FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003679519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATCCATGAAGATATCTGGAACATTCGTAGTATCTGCAGCACCACCAAT34.3478260869565215No Hit
GTTCCACTGGGAGAACTAATTGATAATTGTGTTAAGATTGAAGATTAACC34.3478260869565215No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT34.3478260869565215No Hit
AAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGAC22.898550724637681No Hit
GATCAGTCGTGATCGGCGGGTTTTAGGTCGGGGTAGGTGTCGAGAATGGC22.898550724637681No Hit
GGTTAAGAACAGATCACACTAGGTAGGCCAAGAGCAAAATCTAGGAAGAA22.898550724637681No Hit
GATTGGGTCCCTGTGACCATCATTGATGTCAGAGGACATAGTTATTTGCA22.898550724637681No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG22.898550724637681No Hit
GCTAACTAGTTACGCGACCCGCTCGGGGGTCGCGTAACTAGTTAGCATGC22.898550724637681No Hit
CAAATTGTCTCAAAAGGACCAAGAGGAAAAGAGTCGGAGGGGTAGACCCT22.898550724637681No Hit
GTGTTGGACACTTACTGAAGCAGAGAAGATGTCCTTTGAAACTCAGAAAA22.898550724637681No Hit
GGAAAGGGAAGAGAGTGGCTCAGCCTGCAGGTAAATAGGCTAGAAAAGCC22.898550724637681No Hit
GAACCAAACCATATCACCAGGTAGTCCCAACATCATTCTCATGGGCTTCA11.4492753623188406No Hit
ATATGAAACATTGCATTTCATATTGCATAAAGGATGCCATTCTGATTAAC11.4492753623188406No Hit
CCCCACCCCTGCATCGGAGGGCAGGGCAGGCAGCAAGCAGCATTTGGAAT11.4492753623188406No Hit
CCCGCTAGGTGTTCAAAGCGTGGTTATACAACCCACTGCGGCTTGGGGGG11.4492753623188406No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT11.4492753623188406No Hit
TCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG11.4492753623188406No Hit
ACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT11.4492753623188406Clontech Universal Primer Mix Long (96% over 27bp)
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCG11.4492753623188406No Hit
AATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAA11.4492753623188406No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT11.4492753623188406No Hit
GTTCACAAAGGAGAAGCCAGCGAATGCAGACTGGTCCATGGAGTCGATGA11.4492753623188406No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC11.4492753623188406No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGTGTCCCATCC11.4492753623188406Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATACAGACCGTGAAACCGGGGCCTCACGATCCTTCTGACCTTTACTGTT11.4492753623188406No Hit
TCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCC11.4492753623188406No Hit
ATGTATGGCTGCGACGTGGGGCCGGACGGGCGCCTCCTCCGCGGGCATGA11.4492753623188406No Hit
GCCGATGAACGCCACCGTGTTGCCTTTCTGATCCTGAGTGTCGGCATGCA11.4492753623188406No Hit
GTACATGGGTGCAGTTTCTGACACTTGTTGTTGAACATGGCTAAATACAA11.4492753623188406No Hit
ATCTTAGCCTGACTTCACTAACCAGTATAAATCCAGTGGCCCCAGGAAGG11.4492753623188406No Hit
GTACGACGACTGCTTATATGGCTCAGTCCCTTGTGACACGCTTCCAGAAC11.4492753623188406No Hit
TCCTTACAGACACACCCAGAAATAATGTTTTACCAACTCTGTGGCCAGTC11.4492753623188406No Hit
GATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATG11.4492753623188406No Hit
AATTAAGAATGAATACATTTACAGGCGTAAATGCAAACCGCTTCCAACTC11.4492753623188406No Hit
CCCTGAGAGGGCCCGGCACCCCCCTTTCACGCCAGAACCCCGCACGCCAG11.4492753623188406No Hit
GATCAGATGTATTCTCAAGTGGAGGGCTTCTATGAAATAGCATAGCTCTA11.4492753623188406No Hit
TCGTCGCCGATTTCTACCCGTGTCCCCATGATCTACTCCTCAGTAAGCAT11.4492753623188406No Hit
GGTGCAGACATTGTATTACCAGGAATGGGTCCCTAGCTTGCACAACCCCA11.4492753623188406No Hit
CGGACATGCAGAGGTGGCACTCAGAGTCTCGCGGCAGCCATTCTCCTGTC11.4492753623188406No Hit
GGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGC11.4492753623188406No Hit
CTCAGACGCTGAAGTCTCCTGTACAGACAATGGCACATTCAATCTTTCAA11.4492753623188406No Hit
TGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGAC11.4492753623188406No Hit
ATTTCCAACATCTGGACCACATTGGAAGAAGACTGGACCACTATGAGAAA11.4492753623188406No Hit
CACCTAACTGTATGGCATAGTTATGCAGAAGTGCGCTGCTTAAGAGCGAT11.4492753623188406No Hit
CTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAAT11.4492753623188406No Hit
CAGACGAACCCCGGCGACACGAGGCCCTGGAGCGGGCGGTTGCCGGTCAT11.4492753623188406No Hit
TAATGAAATTTAAGCAGGGTCCAGGTGGAATAGGATCCTAGAATCCTAGA11.4492753623188406No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT11.4492753623188406No Hit
GGTGAGATCACGCCACTCATTGCATTGAGCCGAGACTGTACGCCAGCCTG11.4492753623188406No Hit
GTCGAGAAATATTGCTCAGCTTCAAGCTGATCTTGTAGCTCCCGCATCTG11.4492753623188406No Hit
AAGGAATGCTTTGGCTATTTGGGATCTTTTTTTTGTTTCATATGAATTTT11.4492753623188406No Hit
ACACATAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAATTATAA11.4492753623188406No Hit
TCGTAAGCGTCCTGCTGGTACCCGCTTCCTCCGCGGGTACCAGCAGGAAG11.4492753623188406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)