FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG32.4390243902439024No Hit
ACACCAGCCAGAGGGGAATCACTCATCCCAGTGGTTGGAACTGGAGTTTC21.6260162601626018No Hit
GTGCTGGCGCGGAAGGGTTCGCTGATGAACGGCCGGCGCGTGGTCAAGGC21.6260162601626018No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21.6260162601626018Clontech Universal Primer Mix Long (96% over 26bp)
GAGGCAAGAGCGTCGTGTAGGGAAGGTGAATCTCGCGTTCCATGTACTCT21.6260162601626018Illumina Single End PCR Primer 1 (95% over 21bp)
GTACATGGGTAGCGCACCGAGATCTACACTCTTTCCTACACGACGCTCTT10.8130081300813009Illumina Single End PCR Primer 1 (95% over 22bp)
TCCTTTCTCTGTGTTTCTGGAACAGTGATGTCGTGTTTACTCCTGTCTCT10.8130081300813009No Hit
ATGATACGGCGACCACCGAGATTACACTCTTTCCCTACACGACGCTTGCC10.8130081300813009Illumina Single End PCR Primer 1 (96% over 25bp)
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT10.8130081300813009No Hit
CCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG10.8130081300813009No Hit
GCCGTCGTACTGACCGATCTGGATGCCCGGATTGAGCGTCAGGAAGAACG10.8130081300813009No Hit
GAGCCCAACGCCAAGGCACTGGTCGAGGATGACGCCACCGGCAAGCTGTC10.8130081300813009No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACCTG10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
TTTGGAAAATCTGGATTGGGTTTTAATCCACAAAACAAGCAAGATAGATT10.8130081300813009No Hit
TAGCAGAGCAGCTCCCTCGCTGCGATCTATTGTAAGTCAAACCCTTGTGT10.8130081300813009No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGC10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGATCTCGGTGGCGCGTCACCCATGTACTCTGCGTTGATACCACTG10.8130081300813009Clontech SMART CDS Primer II A (95% over 23bp)
ATCTCGGTGTCGCCGTCACCCATGTACTCTGCGTTGATACTGTCTCTTAT10.8130081300813009No Hit
GGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGC10.8130081300813009No Hit
CTACCACAGGAATTACTAGGAAATCGCTGAACTGAGCTTGGAGGCACTAG10.8130081300813009No Hit
GCTGCAGGGGGATGTGCTCCAGCGGAAGGGTGGCACGTCGTCCCACGTCG10.8130081300813009No Hit
CTCTTAGACCATCCGTTCAATCTCTTCATCGGACAAGCCGCTCGAAGCGG10.8130081300813009No Hit
GAATGGTGGCCCCCTTCTCATAGCTCCATTAGGCAATGCCCCAGTAGGGA10.8130081300813009No Hit
CCTTTGGAGGAAACTCCAAAGGTGTAGCCCGTCAAACTCGGTGAAGGCCC10.8130081300813009No Hit
CTCCGCTGGCTGCTTCCTGTAAGAACGTAGACGATCTGATGCAGCATTAT10.8130081300813009No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTAAATTTAAAAAA10.8130081300813009No Hit
GGGTAGCCTGGACGGTGCCGTCGCCGCCGTTGATCACCAGCACCTTGGGC10.8130081300813009No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
TTTCAGCATTCTTGTCCATTGTGTGGATAAGAAATTGGATAAACAGTATG10.8130081300813009No Hit
GAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAG10.8130081300813009Illumina Single End PCR Primer 1 (100% over 40bp)
GGATGTAGGAGAGCTTCTTCATTCCCTGGAAAGCCCCATTTTCAATTCCT10.8130081300813009No Hit
ATCTGAGGCAAGAGCGTCGTGTAGGGAAGAGTGTAGATCTCGGTGGCGCC10.8130081300813009No Hit
CCGCATCTCCATTCCGTTCCCAGCGATGTCGGCTCCGTCCAGGCTTCATA10.8130081300813009No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.8130081300813009Clontech Universal Primer Mix Long (95% over 22bp)
ATCTTCAACTGTATGGGCTGCTGATAATGCACCGGTAGCGGCACAACAGC10.8130081300813009No Hit
ACCTAATTCTGAATCCTTGTGTTAGCTAGAAGCTTATAATCCGATTCACC10.8130081300813009No Hit
CTACACGACGCTCTTCCGATCTCATGATACAGCAACTCCAGTCCTTGCAT10.8130081300813009Illumina Single End PCR Primer 1 (100% over 22bp)
