FastQCFastQC Report
Tue 10 May 2022
EGAF00003678589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003678589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5820
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC380.6529209621993127No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT370.6357388316151202No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC300.5154639175257731No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC260.4467353951890034No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT250.429553264604811No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG250.429553264604811No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA240.4123711340206186No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA220.37800687285223367No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT200.3436426116838488No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT190.32646048109965636No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC180.30927835051546393No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC170.2920962199312715No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG160.27491408934707906No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA160.27491408934707906No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG160.27491408934707906No Hit
CTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGG150.25773195876288657No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG150.25773195876288657No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG150.25773195876288657No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA140.24054982817869416No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT140.24054982817869416No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG140.24054982817869416No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG140.24054982817869416No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT140.24054982817869416No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC140.24054982817869416No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC130.2233676975945017No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA130.2233676975945017No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA120.2061855670103093No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG120.2061855670103093No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC120.2061855670103093No Hit
GGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC120.2061855670103093No Hit
GGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTC120.2061855670103093No Hit
TGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGC120.2061855670103093No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC120.2061855670103093No Hit
CCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTG120.2061855670103093No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG110.18900343642611683No Hit
GTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCT110.18900343642611683No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT110.18900343642611683No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT110.18900343642611683No Hit
GTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCT110.18900343642611683No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC110.18900343642611683No Hit
CCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGA110.18900343642611683No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC100.1718213058419244No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG100.1718213058419244No Hit
GGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCATACACCACGAGGCCAGT100.1718213058419244No Hit
GTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGA100.1718213058419244No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC100.1718213058419244No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA100.1718213058419244No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT100.1718213058419244No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT100.1718213058419244No Hit
GTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCAT100.1718213058419244No Hit
TCCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACC100.1718213058419244No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC90.15463917525773196No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC90.15463917525773196No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGC90.15463917525773196No Hit
GGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACC90.15463917525773196No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG90.15463917525773196No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT90.15463917525773196No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA90.15463917525773196No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT90.15463917525773196No Hit
GACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAG90.15463917525773196No Hit
CCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAACTT90.15463917525773196No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC90.15463917525773196No Hit
GGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAG90.15463917525773196No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCT90.15463917525773196No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC80.13745704467353953No Hit
CACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAAC80.13745704467353953No Hit
GGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGG80.13745704467353953No Hit
GACCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGT80.13745704467353953No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT80.13745704467353953No Hit
GCTCTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCT80.13745704467353953No Hit
GGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAG80.13745704467353953No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCACTCCCA80.13745704467353953No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG80.13745704467353953No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG80.13745704467353953No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT80.13745704467353953No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT80.13745704467353953No Hit
CCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGG80.13745704467353953No Hit
GAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCG70.12027491408934708No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA70.12027491408934708No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC70.12027491408934708No Hit
GCCCAGCAACGCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTT70.12027491408934708No Hit
CTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGG70.12027491408934708No Hit
CACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTC70.12027491408934708No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT70.12027491408934708No Hit
ACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCTGCTGC70.12027491408934708No Hit
TTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAG70.12027491408934708No Hit
GGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCA70.12027491408934708No Hit
CTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAATGCTCATCTCCAG60.10309278350515465No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT60.10309278350515465No Hit
CTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAGAGCCTCAAGA60.10309278350515465No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA60.10309278350515465No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG60.10309278350515465No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG60.10309278350515465No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG60.10309278350515465No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG60.10309278350515465No Hit
CTGCCTGGCCCAGCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTG60.10309278350515465No Hit
CGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTG60.10309278350515465No Hit
GCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCG60.10309278350515465No Hit
GCTCCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTAC60.10309278350515465No Hit
GCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATC60.10309278350515465No Hit
GGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC60.10309278350515465No Hit
GTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTT60.10309278350515465No Hit
GCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGTAGTCAT60.10309278350515465No Hit
CCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCT60.10309278350515465No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA60.10309278350515465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)