FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003679479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATGGCACCACCACACTCCAGCCTTGGCAACAGAGTGAGACCCTGTCT36.976744186046512No Hit
GGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGA24.651162790697675No Hit
CTGTAATTCAGTCTTTAACTGTGAACTTTTACATGATGGAAGCAGTGAAG24.651162790697675No Hit
CGCGAACTCCATCTCGTGCTGCGGATACCGCGAACCATCCGGCCGATGGT24.651162790697675No Hit
GCCTTGGAGTGAGTGGATACCTTGTTTCAAGGTTTACAGGAAGTATTTTT24.651162790697675No Hit
ACCTAAGTGCGTGCCGCCACCCGATGGAAGATTCGATGGACATGGACATG12.3255813953488373No Hit
GAATTCCTGGTCCGACTTAGAGACAGAGGTTCTAAAGAAAAAATAACTTT12.3255813953488373No Hit
CCTGGTGGAGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTAAGCAACCAA12.3255813953488373No Hit
GTCAGATGTGTATAAGAGACAGAACGCAGAGTACATGGGAAGCAGTGGTA12.3255813953488373No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12.3255813953488373No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAG12.3255813953488373No Hit
TCTTTGATAACGTCCACAAAGTGGGCGTCCGTTTTCTCCCGGATGTTCTT12.3255813953488373No Hit
GTCCCCAGCTCGCGCTCACTCAATCCTTAGAGTGGAAGAGATTGACTGAA12.3255813953488373No Hit
GCATGCTGCTAGCTCAATGGAGCACTAAGGGAAGCGGGAAGGGAGCAGAC12.3255813953488373No Hit
GGCCCGGTTCATCTCTGAAATCTCATTCCGGGTATTCCGGAGGTCGTCCC12.3255813953488373No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.3255813953488373Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGATTCCCTGT12.3255813953488373Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12.3255813953488373Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCAT12.3255813953488373No Hit
TTCCTGTTCCTGCGCACGCCGCAGCAGCGGGTCGATTCGTTTGGACTGCA12.3255813953488373No Hit
ACCCAGCACAATGAAGATCAAGATCATTGCTCCTCCTGAGCGCAAGTACT12.3255813953488373No Hit
ATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTG12.3255813953488373No Hit
GTGATTGATCACATGAGCCGCATCGCCATGTATGAGCTATTGGCAGATGC12.3255813953488373No Hit
CTCCCTGCGTCATGGATTTCAAGGTCTTTTAATCACCTTCGGTTTAATCT12.3255813953488373No Hit
GCTGAGAGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTG12.3255813953488373No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT12.3255813953488373No Hit
GGTACATATCCCAGCTTGTGGCCTGCGGCGATGTTAGTTAGGCAAGCATG12.3255813953488373No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA12.3255813953488373Clontech Universal Primer Mix Long (95% over 21bp)
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC12.3255813953488373No Hit
CTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAG12.3255813953488373No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT12.3255813953488373Clontech SMART CDS Primer II A (100% over 26bp)
GTCTTCCACAATGGTTGAACTAATTTACACTTTCACCAACAGTATAAAAA12.3255813953488373No Hit
GGGTGTTGGTGTTGGTCGGCGGCGGAGCGAGTCTCCTCTGCGCCCTTGCC12.3255813953488373No Hit
ATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATAC12.3255813953488373No Hit
GCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGA12.3255813953488373No Hit
GATTGGCAGTCAACCCGGCGAGCAGAGACGTGACGCCCGCCTCGGCGAGG12.3255813953488373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)