GCCGTCACTGGCCCCGCTAAGCCTCATCACCACACGGCCAGGCCAGACCA10.8130081300813009No Hit
GCTGAACGGTGAATCCGTTAGCGGTATCGGTCTGGCGCTGGTAGTGGCGG10.8130081300813009No Hit
GTCCCAGGTCTTGTCGATACCAGAACAGATCGGAAGAGCGTCGTGTAGCT10.8130081300813009Illumina Single End PCR Primer 1 (95% over 22bp)
GGTGGTGCCGCTGCGGGCGGTGCTGCGGCTGCTGAAGAGGAGAAGCCTGC10.8130081300813009No Hit
GTGTAGCTCGTGCGCCGACCCATGTACTCTGCGTTGATACCACTGCTTCC10.8130081300813009Clontech SMART CDS Primer II A (96% over 26bp)
CCTGGACAGCATGTGTCCGGCCTCCACACCCAGCGTACTCAGCTCTGAGC10.8130081300813009No Hit
GGCCTATAGTGAAAAAGAAAATATCTTCTGATAAGCAAGAAATTTTCTGA10.8130081300813009No Hit
CCCAGAGACAAATGTGAAGGAGACTCTGTCCTCCAAGCTGTCTCTTATAC10.8130081300813009No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCCTGGCGAG10.8130081300813009No Hit
GCTTGATGTACTGGTGATAGAGGTCGAGCTGACCCTTGGGCCTGTCTCTT10.8130081300813009No Hit
GGTAGGGGACCCATTTCCTCTCCTCAGCTCCCCAGAGCACAGAGTTGAGA10.8130081300813009No Hit
GTGTAGATCTCGTGGCGCCTACTCCCATGTACTCTGCGTTGATACCACTG10.8130081300813009Clontech SMART CDS Primer II A (95% over 23bp)
CATTGATAGAGCTTGCCAGTGTGCCCGGGTAATTTCTATCAACTCTTTTC10.8130081300813009No Hit
GGGAGCGCTGACCTCCAAGCTCACCTCCAACGGCTCCCTGCGGGCGCTGT10.8130081300813009No Hit
CCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGATCGGCACCACGAATCTACACTTTTCTACACGAC10.8130081300813009No Hit
ACGCAGAGTACATGGGATAACGAACAATCTACACTTCCCTACACCTGTCT10.8130081300813009No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.8130081300813009Clontech SMART CDS Primer II A (100% over 26bp)
ACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGACAGAGATC10.8130081300813009No Hit
GGCATGGGCGATTCGATTGCGGCCGCCGTTCGCGCCACTGCGCAAGCGCA10.8130081300813009No Hit
CAATACAGGACTCTTTCGAGGGCCTGTAATTGGAATGAGTCCACTTTAAA10.8130081300813009No Hit
CTGCAGACGGAGGGTGAAGCTGCATATGGATGATTTGTCCCTGGAACATT10.8130081300813009No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGATGAG10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
CTCTCCTAGCACCTCTAGAGAAAGTGTGGCTCTGCAGACACACCTTGATG10.8130081300813009No Hit
GATCACTAGGTCAGGAGATCGAGACCATCCTGGCTAACATCTGTCTCTTA10.8130081300813009No Hit
GTACATGGGGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATAC10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAAAAGAGGGAGGTGGAATTGTTTTTTTTTTTTTTTTTAACGTTAGATAA10.8130081300813009No Hit
GTATCAACGCAGAGTACATGGGATGTACGGCGACCCCGAATCTACACTCT10.8130081300813009No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGTCTC10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GCGCAACTCCACCGGCTCACCCACCAGGGCGTGGGGCACCGAGTAGTAGT10.8130081300813009No Hit
GCTGTAGATCGTCAGCGCAACAGCACTTCTCGCCGGATTCGTCTGGTGGG10.8130081300813009No Hit
ACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGA10.8130081300813009Illumina Single End PCR Primer 1 (96% over 28bp)
CCCGTGCTCTTCAGTGTCCAAAGTCCGGAAAGGGCCGAGGCAGCAGGGGG10.8130081300813009No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTAACAACGCAGAGTACATG10.8130081300813009Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
TTGTTAAAGGGAATGAAAATCAAACTTGAGAGTCAAATACTAGTCAGCTT10.8130081300813009No Hit
