FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003679852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003679852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA191.8627450980392157Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT161.5686274509803921Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG111.0784313725490196Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100.9803921568627451Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA80.7843137254901961Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC60.5882352941176471Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT60.5882352941176471Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT50.49019607843137253Clontech Universal Primer Mix Long (100% over 23bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50.49019607843137253Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGAT40.39215686274509803No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40.39215686274509803Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC30.29411764705882354No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTG30.29411764705882354No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA30.29411764705882354No Hit
GGTTGTTGCTTGTCCCTGACTCTCCATTTCCCCATCTGAGTGGATTTGGA30.29411764705882354No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC30.29411764705882354Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.29411764705882354Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT30.29411764705882354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.29411764705882354Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.29411764705882354Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTGGCGCACGCTGGTCTCGGCGCGTGCATGGTCAAAGCCTGCCGGCA20.19607843137254902No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19607843137254902Clontech SMART CDS Primer II A (100% over 21bp)
GGTAGCTGGCTATTTTTATGGTTATTTCTTGATCACACACTAAACAAGAA20.19607843137254902No Hit
GCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGG20.19607843137254902No Hit
ACCTTACTGCATCATCTACCCTTGGCTATCAGTGGATACCAATGCTCCAC20.19607843137254902No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT20.19607843137254902Clontech SMART CDS Primer II A (100% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
TTCCACATCTGTGATTCTCTCCAGTCAAAAGTTCTTTGAGACGATGCCAT20.19607843137254902No Hit
GAATGTAATCATCATCAAATGGAATCAAAGATAACCATCATCAATTTGTA20.19607843137254902No Hit
GTGGAGAAGATCCGGAGCTCTCCCGCCCCGTCAATACGGGCGGCTTCGAG20.19607843137254902No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA20.19607843137254902No Hit
GCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAG20.19607843137254902No Hit
CTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGCGTGTAGGAG20.19607843137254902No Hit
GTGTAGGGAAGGTGTAGACTCGGTCGGGGAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTACATGGGAACGCACCCCGATCTACACTCTTTCCCTACACGACGCTCTT20.19607843137254902Illumina Single End PCR Primer 1 (100% over 31bp)
GTACATGGGAAGCAGTGGTATCAACGCAAGTACATGGGAAGCAGTGGTAT20.19607843137254902No Hit
CTCCCCGTAAGGCTTACCTATCAGAATGTGAGGTATTTCTTTGTTGAAAG20.19607843137254902No Hit
CCTGAGCGGTGACCTGGCGGGTCGCGCCTGCGCTCTGCCCTGTTTCCTGC20.19607843137254902No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.19607843137254902No Hit
TTATATGGCTAACCCATTGCTGAGTAACTCAAGCTGTACTCAAAGACACG20.19607843137254902No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG20.19607843137254902No Hit
CTCTGTGCTCCAACTCGGACGTGGCCGCCCTCACCCTCTCGATGTAGCCC20.19607843137254902No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT20.19607843137254902No Hit
GGATAACACCGAGATTACACTCTTTCCCAGCAACTCCAGTCCTTGCATCA20.19607843137254902No Hit
CCCTGCCACCACGCTCCGCCCCCAGGGTTCGAACCCGGGCCTCAAGGCTC20.19607843137254902No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.19607843137254902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC20.19607843137254902No Hit
CCGTCCCCCCGGATCACGGGTCCGGCCACATCGCTCATCCAGTCCAGAAG20.19607843137254902No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
AAATGATACTTGCTTATCTGTGGCAACTTTTCTGCCGTATAGCAGTTTAA20.19607843137254902No Hit
GCGTTGATACCACTGCTTCCCATGTATCTGCGTTGATACCACTGCTTCCC20.19607843137254902Clontech Universal Primer Mix Long (95% over 24bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTCT20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
TCTCTAACCATCATCTGTTTATAGTTAAATCCATCATCAGGATCTGGATC20.19607843137254902No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT20.19607843137254902No Hit
CAAATAGGAATGCAGATGATGTGCCAAAACTCAGTAACTGGAACATATAT20.19607843137254902No Hit
TTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGC20.19607843137254902No Hit
GGTGTACGACACGAGATTACACTTTTCCTACACGACGCTCTTCCGATCTC20.19607843137254902Illumina Single End PCR Primer 1 (100% over 23bp)
GTTGAGGACGTGCAGAACTTCCTCTCTGACTGCACGATTCATGATGGGGC20.19607843137254902No Hit
GCAGGGCTGGAGGTCCTGGAGGGTGGCGGCGGGTCGTCCTAACTAGCAGG20.19607843137254902No Hit
ATGCTAGGCTGCCAATGGTGAGGGAGGTTGAAGTGAGAGGTATGGTTTTG20.19607843137254902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTCTACAACTCAGGTGAGTTT20.19607843137254902No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
GGCCCACAGCGAGAAGGGCTGCTGGGCCTGGTTCCTGGTCAGGAAGCCGC20.19607843137254902No Hit
GTCGTGAGAAGTTGATGAGACGATGATAAGGCTGTCTCTTATACACATCT20.19607843137254902No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA20.19607843137254902No Hit
AGACAGAGTTGTAACAACTGTACATCTTTCTTTTTTAAGGCAATCTCCAT20.19607843137254902No Hit
CTAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.19607843137254902Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTGCATCGCGTCGCCATGATGGGCCATCGTCCAGTGCTCGTGCTCAG20.19607843137254902No Hit
GTGTAGTGGCATGATCTCAGTTCAGTGCAACCTCCACCTCCCGAGTTCAA20.19607843137254902No Hit
GAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGC20.19607843137254902No Hit
TACTAGATGTGTCTTTTTTTTTTTTTAGACGAAGACTTGCTCTGTCACCT20.19607843137254902No Hit
CCGCTGTATGTGGGCCTGATCCTCTATGTGCTGGCGTCGGTGGCCTGCGC20.19607843137254902No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)