FastQCFastQC Report
Tue 10 May 2022
EGAF00003679009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838818
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT19830.23640408288806392No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG17290.20612337837290093No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC17170.20469279390761763No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC12400.14782706141260676No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA12000.14305844652832914No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC11870.14150864669093893No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC11170.13316357064345305No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT10850.12934867873603095No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA10680.12732201741021296No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC10230.1219573256654006No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG10210.12171889492118672No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT10200.12159967954907978No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10160.12112281806065202No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA10160.12112281806065202No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC9940.11850007987429931No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC9940.11850007987429931No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9580.11420832647844943No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA9250.11027421919892039No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT9120.10872441936153016No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA9120.10872441936153016No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT8860.10562481968674968No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG8720.10395580447725251No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA8550.10192914315143452No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT8460.10085620480247204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)