FastQCFastQC Report
Tue 10 May 2022
EGAF00003679521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303558
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTA6760.2226922037963091No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6450.21247998735002865Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA6400.21083285566514473No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT6260.2062208869474697No Hit
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA6230.20523260793653933No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA5880.1937026861423517No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5830.19205555445746778Clontech Universal Primer Mix Long (96% over 26bp)
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC4500.1482418516395549No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTT4300.1416533249000191No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4120.1357236508344369No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC4070.134076519149553No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC4010.13209996112769223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.12847627142094756No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT3660.12057003933350463No Hit
TAGATAGACAGATGGGAAAGGCAGGCGCGGCCTTCGTTGTCAGTAGTTCT3610.1189229076486207No Hit
CTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGT3440.11332265992001528No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT3340.1100283965502474No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3170.104428148821642No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA3070.1011338854518741No Hit
CATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAGTTAAACAGCTAA3040.10014560644094374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)