FastQCFastQC Report
Tue 10 May 2022
EGAF00003679561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758879
Sequences flagged as poor quality0
Sequence length140
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT44190.5823062701695527No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC34080.449083450721393No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT30690.4044122976126629No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG29740.391893832877178No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC29110.38359211415785655No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT25520.33628549478902436No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA25510.33615372147601924No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG22820.30070670027764634No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT22420.29543576775744224No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG21600.2846303560910237No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT19360.25511313397788055No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA19250.2536636275348244No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG19250.2536636275348244No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG18860.24852446832762534No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA18340.24167225605135995No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG18320.24140870942534975No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG18130.2389050164782528No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT18010.23732373672219154No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA17430.22968088456789554No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG16680.21979788609251277No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC16520.2176895130844311No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT16430.21650355326738516No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG16340.21531759345033924No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT16050.21149616737319127No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT15900.2095195676781147No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC15450.20358976859288502No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG15040.19818706275967576No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG14960.19713287625563497No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA13770.18145185200802763No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT13490.17776219924388473No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA13420.176839786052849No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC13350.17591737286181328No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG12990.17117353359362955No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG12880.16972402715057341No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA12850.1693287072115581No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC12700.16735210751648155No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG12620.16629792101244073No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT12440.16392600137834884No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC12370.16300358818731314No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC11960.15760088235410388No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC11570.15246172314690484No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG11160.14705901731369558No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG10890.1435011378625578No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC10780.14205163141950164No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG10610.1398114850984149No Hit
CACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC10490.13823020534235367No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC10440.13757133877732813No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGC10260.13519941914323627No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC10250.13506764583023118No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT10210.13454055257821076No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA10200.13440877926520564No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC10070.1326957261961393No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCA10010.13190508631810868No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA9980.13150976637909337No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT9800.1291378467450015No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT9530.1255799672938637No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCAC9460.12465755410282799No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG9450.12452578078982288No Hit
GTCTTGAGGCTCTCACTAGAAAAGTCCACAACTTCCAGATGGAATGCTCT9290.12241740778174122No Hit
CCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGG9240.12175854121671571No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC9220.12149499459070551No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA9160.12070435471267488No Hit
GGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCA8750.11530164887946565No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG8720.11490632894045033No Hit
GGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACC8720.11490632894045033No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT8690.11451100900143502No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC8680.1143792356884299No Hit
CCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGA8440.11121667617630743No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8350.1100307163592615No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT8270.10897652985522065No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC8180.10779057003817473No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG8140.1072634767861543No Hit
GCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAA8120.10699993016014411No Hit
CCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGC8070.10634106359511859No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAA7880.1038373706480216No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT7860.10357382402201143No Hit
GAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCG7800.10278318414398079No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT7770.10238786420496547No Hit
GCCTGGAAGTTGTGGACTTTTCTAGTGAGAGCCTCAAGACTGGGGATGCT7750.10212431757895526No Hit
GAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGAT7680.10120190438791955No Hit
GTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCAT7650.10080658444890424No Hit
GCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCG7620.10041126450988894No Hit
GTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCT7600.10014771788387873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)