FastQCFastQC Report
Tue 10 May 2022
EGAF00003678768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003678768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45458
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA3250.7149456641295262No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT1920.422367900039597No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG1710.3761714109727661No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG1600.35197325003299745No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC1520.33437458753134763No Hit
GCTTTAATGGATCCATAAAGGGAAGAATGTTGTCCAGAGGTAAAGGTGCC1420.31237625940428526No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA1410.31017642659157907No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT1380.3035769281534604No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT1370.30137709534075413No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC1340.2947775969026354No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG1240.27277926877557307No Hit
TCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAA1090.23978177658497954No Hit
CTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCA1040.22878261252144835No Hit
AAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGG1030.22658277970874213No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG990.2177834484579172No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT950.20898411720709226No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC940.20678428439438604No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC920.20238461876897354No Hit
CTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCT890.19578512033085485No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG880.1935852875181486No Hit
GGTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACT880.1935852875181486No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA860.18918562189273616No Hit
ATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTC840.1847859562673237No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC810.17818645782920498No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC800.17598662501649873No Hit
GTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAAC790.1737867922037925No Hit
GTGCTCAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCA780.17158695939108629No Hit
CCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAACTATCTTCCTG770.16938712657838004No Hit
CATTAAAGCTTCTTCTGAAAACTCTGGGCATTTCTGTTGAGCACCTTGTG770.16938712657838004No Hit
ATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAG760.16718729376567382No Hit
CCACTGGCTTGGTGGATTTTGCTAGATTTTTCTGATTTTTAAACTCCTGA750.16498746095296757No Hit
CTACAAAGGCTGATGGTCACCAGCAGGAAGATAGTTACCAGCTTCATGAC750.16498746095296757No Hit
GGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAAAACTC750.16498746095296757No Hit
CCTTGTGGAGGGGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTT730.1605877953275551No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA700.1539882968894364No Hit
CATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGG700.1539882968894364No Hit
CTTTATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTC650.14298913282590522No Hit
ACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGG640.140789300013199No Hit
ATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCCTT620.13638963438778653No Hit
CTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTCC590.12979013594966782No Hit
CTTCCATCCCCATCCACCTCCGCTCTTTATCTTGATGTCACACCAAGTGT590.12979013594966782No Hit
TTTTTAAACTCCTGAAAAATATCCCAGATAACTGTCATGAAGCTGGTAAC580.1275903031369616No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT570.12539047032425535No Hit
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG570.12539047032425535No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG570.12539047032425535No Hit
CTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTG570.12539047032425535No Hit
CACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCT570.12539047032425535No Hit
CTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGT570.12539047032425535No Hit
CCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTC560.12319063751154913No Hit
GGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTA560.12319063751154913No Hit
GTCCAGAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTTGTTGA550.1209908046988429No Hit
ATCTTGATGTCACACCAAGTGTGATAGCGCCTCCAGCAGTTTCTTCACAG550.1209908046988429No Hit
CCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCTTCTGAA540.11879097188613665No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA540.11879097188613665No Hit
TATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGG520.11439130626072418No Hit
GAGGTAAAGGTGCCAACTTGTCAACAGGAAGGGGCACTTTGTTGATGAGG510.11219147344801794No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT510.11219147344801794No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC510.11219147344801794No Hit
CTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTT500.10999164063531172No Hit
TCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGCACCTTTACCTCTGGAC500.10999164063531172No Hit
GCTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTCCTTAGCCCCTC490.10779180782260547No Hit
GTTTCTTCACAGCTTCAGAAGCCTCTGGTCCCAGCTCATTTACACACTTC480.10559197500989925No Hit
GTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTT480.10559197500989925No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG470.10339214219719302No Hit
CAACAGAAATGCCCAGAGTTTTCAGAAGAAGCTTTAATGGATCCATAAAG470.10339214219719302No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.10119230938448677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)