FastQCFastQC Report
Tue 10 May 2022
EGAF00003678765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003678765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46419
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA3160.68075572502639No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT1870.4028522803162498No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG1700.36622934574204524No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC1470.3166806695534156No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG1450.3123720890152739No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT1320.2843663155173528No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT1240.267131993364786No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA1180.2542062517503608No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC1170.25205196148129No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT1150.24774338094314827No Hit
GCTTTAATGGATCCATAAAGGGAAGAATGTTGTCCAGAGGTAAAGGTGCC1130.24343480040500656No Hit
CATTAAAGCTTCTTCTGAAAACTCTGGGCATTTCTGTTGAGCACCTTGTG1110.23912621986686486No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA990.21327473663801463No Hit
AAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGG990.21327473663801463No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG990.21327473663801463No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC940.20250328529266035No Hit
ATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAG940.20250328529266035No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG900.1938861242163769No Hit
CCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAACTATCTTCCTG830.17880609233288094No Hit
GTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCA830.17880609233288094No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG820.17665180206381007No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA820.17665180206381007No Hit
CTACAAAGGCTGATGGTCACCAGCAGGAAGATAGTTACCAGCTTCATGAC810.1744975117947392No Hit
CTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGT720.15510889937310154No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC720.15510889937310154No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG710.15295460910403066No Hit
GTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAAC710.15295460910403066No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG710.15295460910403066No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC690.148646028565889No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC690.148646028565889No Hit
CTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTC670.14433744802774726No Hit
CTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCC660.1421831577586764No Hit
CCTTGTGGAGGGGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTT660.1421831577586764No Hit
GGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTA660.1421831577586764No Hit
ACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGG650.14002886748960555No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC640.1378745772205347No Hit
CATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGG640.1378745772205347No Hit
ATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAG640.1378745772205347No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT630.13572028695146382No Hit
CCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCCACAAGGTGC620.133565996682393No Hit
CCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAAT610.13141170641332214No Hit
CCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGA590.1271031258751804No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA590.1271031258751804No Hit
CTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCT590.1271031258751804No Hit
CAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCATAAAG590.1271031258751804No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA580.12494883560610957No Hit
GGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGAAAAAT580.12494883560610957No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT580.12494883560610957No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG560.12064025506796786No Hit
CACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCT560.12064025506796786No Hit
CCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTC550.11848596479889702No Hit
CTTCCATCCCCATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGT550.11848596479889702No Hit
GGTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACT540.11633167452982614No Hit
CCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAA520.11202309399168445No Hit
ATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTC520.11202309399168445No Hit
CTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTT520.11202309399168445No Hit
GGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTC520.11202309399168445No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA510.10986880372261358No Hit
CTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTG500.10771451345354273No Hit
GAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTTGTTGATGAGG500.10771451345354273No Hit
GCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTG490.10556022318447186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)