FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003679831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length146
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGTGTTGATACCACTGCCCCATGTACTCTGCGTTGCTGTCTCTTATAC16.25No Hit
TTTTGGGTTGATACCACTGCTCCCATGTACTCTGCGTTGATACCTGTCTC16.25No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGATATCAACTCAGAGTACATGG16.25No Hit
TTTTTGCGTTGATACCACTGCCCCATGTACTCTGCGTTGATACCTGTCTC16.25No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAACTGTCTCTTATA16.25No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTAT16.25Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCACAGATTGTATATCGCGTATGCCGTC16.25No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATACA16.25No Hit
ACACATCTCCGAGCCCACGAGACCACAGATTGTATATCTCGTATGCCGTC16.25No Hit
GTCTTCTGCTTGCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATC16.25No Hit
GCCCATCGATGTGCCGCGGAACGTGTCCGTGAAGCGGGTGAGCATCGTGG16.25No Hit
TGTTTCTGGCTGCAGATGAAGAGGTCCCCGTGAATCAACCTGTCGCCCAT16.25No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCTC16.25No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA16.25No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16.25No Hit
ACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGTCTCTTA16.25Illumina Single End PCR Primer 1 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)