FastQCFastQC Report
Wed 11 May 2022
EGAF00003679438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3791993
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA265020.6988936952151548Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT138470.3651641761996924Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG96750.2551428760548872Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG84830.2237082188706572Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT81300.2143991299561998No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG59350.1565140020036957No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG57980.1529011261360451Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT52580.13866059351902812Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG46830.12349706341757488No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC46380.1223103523661568Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA45830.12085992774775692No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG43690.1152164574143465No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC43600.11497911520406288Clontech SMART CDS Primer II A (96% over 26bp)
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG43320.11424071721651385No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT40740.10743690718838352No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT40530.10688310869772175No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG40260.1061710820668709No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA39880.10516897051234009No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC39430.103982259460922No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT39280.1035866891104493Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)