FastQCFastQC Report
Tue 10 May 2022
EGAF00003678766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003678766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46419
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA3100.667829983411965No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT1760.3791550873564704No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG1700.36622934574204524No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG1530.32960641116784073No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA1450.3123720890152739No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC1370.29513776686270704No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC1360.2929834765936362No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT1280.2757491544410694No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT1270.2735948641719986No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG1250.2692862836338568No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT1200.2585148322885025No Hit
CATTAAAGCTTCTTCTGAAAACTCTGGGCATTTCTGTTGAGCACCTTGTG1170.25205196148129No Hit
GCTTTAATGGATCCATAAAGGGAAGAATGTTGTCCAGAGGTAAAGGTGCC1150.24774338094314827No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG1060.2283547685215106No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA1040.2240461879833689No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA1030.22189189771429801No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG1020.21973760744522716No Hit
GTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCA970.20896615609987287No Hit
CTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCT960.20681186583080205No Hit
CCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAACTATCTTCCTG850.18311467287102262No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC850.18311467287102262No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG840.1809603826019518No Hit
ACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGG800.1723432215256684No Hit
GTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAAC760.16372606044938495No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA760.16372606044938495No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT760.16372606044938495No Hit
AAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGG740.15941747991124322No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG740.15941747991124322No Hit
CATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGG740.15941747991124322No Hit
GGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTA730.1572631896421724No Hit
CTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTC730.1572631896421724No Hit
ATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAG720.15510889937310154No Hit
CCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGA710.15295460910403066No Hit
CTACAAAGGCTGATGGTCACCAGCAGGAAGATAGTTACCAGCTTCATGAC710.15295460910403066No Hit
GGTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACT690.148646028565889No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC680.1464917382968181No Hit
CCTTGTGGAGGGGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTT680.1464917382968181No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC680.1464917382968181No Hit
AGATAACTGTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGC680.1464917382968181No Hit
CACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCT670.14433744802774726No Hit
CTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGT660.1421831577586764No Hit
ATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTC650.14002886748960555No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC650.14002886748960555No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT640.1378745772205347No Hit
ATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAG620.133565996682393No Hit
GGGAAGAATGTTGTCCAGAGGTAAAGGTGCCAACTTGTCAACAGGAAGGG610.13141170641332214No Hit
CAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCATAAAG610.13141170641332214No Hit
CTTCCATCCCCATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGT610.13141170641332214No Hit
ACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTCTGGGC600.12925741614425126No Hit
TCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGGAC600.12925741614425126No Hit
CTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCC590.1271031258751804No Hit
GAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTTGTTGATGAGG580.12494883560610957No Hit
GTCCAGAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTTGTTGA580.12494883560610957No Hit
CTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTT550.11848596479889702No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC540.11633167452982614No Hit
CCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAA530.1141773842607553No Hit
CCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCCACAAGGTGC520.11202309399168445No Hit
TGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCC520.11202309399168445No Hit
TTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAG500.10771451345354273No Hit
CTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTG500.10771451345354273No Hit
TCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGG490.10556022318447186No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA490.10556022318447186No Hit
GGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGAAAAAT490.10556022318447186No Hit
CCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTC490.10556022318447186No Hit
CCATCAGCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTG480.10340593291540102No Hit
GTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTGAAGAAACTGCTG480.10340593291540102No Hit
GTTGACAAGTTGGCACCTTTACCTCTGGACAACATTCTTCCCTTTATGGA480.10340593291540102No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG470.10125164264633017No Hit
GCTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTC470.10125164264633017No Hit
CCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAAT470.10125164264633017No Hit
TTTTTAAACTCCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAAC470.10125164264633017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)