FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGGAAGAACAGCTGGGCCACTTCAATTCCAGCTTCGTGGTGTCGGCGCAG21.6260162601626018No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC21.6260162601626018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTCACCTTTGTTGAGGTGAGCTGCACTTCATGGGGTTGGGAGAGGAAT21.6260162601626018No Hit
GGGGGAGCCCGAGTTCCGCTACACTGCTGGGATCCATGGCAACGAGGTGC10.8130081300813009No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTGACCGTCTGCGACCCCTTGGTGTCGACGATCTTCAGCCGGTCGAC10.8130081300813009No Hit
CTACACGACGCTCTTCCGATCTGTTCTGGTATCGACAAGACCTGGGACCT10.8130081300813009Illumina Single End PCR Primer 1 (100% over 22bp)
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC10.8130081300813009No Hit
CCTAGGTACGGGCAAGGAGCAACATATTACCATCACCGCTTCGAGCGGCT10.8130081300813009No Hit
GGCAAACATGTAGCAGGTTTACCACATGAGTGCCTTTTTTAAACTGTAAG10.8130081300813009No Hit
TTTCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.8130081300813009Illumina Single End PCR Primer 1 (100% over 22bp)
GAGTAAACACGACATCACTGTTCCAGAAACACAGAGAAAGGACTGTCTCT10.8130081300813009No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGCAAGCGTCGTGTAGGGAAAGAGTGTAATCTCGGTGGTCGCCGTATC10.8130081300813009Illumina Single End PCR Primer 1 (96% over 25bp)
GATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGC10.8130081300813009No Hit
CCCTCCTCCAGTCCTGGAACAGGGAGTCTGAGCTCTCGAAGCTGTCCGCA10.8130081300813009No Hit
GTGATTGGCTTAGTGGGGGCAAAATTATGCTTTGTTGTTTTGTTGTTATG10.8130081300813009No Hit
GAACAAGTCGCTGCTTCCCCGGGTGAGGAGCTTTTGCGCCGAGCATCGCG10.8130081300813009No Hit
ATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTG10.8130081300813009No Hit
TTCCTACACGACGCTCTTCCGATCTGGGCCTCCAGTTCCTGAATGAGGAA10.8130081300813009Illumina Single End PCR Primer 1 (96% over 25bp)
GCATGTAACAATCTGTTGTTTCAAAGGTCAAATCGATTGTTTTTATGTCA10.8130081300813009No Hit
ATGTTAGCCAGGATGGTCTCGATCTCCTGACCTAGTGATCCTGTCTCTTA10.8130081300813009No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCGGCGACACC10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTT10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GTATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTG10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
CTAGATGGCTTTCATTCTTTCCGATCAAAAGAGCAAGTAAAAGAAAATGC10.8130081300813009No Hit
ACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCGCCGC10.8130081300813009No Hit
GCCTGACTGTAGGAGTAGCTGCTAACATCCTAAAGTTAGTGGCTGGGAGA10.8130081300813009No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTAGACCGTCGTGA10.8130081300813009No Hit
ATCGACCCTCGACAGGCTGTCCAAGGCCAGATACCTGCTTGTGCTGTCAA10.8130081300813009No Hit
GGCCACAGGCCTGGAAGGTGTGTGGACCTGCACGTGACTGTTTGGTGCAT10.8130081300813009No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCAGCAGACATTTCTTTCTTTCAAGGTCAATTTATTTTAGATGAATTTA10.8130081300813009No Hit
CTACACGACGCTCTTCCGATCTATCCCTTTTTTTGGGATAGATCGGAAGA10.8130081300813009Illumina Single End PCR Primer 1 (100% over 22bp)
TCTGTATCTGGCTGCTGCCTGCGTGCCGGGCCGCTCCCCCATCGGTGGCT10.8130081300813009No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGGTCTTTTTT10.8130081300813009Clontech Universal Primer Mix Long (95% over 22bp)
CCCATGTACTCTGCGTTGATACCACTGCCCCATGTACCTGTCTCTTATAC10.8130081300813009Clontech SMART CDS Primer II A (95% over 24bp)
ATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGATACGCGACCACGA10.8130081300813009Clontech Universal Primer Mix Long (96% over 27bp)
TGGCAAAAATCAGACCCTGTTCTGGCAATTAAGCAATGGGTTCATGAAGG10.8130081300813009No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGAAGTGTAGATTGTTCGTTATCCCATGTACTCTGCGTCTGTCT10.8130081300813009No Hit
CTGTCCCAAGTTCTGCAGGTACAGAGATCGGAAGAGCGTCGTGTAGGGAA10.8130081300813009Illumina Single End PCR Primer 1 (100% over 26bp)
AAGCAGCGGATTTCTAAATTTTCATATGGCCCGCCGGGAAGTCCTTCCAA10.8130081300813009No Hit
GAATTGGCAAGGTGTACCAGATAGCAATATCTTTGCCTTCTGACTTCCAT10.8130081300813009No Hit
AAACTAGACAGAAGCATTCTGAGAATCACGTTTGTGATGTGGGTACTCAA10.8130081300813009No Hit
CATATAAACCTTGTTCAGAGATACGCTCTGCTGCTGCCGTTTTCTGGGTT10.8130081300813009No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCA10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCTGTT10.8130081300813009No Hit
TCTCCACATCCCCCCCTGCCAGGTCACATCCTTCGGCATGGCCCGCACCT10.8130081300813009No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8130081300813009Clontech SMART CDS Primer II A (100% over 21bp)
TTTTCGAACAAGCCCTGCGACGCGAGCTGTCCTTTACCGCCGGCGCCGTC10.8130081300813009No Hit
CATCACAAAGCAGTTTCTCAGAAAATTTCTTGCTTATCAGAAGATATTTT10.8130081300813009No Hit
GAATCAGCTGAAGGAATTGCCAGAAAAAATGCCCAAAACTCTTCAGGAGC10.8130081300813009No Hit
GCTCCCATGTACTCTGCGTTGTTACCACTGCTTCCCATGTACTCTGCGTT10.8130081300813009Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCAT10.8130081300813009No Hit
ATGCCGCAGCCTTCGACGAAGCCGCGCGGCGGCGCCGAGCCGATGCGGGC10.8130081300813009No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG10.8130081300813009No Hit
AGGAAAGAGTTCGAAGAGGAGAGTCCTGTCAAATATAGCCAAATGGTCAA10.8130081300813009No Hit
CTTCAGGCCGTGGCGGCCGCCCTCGCCCGGGTATTGCGGGTGGACAGCAA10.8130081300813009No Hit
TCACATAGGCTGGAAAGATTTCACCGCCATCTGTAGGCGGTGAAATCTTT10.8130081300813009No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC10.8130081300813009No Hit
GTGATTGGCTTAGTGGGGGAAAAATTATTCTTTTTTGTTTGGTTTTTTTT10.8130081300813009No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.8130081300813009Clontech Universal Primer Mix Long (100% over 23bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAATGGGTTTAA10.8130081300813009No Hit
TGTCTGCTGTGATCCTCACAGCTGTTGCTGTTATTACAGTCCAGCTTAGA10.8130081300813009No Hit
CCCTACAACCTAAAAACAAGAAAAGAAACTTAGAATGTGGTTTATCGACG10.8130081300813009No Hit
GTACATGGGAAGCAGTGGTATCAACGTAGAGTACATGGGAAGCAGTGGTA10.8130081300813009Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
TCCGATCTCACTGTCTACTGGTACCAGCAGGGAAGAGCGTCGTGTAGGGA10.8130081300813009Illumina Single End PCR Primer 1 (95% over 21bp)
GGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCG10.8130081300813009No Hit
GCCCTATGCCACGGAGCCTCGCTCATTGCTAGCACAGCACTCTGAGATCA10.8130081300813009No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTAGTTTCCTGTGGTTGTTACAGCAAATTCACCACCAACTTGGTGCT10.8130081300813009No Hit
ACGCCGATGTAGTTGCGCGTGGCCACGCGGCCATCGGGGCGCACGATGCC10.8130081300813009No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACCATTGGAATAAAAACAA10.8130081300813009No Hit
GAATGAAGTAAACATACCCTCTTCTTTCTCAGCAGGCTTCTCCTCTTCAG10.8130081300813009No Hit
ACTGCCGGCGTTGGCGTTGAGCAGGATCTCGCCTTCGAGCAGGCGCACCA10.8130081300813009No Hit
CTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTGCGC10.8130081300813009No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGGCGA10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGATTCATACTGTTTATCCAATTTCTTATCCACACAATGGACAAGAATG10.8130081300813009No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC10.8130081300813009Clontech Universal Primer Mix Long (96% over 25bp)
GGGGTGATGCGAGGGGGTGGTGCCCGAGACCGGAATCGAACCGGTACGGC10.8130081300813009No Hit
CCTCAGCGGGTGGGAACCTCGGGGCCCAGGGGTGGAGCTGGCGTCCGCGG10.8130081300813009No Hit
CTTGGAGGACAGAGTCTCCTTCACATTTGTCTCTGGGCTGTCTCTTATAC10.8130081300813009No Hit
ATGCACGCTCAGCGTGATGCTGACACACCAGCCCCCTGCTGCCTCGGCCC10.8130081300813009No Hit
GTACATTGGAATGATACGGCGCCACCGAGATCTACACTCTTCCCTACACG10.8130081300813009Illumina Single End PCR Primer 1 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGATACGGCGA10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTACCATGGTGACCACGGGTGACGGGGAATCGAACCCTGACTGTCTCT10.8130081300813009No Hit
GATCAACTAATAGTTCGAGCCCAGGGCCTTTACTTCTTGCGTTGGGCTCG10.8130081300813009No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTC10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
GACAGGAAGAGCGTCGTGTAGGGAAAAGTGTAGATTGGTGCGGTCATCCC10.8130081300813009Illumina Single End PCR Primer 1 (95% over 22bp)
CAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTACACTCTT10.8130081300813009Illumina Single End PCR Primer 1 (95% over 22bp)
GGTACACAGAAGTCAAGAATTGAGGTTTGGGAACCTCTGCCTAGATTTCA10.8130081300813009No Hit
TTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCC10.8130081300813009No Hit
TATCAACGCAGAGTACATGGGTGACGGCGACACCGAGATCTGTCTCTTAT10.8130081300813009No Hit
GTGATTGGCTTAGTGGGCGAAATATTATTCTTTTTTTTTTGGTTATTTTG10.8130081300813009No Hit
GTTCGTACATCGGCACCGATCCAATCTCGCTGATCCAGACGTTCGGCAAC10.8130081300813009No Hit
TTGTAGGCCGGATAAGGCGTTCTCGCCGCATCCGGCATAAACAAAGCGCA10.8130081300813009No Hit
CATGGAATCGCGCACCCGGCAAACACATTCCCCAAACGACGCACTTACGA10.8130081300813009No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTTCCTTCTGCCCGCCCTCAAAAGGCAAAGAACCACTGCGGGCTGATGG10.8130081300813009No Hit
ATGTTCAGGCGCCGCGATTCCAGCACGAGCCGCTCTGCCCGCTCGGGCGG10.8130081300813009No Hit
AGATTGATTAACGCACGACGGCGTGAGCTTTTTCCCAGTCGTCCGGCAAA10.8130081300813009No Hit
GAACAGAATTAAGAAGAAAGAAGCCTACTTTACATATCCAATATGGATGA10.8130081300813009No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAC10.8130081300813009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCCGAACAGCGTGCTATAGGCCTCCAGCTGTTCCGCCGAGGCACCCGG10.8130081300813009No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGCGACCAC10.8130081300813009Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGAGCTCGATGGCCACTACTACTCGGTGCCCCACGCCCTGGTGGGTG10.8130081300813009No Hit
GGCGAAGAATGTTACTGCCAAGATGGCATTGGAGGTGCTGAACACTCGAC10.8130081300813009No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.8130081300813009Clontech Universal Primer Mix Long (95% over 21bp)
GAGTAACTTTTAAGTGGAAACCAAGTTTAGATTTGGGGAGTGGTAAAGGA10.8130081300813009No Hit
GGGCTATTGCCTGGGGGGTGCCATCGATCTCATCTCGGCTGTCTCTTATA10.8130081300813009No Hit
GCCCAAGGGTCAGCTCGACCTCTATCACCAGTACATCAAGCCTGTCTCTT10.8130081300813009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)