGGAGCC13.225806451612903No Hit
GCTCAGGGCGTCGTCGCTGCCGAGACGATCGGCGGCGCGGAGACCCAGAC13.225806451612903No Hit
GTTCTACACGGTCATTGGTATCCTGCTTGCGATACTCATCGGGCTTCTAT13.225806451612903No Hit
GTTGAAAGATGCTTTACAAGAGAAAGAAAGACAAGCGGAGCAAGATATTT13.225806451612903No Hit
AAATAATAGTAACCTGAAGATGCTCTAACCACGTTAGAAATCTCTCCACG13.225806451612903No Hit
GACCCGCTGGTGAAGCGGCACGGTGGTCTTCATGCCCTCCACCACGAACT13.225806451612903No Hit
GTTCTTGTCTCACTCAAGAGGAAGTGGTGTTTTCGCCTTAATTGCATCTA13.225806451612903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)