FastQCFastQC Report
Tue 10 May 2022
EGAF00003678001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003678001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15073
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC670.4445034167053672No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC550.3648908644596298No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG520.3449877263981954No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA510.33835334704438397No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT480.3184502089829496No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT430.2852783122138924No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC380.25210641544483514No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG370.24547203609102367No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT350.23220327738340077No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA350.23220327738340077No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT340.22556889802958932No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC340.22556889802958932No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG330.21893451867577787No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA320.2123001393219664No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA310.205665759968155No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT310.205665759968155No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA290.19239700126053208No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG280.18576262190672063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.18576262190672063No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT270.17912824255290918No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG260.1724938631990977No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT260.1724938631990977No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT260.1724938631990977No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT250.16585948384528626No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT240.1592251044914748No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT240.1592251044914748No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA240.1592251044914748No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT230.15259072513766336No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG220.14595634578385192No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG220.14595634578385192No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA220.14595634578385192No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT220.14595634578385192No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC220.14595634578385192No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT220.14595634578385192No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT210.13932196643004047No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT210.13932196643004047No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC210.13932196643004047No Hit
GTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAGATGA210.13932196643004047No Hit
GTTCATAAACGAGGGGCGATGCCCGGGCTCAGAATCCAAGGAGCAAAACT200.13268758707622902No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA200.13268758707622902No Hit
GCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAG200.13268758707622902No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG200.13268758707622902No Hit
CAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACG200.13268758707622902No Hit
CGATATGACAAGTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACAT190.12605320772241757No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG190.12605320772241757No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC190.12605320772241757No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT190.12605320772241757No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC180.11941882836860611No Hit
ATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCA180.11941882836860611No Hit
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA180.11941882836860611No Hit
GCTGCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCC180.11941882836860611No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG180.11941882836860611No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA180.11941882836860611No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA180.11941882836860611No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG180.11941882836860611No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT180.11941882836860611No Hit
TCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCTGTCT180.11941882836860611Illumina Single End PCR Primer 1 (100% over 22bp)
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT180.11941882836860611No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA180.11941882836860611No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT170.11278444901479466No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC170.11278444901479466No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG170.11278444901479466No Hit
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC170.11278444901479466No Hit
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA170.11278444901479466No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC170.11278444901479466No Hit
CCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCC170.11278444901479466No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA170.11278444901479466No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG170.11278444901479466No Hit
GTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCA170.11278444901479466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG170.11278444901479466No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA160.1061500696609832No Hit
ACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGA160.1061500696609832No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG160.1061500696609832No Hit
CTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCG160.1061500696609832No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG160.1061500696609832No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC160.1061500696609832No Hit
GAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGC160.1061500696609832No Hit
CCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTG160.1061500696609832No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG160.1061500696609832No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA160.1061500696609832No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG160.1061500696609832No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC160.1061500696609832No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA160.1061500696609832No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC160.1061500696609832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)