FastQCFastQC Report
Tue 10 May 2022
EGAF00003678767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003678767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45458
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA2750.6049540234942145No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT1920.422367900039597No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG1920.422367900039597No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT1420.31237625940428526No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC1390.30577676096616657No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT1370.30137709534075413No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG1270.27937876721369176No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA1240.27277926877557307No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG1200.26397993752474813No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA1180.25958027189933564No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC1180.25958027189933564No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT1160.2551806062739232No Hit
AAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGG1010.22218311408332964No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA970.21338378283250475No Hit
GCTTTAATGGATCCATAAAGGGAAGAATGTTGTCCAGAGGTAAAGGTGCC950.20898411720709226No Hit
CATTAAAGCTTCTTCTGAAAACTCTGGGCATTTCTGTTGAGCACCTTGTG940.20678428439438604No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG910.20018478595626735No Hit
CTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGT900.1979849531435611No Hit
GTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCA870.19138545470544238No Hit
CTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCT860.18918562189273616No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC850.1869857890800299No Hit
GTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAAC780.17158695939108629No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA760.16718729376567382No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC760.16718729376567382No Hit
GGTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACT750.16498746095296757No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG750.16498746095296757No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG740.16278762814026132No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC730.1605877953275551No Hit
CATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGG710.15618812970214263No Hit
CTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCC700.1539882968894364No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT700.1539882968894364No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC700.1539882968894364No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT690.1517884640767302No Hit
CTACAAAGGCTGATGGTCACCAGCAGGAAGATAGTTACCAGCTTCATGAC690.1517884640767302No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC680.14958863126402394No Hit
ATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAG670.1473887984513177No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG660.14518896563861144No Hit
CCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGA660.14518896563861144No Hit
CCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAACTATCTTCCTG660.14518896563861144No Hit
CTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTC620.13638963438778653No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG580.1275903031369616No Hit
CCTTGTGGAGGGGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTT570.12539047032425535No Hit
CTTCCATCCCCATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGT570.12539047032425535No Hit
CTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTT560.12319063751154913No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG550.1209908046988429No Hit
CAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCATAAAG550.1209908046988429No Hit
TTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAG540.11879097188613665No Hit
ATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTC540.11879097188613665No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA530.11659113907343042No Hit
CACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCT520.11439130626072418No Hit
ACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTCTGGGC510.11219147344801794No Hit
CCATCAGCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTG510.11219147344801794No Hit
CTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTG500.10999164063531172No Hit
CCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAAT500.10999164063531172No Hit
CCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCCACAAGGTGC490.10779180782260547No Hit
GAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTTGTTGATGAGG490.10779180782260547No Hit
GGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTC490.10779180782260547No Hit
GGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTA460.10119230938448677No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA460.10119230938448677No Hit
ATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAG460.10119230938448677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)