GATCTACACTCTTCCCTACACGACGCAGGGAGAGCGTCGTGTAGGGAAGA10.8130081300813009Illumina Single End PCR Primer 1 (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAACACAGCTT10.8130081300813009No Hit
CGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGT10.8130081300813009No Hit
GGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAA10.8130081300813009No Hit
TCACCGACCAGGGATTATTTACCTTTGGTCCTGGGTATCCAGGAAATCCT10.8130081300813009No Hit
TCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAGGTGGAGCCGAGT10.8130081300813009No Hit
GAATGATACGGCGACCACCGAGATCTACACTCTATCCCAAAAACCTCTTG10.8130081300813009Illumina Single End PCR Primer 1 (97% over 37bp)
CTCTAACACAACAACAAGCTAAACCTATCTCTTAAACACATCACCAAGCC10.8130081300813009No Hit
GCTCCAAGACGGCCCATAAAGCCAAGCTCCGATCCGAAGGGGAGGTGGGA10.8130081300813009No Hit
CCCAGCGTTGCCAGGCCGATGAGGAGCAGGACGTCGTTCGGGTTCGCTTC10.8130081300813009No Hit
GTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACT10.8130081300813009No Hit
GAGTGACGAGGTCAAAATCCCAACAGATATTTCATCCGGTAGTTGCTTGT10.8130081300813009No Hit
CTACAGAGGCAGGCAGGTCTCCTTGAGGTGCGGTGGGCTCCACCCAGTTC10.8130081300813009No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG10.8130081300813009No Hit
TTTCAGAACAGTTCTGTCAAAAGAAAGGTTCAACTCTGTTAGTGGAGAAC10.8130081300813009No Hit
GCAGTCCGTCGGTAGGGAAAGAGTGTAGATTCGGCGCCTACCCATGTACT10.8130081300813009No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GGAGAGCCAGGGTGCGGACCACCCAGCCAACTTGCGTCTCAGCTCAGGGA10.8130081300813009No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTT10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
CAATAAAAAACCATCATAAGATCTCAAAAAGAATACACTAGTTGGTCGCC10.8130081300813009No Hit
CCGAGATGAGATCGATGGCACCCCCCAGGCAATAGCCCCTGTCTCTTATA10.8130081300813009No Hit
CATTACTAATTGCACACTACCTGATGCGCTGGTTCTTCCTCACTGTGGAC10.8130081300813009No Hit
ATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAAGAGTAGACCGTCGCCTATCTCCCATGTACTCTGCGTTGATACCA10.8130081300813009No Hit
GCCAGCACGTGCACACGCACAACCTGGCCATGGCCGAGTTCTCGCGTGAA10.8130081300813009No Hit
CCGAAAAGACGCCGGTGGTGGTCGTCAAGTAATCCGTCGTCAGCAGCCTG10.8130081300813009No Hit
ACGCAGAGTACATGGGATGACCGCACCAATCTACACTTTTCCCTACACGA10.8130081300813009No Hit
TCGCCGTCACCGGCGTCAACGACTGGCTGGCGGTGCAGGCGAGCGACGGC10.8130081300813009No Hit
CGGCTGGGACGTTTTGCCGGACGACTGGGAAAAAGCTCACGCCGTCGTGC10.8130081300813009No Hit
TCAGGGTTCGATTCCCCGTCACCCGTGGTCACCATGGTAGGCCTGTCTCT10.8130081300813009No Hit
CTCTTGTCGAAGTTTCTTTCGTTCCTCAGCTTCTCCTTCATATTTCCTGA10.8130081300813009No Hit
GCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GGCGCGTGCCCCGCCGGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCC10.8130081300813009No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.8130081300813009Clontech Universal Primer Mix Long (95% over 21bp)
TCCCCTTTCAGTGCTGCCATCTTGCTCCTCCTTTGTAGTGAGCCCTTTTG10.8130081300813009No Hit
AATGGTGGAATCCATAAAAATTATTCAACAGGCTTTGGAAGGACTTCCCG10.8130081300813009No Hit
GTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGTTCC10.8130081300813009No Hit
GCCTGGAGCCTGCGCCAGCAGCATATCCTGCCGCCGCTGACCGCCGATTA10.8130081300813009No Hit
GGAAAGAGTGTAGACTCGGTGTCGCCGACCCATGTACTCTGCGTTGATAC10.8130081300813009No Hit
GCGCCTGTGTGAATGCGGTTGATCCGGAGCTGATTTCGGACTTGTCGGCG10.8130081300813009No Hit

[FAIL]Adapter Content

Adapter graph