FastQCFastQC Report
Tue 10 May 2022
EGAF00003678621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003678621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619116
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTGGAATTC17810.28766822372544076No Hit
ATCTTGGGCTGTGACAAAGTCACATGGTTCACACGGCAGGCATACTCATC11260.1818722178073253No Hit
GTACATGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTC10020.1618436609617584No Hit
GTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTACTCTCTCTTTCTGGCC7740.12501695966507084No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC7600.12275567098895845No Hit
GCTTACATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCAC7450.12033286169312375No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC7130.11516420186200971No Hit
ATTCAGTGTAGTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAGACAAG6690.1080572945942279No Hit
GGCCTTAGCTGTGCTCGCGCTACTCTCTCTTTCTGGCCTGGAGGCTATCC6550.10579600591811551No Hit
GTGTAAGTGTATAAGCATATCAATATTAAAAAGCAAGCAAGCAGAATTTG6450.10418079972089238No Hit
GTACAAGAGATAGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAATGCT6380.10305015538283617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)