FastQCFastQC Report
Tue 10 May 2022
EGAF00003679234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1274429
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT82520.6475056672439187No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC62950.49394670083621767No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC59980.4706421464043898No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT59730.4686804835734278No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA50330.39492196112925865No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG48070.3771885291373627No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT45630.3580426999071741No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA45090.35380550819229634No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG42260.3315994849458071No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG40910.3210065056586126No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT37250.29228776181332977No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT36490.28632430680720544No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT36450.28601044075425147No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG36250.28444111048948195No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA35270.2767513921921111No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG34880.2736911981758105No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT34450.27031713810655594No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA32970.25870409414726125No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG31650.24834651439978217No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG31510.2472479832144435No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG31470.24693411716148958No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC30860.24214765985394243No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT30690.24081372912888832No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG30340.2380674011655416No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC29760.2335163433977099No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA29270.22967148424902448No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC28980.22739595536510862No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT27370.21476284673371368No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG27240.21374278206161348No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC26850.21068258804531284No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA25780.20228667112879573No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG25560.20056040783754922No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG25270.19828487895363336No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC25150.19734328079477165No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC24830.19483235237114033No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC24660.19349842164608622No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA24430.19169369184160123No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA24030.1885550313120621No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT23210.1821207772265069No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC22590.1772558534057213No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA21960.1723124630716972No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC21920.17199859701874332No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC21660.16995846767454287No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG20440.16038555305944857No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT20380.15991475398001773No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC20330.15952242141382533No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT20180.15834542371524818No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA20150.15811002417553274No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG19850.15575602877837838No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT19790.15528522969894754No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT19720.15473596410627818No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG19310.1515188370635006No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT19120.15002797331196951No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG19120.15002797331196951No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC18760.1472031788353843No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG18660.14641851370299955No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC18260.14327985317346043No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA17860.1401411926439213No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG17630.13833646283943632No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT17230.13519780230989722No Hit
CACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCAGTGCCTACGTCTA17150.1345700702039894No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT17040.13370693855836613No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC16640.13056827802882703No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGGCCAAGCCCGCA16500.12946974684348833No Hit
CTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGG16470.1292343473037729No Hit
GGGCACCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC16440.12899894776405746No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC16240.1274296174992879No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG16150.12672341888014163No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAA16110.1264095528271877No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC15990.12546795466832597No Hit
GATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCA15710.1232708922976486No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA15590.12232929413878686No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT15500.12162309551964057No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAA15420.12099536341373274No Hit
CCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGG15190.11919063360924775No Hit
GAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGAT15050.11809210242390906No Hit
GTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCAT15050.11809210242390906No Hit
GAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCT14930.11715050426504732No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT14890.11683663821209342No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA14850.11652277215913949No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG14820.11628737261942407No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG14770.11589504005323169No Hit
TGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTC14720.1155027074870393No Hit
CCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAG14500.11377644419579278No Hit
TAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAA14480.11361951116931582No Hit
CTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTG14440.11330564511636192No Hit
GTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACAT13990.10977465202063041No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC13890.10898998688824563No Hit
GTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCT13820.10844072129557629No Hit
GACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTAGTGAG13820.10844072129557629No Hit
GTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCT13630.10694985754404521No Hit
CTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTT13550.10632212543813739No Hit
GAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCG13310.10443892912041393No Hit
CCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGG13240.10388966352774458No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA13000.10200646721002112No Hit
CACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTG12980.10184953418354416No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTGCCCACTCCCA12850.10082946951144393No Hit
CCACTGACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCT12840.10075100299820547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)