FastQCFastQC Report
Tue 10 May 2022
EGAF00003679461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663196
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTACTACTGTGCGGCAGATCATGGCAGTGCGACAACCTTCGTGGGTGT30060.45325966984119326No Hit
CTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGA29610.44647434544237297No Hit
GTAGTAATCACTCTCATCGTCTTGTTGGATGTTCTTAATGATCAGGGATC21440.3232830113571252No Hit
GGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACAAGACGATGAGAG17990.2712621909661699No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17060.257239187208608No Hit
ATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGATC16270.24532717326401243No Hit
GCTCTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTT15040.22678061990723708No Hit
GGTATCAGCAGAGACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGC14840.22376492017442806No Hit
GTAATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATACA13200.19903618236539422No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12210.18410846868798966No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12070.18199747887502338No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11790.17777549924909078No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11660.17581529442276492No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11650.17566450943612447No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11040.166466625251057No Hit
GTCTTGGGCTCAGGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACA10850.16360171050488845No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10820.1631493555449671No Hit
GTACATGGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATG10650.16058601077207946No Hit
GACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGCCCTAGTGGGATT10500.1583242359724727No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10410.15696717109270863No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10020.15108655661373108No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG9950.15003106170724795No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9830.14822164186756254No Hit
GTACATGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATGG9820.14807085688092206No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9670.14580908208131532No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9670.14580908208131532No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9660.14565829709467487No Hit
GCCATACACTGGGTCCGCCAGACTCCAAGCAGGGGGCTGGAATGGGTGGC9610.14490437216147262No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCCTGGTCCCTCCGCCAAAAATCAC9460.14264259736186588No Hit
CACCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATC9430.14219024240194453No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9390.14158710245538272No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9290.14007925258897821No Hit
GATCATGGCAGTGCGACAACCTTCGTGGGTGTGATTTTTGGCGGAGGGAC8910.1343494230966411No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8700.13118293837719167No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8700.13118293837719167No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8700.13118293837719167No Hit
CCTCACAGGGTCCCTCTCCCAGCTTTTACTGACTCAGCCACCTTCTGCAT8670.1307305834172703No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT8160.12304054909860736No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG8140.12273897912532644No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA8110.12228662416540509No Hit
GCCATGATCTGCCGCACAGTAGTAATCACTCTCATCGTCTTGTTGGATGT8090.1219850541921242No Hit
ACTCAGGCCTGAGCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCC8000.12062798931236016No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT7970.1201756343524388No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG7950.11987406437915789No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7880.11881856947267475No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG7870.11866778448603428No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC7780.11731071960627024No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7670.1156520847532253No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7630.1150489448066635No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7510.11323952496697809No Hit
CAGTAATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATA7270.1096206852876073No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7230.10901754534104549No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7200.10856519038112414No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG7070.10660498555479829No Hit
CCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATCAG6970.10509713568839377No Hit
ATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATACAGCG6970.10509713568839377No Hit
GGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGC6940.10464478072847244No Hit
ATGCCATACACTGGGTCCGCCAGACTCCAAGCAGGGGGCTGGAATGGGTG6800.10253379091550612No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6760.10193065096894433No Hit
TTACTACTGTGCGGCAGATCATGGCAGTGCGACAACCTTCGTGGGTGTGA6740.10162908099566342No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6740.10162908099566342No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6660.10042280110253983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)