FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003679480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGGAGGTGGAGCTCAGGCAGTAATGCTCGCGAGCCGCCACTCATTTC36.976744186046512No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA24.651162790697675No Hit
GTATACCCAGGTACCTCCTCATTTTTCTTGTTGAATTTGAGATTTAAACC24.651162790697675No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA24.651162790697675No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG24.651162790697675Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACTAAATTAATTGACCTGAAAAAGCCTATCAAATACTATCAAAATGTA24.651162790697675No Hit
CTAATATGCACTGGCCCTGAACCACACTTCAACCTTAAGACCACAGGTGG12.3255813953488373No Hit
GTCTCACATACTCAGCTGAGAATTCTTCAATAGGTATGATACAAACTTGA12.3255813953488373No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA12.3255813953488373No Hit
GGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGA12.3255813953488373No Hit
CGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAG12.3255813953488373No Hit
ATGATAAGTAGGTGGGTGGGTGACCCACAGGGCTGGCATCAGAAGGAAAC12.3255813953488373No Hit
GTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATT12.3255813953488373No Hit
AATCTACTGTGACCAGCTCAGAATCATCTCTCTGTAGACCTAAAAACTAA12.3255813953488373No Hit
AATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGT12.3255813953488373No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12.3255813953488373Clontech SMART CDS Primer II A (96% over 26bp)
ATGATGGACACCTCGGTCTTGGACGCCGGGCGACGCAGCCGGGCGGCGTC12.3255813953488373No Hit
CCGCCGGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCT12.3255813953488373No Hit
AGTGCGGAAGGCTCGCGAGGTCGCTGCTCGCTACCTGGGCCCTCCCGAGG12.3255813953488373No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGT12.3255813953488373Clontech Universal Primer Mix Long (96% over 25bp)
CCATTTGACAAAGGGCTAATGTCCAGAATCTATAAAGAACTTAAATTTAC12.3255813953488373No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGGGGTTTAAACCAA12.3255813953488373No Hit
GACCAAGTTTGAGACCCTCCAGGCCCAGGCTGGGAAGCATGGGGACGACC12.3255813953488373No Hit
GACCTGTACGCCAACACAGTGCTGTCTGGCGGCACCACCATGTACCCTGG12.3255813953488373No Hit
GCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTC12.3255813953488373No Hit
GCCTACACCGCCAACCGCGACCTGATCGCCATCGGCGCCTACCAGCGCGG12.3255813953488373No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTA12.3255813953488373No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG12.3255813953488373Clontech Universal Primer Mix Long (96% over 26bp)
CCTGATATCGGGTCAGAGAGCCAAGGAGTCTCGCTGCAGCTGTGCAGAGG12.3255813953488373No Hit
ACTCTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT12.3255813953488373Clontech Universal Primer Mix Long (95% over 22bp)
AGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGG12.3255813953488373No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACCACGGGATCC12.3255813953488373No Hit
GCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTC12.3255813953488373No Hit
TTGCTAGCACCAAGCATTGCATCTGAAAGGGAAGCCAGTTATATTTATTA12.3255813953488373No Hit
GGGCGATACAGTGTCCAGGGAGGATTCCTCACTTTCTTCGAATCACTCAG12.3255813953488373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)