FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003679851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA292.843137254901961Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT151.4705882352941175Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG121.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC100.9803921568627451Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT40.39215686274509803Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT40.39215686274509803Clontech SMART CDS Primer II A (100% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC40.39215686274509803Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG40.39215686274509803Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAAC40.39215686274509803No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.39215686274509803Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40.39215686274509803Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA40.39215686274509803Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.29411764705882354Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30.29411764705882354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTCCCCGCCTCCCCTGGTGCCTAGCGTCAGGTCTAGCCACTTCCTCCT30.29411764705882354No Hit
TCTCAGCACTTTGGAAGTCAAGGCAGGTAGATCAGTTGAGGTCAGGAGTT20.19607843137254902No Hit
ATGCGATATCTAAATTCCGGTTCCCGGGGCGCCGAGTGTTGCTCGTCGTC20.19607843137254902No Hit
GACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTG20.19607843137254902No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902Clontech Universal Primer Mix Long (95% over 22bp)
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC20.19607843137254902No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.19607843137254902Clontech Universal Primer Mix Long (100% over 23bp)
CCCTACACGACGCTCTTCCGATCTCTGTCCCAGTTCTGCAGGTACCAGAG20.19607843137254902Illumina Single End PCR Primer 1 (100% over 24bp)
GGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGG20.19607843137254902No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCTCCTA20.19607843137254902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902Clontech Universal Primer Mix Long (95% over 21bp)
GGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTT20.19607843137254902No Hit
GTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGA20.19607843137254902No Hit
TTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAG20.19607843137254902No Hit
ATCACCACTCTGTTCGCAGCAGTCTGCGCCCTTACACAAAATGACATCAA20.19607843137254902No Hit
CTGGACCGCAGCACCTATCTGAGCAGTTAGAGCGTCTTTCTTTTCAGATT20.19607843137254902No Hit
GTACATGGGAATCCGGATCTACATCTTTCCCTACACGACGCTCTTCCGAT20.19607843137254902Illumina Single End PCR Primer 1 (96% over 28bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGC20.19607843137254902No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC20.19607843137254902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACG20.19607843137254902No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG20.19607843137254902No Hit
ATCCCAGGGCGGCCGAGCGGGCCAAGGCCCTGGTGGTGACGGCGGCCTAT20.19607843137254902No Hit
TACTCCAGCCTGGGCAACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAA20.19607843137254902No Hit
TCTATAAAGTCTAGATGCACAGGACACATGGCTATGACCAAATACCAGGA20.19607843137254902No Hit
GTTTATCTGATGCCATTCCAGGATTATACGTAGTTGAAAAAAGAAAAACA20.19607843137254902No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA20.19607843137254902No Hit
GAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGC20.19607843137254902No Hit
GGCGCATCCTGACCGCGAGCGTCGGGTCCCAGAGCCGGGCGCGGCTGGGG20.19607843137254902No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.19607843137254902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.19607843137254902No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC20.19607843137254902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCTGCTTGCAGCCCTGCGCATCATCGACAACGGCGATATCGATGCGGC20.19607843137254902No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC20.19607843137254902Clontech Universal Primer Mix Long (96% over 25bp)
GGCTAAGTGCTTGAACAGGGTCTCTTCTGTGGATGGAGGGGCATGCACAG20.19607843137254902No Hit
GATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAGAAAAAA20.19607843137254902No Hit
GAACACCTCAATGTACCGGTGTCCCATGCTTTCCCTGTCTTTTTTCAGGG20.19607843137254902No Hit
CCATTAGATTCCATCTGATGATGATTCCATTCGATTCCATTTGATGATGA20.19607843137254902No Hit
GTAACAGGACTTCTGGAAAGGTGTAAAGCAGAACCATCATTTTATCCCCG20.19607843137254902No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.19607843137254902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTCCCCAGCCCGCTCCGGACACTGCGCTCCAACCAGGAGGAGCAGGAG20.19607843137254902No Hit
CTCTTCGCCGCATACGTGCTGATAGAGTCCTCTGCAGTACATTTCCTGAG20.19607843137254902No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTATACTAGATGTGTCTTGATGAGGGCAGATCACTTGAGGTCAGGAG20.19607843137254902No Hit
GTCAAGGACTGGATGCAGAAGAAGCGAAGAGGTCTTCGCAACAGCCGGGC20.19607843137254902No Hit
CCTTATCATCGTCTCATCAACTTCTCACGACCTGTCTCTTATACACATCT20.19607843137254902No Hit
AAAACATACTGACACTGTTTGGAAATGGCAACAGGAAGATAGCAAAATGA20.19607843137254902No Hit
AAATAACAAACTAAACCAAAACCACGCAAATACTATAAAATCATTCAAAT20.19607843137254902No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
GGAGGGTAACCTGGTGCCACTTTTATGAAGGGCAATTTGGCATTGTGTAT20.19607843137254902No Hit
TGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGC20.19607843137254902No Hit
GGCTTGGAAAGATTGTAAGTAAAATAGATGGCGACAAGGACGGGTTTGTC20.19607843137254902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19607843137254902No Hit
CAGACGCTCCTGGAAGTTGAATACCCTCTGATTGAAGATGAGCTGAGGGC20.19607843137254902No Hit
GTCTAAAAGAGGAGAGGCGGGAGAAACCCTCAGTTCTGGGAATTCCCAGC20.19607843137254902No Hit
GTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAG20.19607843137254902No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)