FastQCFastQC Report
Tue 10 May 2022
EGAF00003679038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511671
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG31800.6214931078759593No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT28900.5648160634470197No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA28450.5560213496563221No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT22930.448139527157099No Hit
CTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG22030.43055009957570395No Hit
GTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGT21080.41198348157312026No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG20780.4061203390459885No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG19550.38208145468474863No Hit
CTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC19080.3728958647255756No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA18740.366250969861493No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG18140.35452468480722965No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG17980.3513976754594261No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC17960.35100679929095063No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC17210.3363489429731214No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA17070.33361280979379326No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG16710.3265770387612353No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT16190.31641425838087367No Hit
GTACATGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGG14760.28846661233487925No Hit
GTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGG14510.2835806602289362No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT14440.2822125936392721No Hit
GCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCC14040.27439507026976323No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA13910.27185437517467276No Hit
GACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCC13570.2652094803105902No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA13400.2618870328785489No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC12790.2499653097400478No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG12730.24879268123462148No Hit
GACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCC12330.24097515786511256No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC12060.235698329590694No Hit
GTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACC12000.23452570108526768No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC12000.23452570108526768No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG11970.2339393868325545No Hit
GCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGC11920.23296219641136592No Hit
GCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTT11910.23276675832712818No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGG11760.22983518706356232No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG11700.228662558558136No Hit
GCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGA11520.22514467304185695No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC11260.22006328285167617No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC11250.21986784476743845No Hit
CCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGC11220.2192815305147253No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10730.20970506438707684No Hit
GTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCG10380.20286473143875655No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC9960.1946563319007722No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA9940.19426545573229673No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC9750.19055213213178No Hit
GACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGG9600.18762056086821416No Hit
GGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACT9470.18507986577312374No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA9380.18332092301498426No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT9280.181366542172607No Hit
ACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCT9250.18078022791989384No Hit
CACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGG9230.18038935175141838No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT9230.18038935175141838No Hit
GCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGG9200.17980303749870521No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9190.1796075994144675No Hit
GTACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA8920.17433077114004897No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA8690.16983569520258135No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA8380.16377711459121194No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA8340.16299536225426103No Hit
GGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGAC8330.1627999241700233No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC8270.16162729566459696No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT8150.15928203865374432No Hit
CCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAG8030.15693678164289163No Hit
GTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACG7800.15244170570542398No Hit
GGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAG7760.1516599533684731No Hit
GGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGC7500.14657856317829232No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC7460.1457968108413414No Hit
GACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGT7390.14442874425167734No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG7300.14266980149353783No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC7190.14051998256692289No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7070.13817472555607022No Hit
GGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGG6980.1364157827979307No Hit
GGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGAC6980.1364157827979307No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT6930.13543859237674208No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG6880.13446140195555348No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC6830.13348421153436485No Hit
GCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGACGAC6800.1328978972816517No Hit
GGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCG6740.13172526877622534No Hit
GGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACC6720.1313343926077499No Hit
GGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCC6720.1313343926077499No Hit
CGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGC6590.1287936975126595No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC6510.12723019283875772No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA6500.12703475475452No Hit
GCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACC6480.12664387858604453No Hit
GGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTC6450.12605756433333137No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG6300.12312599306976554No Hit
CTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAG6250.12214880264857693No Hit
ATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGG6230.12175792648010147No Hit
GTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGA6210.12136705031162602No Hit
ACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCG6210.12136705031162602No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6200.12117161222738831No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTG6170.12058529797467513No Hit
CGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGG6150.12019442180619967No Hit
GTACATGGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATG6140.11999898372196197No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT6040.11804460287958474No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC6010.11745828862687158No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA5970.11667653628992067No Hit
GTATCAACGCAGAGTACATGGGACTCTTCTGGTCCGCACAGACTCAGAGA5940.11609022203720751No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT5880.11491759353178116No Hit
GGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCG5830.11394040311059254No Hit
GAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGG5780.11296321268940393No Hit
ATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGAC5730.11198602226821532No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT5710.11159514609973986No Hit
GGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTC5710.11159514609973986No Hit
CTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGG5690.11120426993126442No Hit
TTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTT5620.10983620334160035No Hit
CCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCT5600.10944532717312493No Hit
GGCAGGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCA5600.10944532717312493No Hit
ACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGT5580.10905445100464947No Hit
GTACATGGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATG5450.10651375590955907No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA5420.1059274416568459No Hit
GAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGT5410.10573200357260819No Hit
CACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAA5370.1049502512356573No Hit
CACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACT5340.10436393698294412No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC5320.10397306081446868No Hit
GTATCAACGCAGAGTACATGGGACTCTTCTGGTCCCCACAGACTCAGAGA5250.10260499422480461No Hit
GAGTACATGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCAT5250.10260499422480461No Hit
CAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGG5240.10240955614056689No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC5180.10123692763514054No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT5180.10123692763514054No Hit
CCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG5150.10065061338242738No Hit
GGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCC5120.10006429912971422No Hit
GACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCG5120.10006429912971422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)