FastQCFastQC Report
Tue 10 May 2022
EGAF00003679462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663196
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTACTACTGTGCGGCAGATCATGGCAGTGCGACAACCTTCGTGGGTGT30870.46547325375906967No Hit
CTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGA29890.4506963250683056No Hit
GTAGTAATCACTCTCATCGTCTTGTTGGATGTTCTTAATGATCAGGGATC20870.3146882671186195No Hit
GGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACAAGACGATGAGAG17730.26734178131351816No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17340.2614611668345406No Hit
GCTCTATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTT16890.25467584243572033No Hit
ATATTACTGTGTGAAGAGAAAGGGGTACTCTGCTCCGGAGGACTTCGATC16870.2543742724624395No Hit
GGTATCAGCAGAGACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGC14950.22542355502747302No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12780.19270321292649534No Hit
GTAATATAGAGCCGTGTCCTCAACTCTCAGGCTGTCCATTTGCAGATACA12480.18817966332728184No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11880.1791325641288548No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11780.17762471426245033No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11530.17385508959643906No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10980.1655619153312143No Hit
GACCAGGGAAGGGCCCCCGATTTGTGATGCGAGTGGGCCCTAGTGGGATT10900.1643556354380907No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10880.1640540654648098No Hit
GTACATGGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATG10620.1601336558121581No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10510.15847502095911314No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10460.1577210960259109No Hit
GTCTTGGGCTCAGGCCTGAGTCGATCCCTGATCATTAAGAACATCCAACA10440.15741952605263No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10120.15259440648013559No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10060.15168969656029288No Hit
GCCATACACTGGGTCCGCCAGACTCCAAGCAGGGGGCTGGAATGGGTGGC10030.15123734160037156No Hit
GTACATGGGGAGAGACTGAAGAACCCAGCATTGCAGCAGCTCCACCATGG9830.14822164186756254No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9830.14822164186756254No Hit
GCCTTGGGCTGACTCAGGACGGTCAGCCTGGTCCCTCCGCCAAAAATCAC9620.1450551571481131No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9520.14354730728170859No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9240.13932532765577596No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9120.13751590781609058No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8960.13510334802984336No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8950.1349525630432029No Hit
CACCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATC8940.13480177805656246No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG8730.13163529333711302No Hit
CCTCACAGGGTCCCTCTCCCAGCTTTTACTGACTCAGCCACCTTCTGCAT8680.13088136840391076No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8520.12846880861766355No Hit
GATCATGGCAGTGCGACAACCTTCGTGGGTGTGATTTTTGGCGGAGGGAC8350.1259054638447759No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8280.12484996893829275No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8130.122588194138686No Hit
ACTCAGGCCTGAGCCCAAGACTGAGAAGCGATCAGGGATGCCATCCCCCC8130.122588194138686No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT7950.11987406437915789No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7890.1189693544593152No Hit
GCCATGATCTGCCGCACAGTAGTAATCACTCTCATCGTCTTGTTGGATGT7760.11700914963298935No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7640.11519972979330395No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT7560.11399344990018034No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7480.11278717000705674No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7360.11097775016737134No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG7250.10931911531432638No Hit
CCTGCACCCTGAGCAGCGGCTACACTAATTCTAAAGTGGACTGGTATCAG7110.10720812550136008No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA6890.10389085579527017No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC6850.10328771584870837No Hit
ATTCTAAAGTGGACTGGTATCAGCAGAGACCAGGGAAGGGCCCCCGATTT6790.10238300592886568No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT6760.10193065096894433No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6710.10117672603574207No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTT6670.10057358608918027No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTG6670.10057358608918027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)