FastQCFastQC Report
Tue 10 May 2022
EGAF00003678163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003678163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19386
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.3610853193025895No Hit
TGCCTGGACAGTGGCCCTGCAAGCAGCCAAGGATGGGGTCTCCACCACCT460.23728463839884453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.2321262766945218No Hit
GTACATGGGACACCCCGCGCAGGATTCGGACGGAGAGCGCGAGGACTCGG440.2269679149901991No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA390.20117610646858555No Hit
GGTTTACTGAGAGCATTAGGGGCAACAAGGGAGCCCTGGGCATCTGGGGA360.18570102135561745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.15990921283400392No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA270.1392757660167131No Hit
AGCACACCCTGAGCAAGAAGGAGCTGAAGGAGCTGATCCAGAAGGAGCTC260.1341174043123904No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA250.12895904260806768No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA240.12380068090374496No Hit
GAGATACGGAGGCCCGGTCAGACTCTCCCTCTGCACCTCAATGGAAACGA230.11864231919942227No Hit
CAACAAGACTATGCGCATTCGCATGACAGATGGACGGACACTGGTCGGCT220.11348395749509955No Hit
GTACATGGGAGAGCTGAGCGACCCAGCCCGCGAGCGAGATGCCGGTGGCC220.11348395749509955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG220.11348395749509955No Hit
CCCAGAGGGTGTCTCCATCTTCCCTATTTATTTTCAGCCCTTGAGGGCTT210.10832559579077684No Hit
GTACATGGGTGCTGCCGCCGGGGCAGCGGCGGGTCGAAGGAGACCCGGGA210.10832559579077684No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA200.10316723408645415Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGGAGAGCTCGGCGGCTGGGACTGGAGGACAGCGGTGGCGGAG200.10316723408645415No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)