FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTACCGCCTCGCCTATGCGCAAGGCCAGCGTACCGACGCCTACGCCA42.515723270440252No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42.515723270440252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCAACTGCATCACCGAATCCGTCAGCCCATCCGGGTCGAACGGCTTGG31.8867924528301887No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.257861635220126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGGAAGGCCTGGGCAAGGAGCCTTACGAAGCGCACCGCATCGCGCAGAC21.257861635220126No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.257861635220126Clontech Universal Primer Mix Long (96% over 26bp)
TCTCGGCGCAGCTCGAATCGCACTTCGGCGACAAGGTGTTCAAGACGGTG21.257861635220126No Hit
GCCATATAATGCGCCAGTTTGTCGCAGCAGTCCCCCAAAGGCCTATCCCG21.257861635220126No Hit
GCGTGAAGTGGCAGCCCGCCTGCAGGCCTGCGCGCGTGAAGCCGACACCG21.257861635220126No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC21.257861635220126Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTGCGTGACAACGCGTGCATCATCAATGTGAGGCCGGGCAGGATCAG21.257861635220126No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.257861635220126Clontech Universal Primer Mix Long (96% over 26bp)
AGCGTGCGACTAGAGGCTGAGTCAGTTGAGGCCTCTGGCCGTGTCCCTGT21.257861635220126No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.257861635220126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGCTGCTGCGCAATGGCCTGAAGGTGACGCTGGTGGTGACGGCCAGCAT21.257861635220126No Hit
GTTCCAAGAACCCAGAGGAGGCAGAACTAGAAGACACACTCAATCAAGTG10.628930817610063No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
TTCTTTGGTTTTCCAACGGTCTTTACTGTTACCTCACCACCTTCCAATAG10.628930817610063No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT10.628930817610063No Hit
GCCACATATTGCAGCAGCGCAGCGCCTTCGGTGTGGCGCGAGCCGTCGGC10.628930817610063No Hit
CATCCTGGTGCTGACCGCGCCGGGCAATGCGCTGGAGTCGCCGCGCGTAC10.628930817610063No Hit
CTACAAGTTCGTGGCCATCGACGACGGCCCCGCGCTGCGCGAGGACCTGC10.628930817610063No Hit
GACTAATACAGTATCTAATCTTCAAATGAAGAAACCAACCAAAGGGGTAT10.628930817610063No Hit
CTTCGGGGCTTGCCGGCGGCAGTACAATCGCGCGTCCGCGTGGTCGGCTC10.628930817610063No Hit
ATGCGGACTACCCGCAGCAGTTTGCCGATTTCAATGCGATCGCGTCGATC10.628930817610063No Hit
GGATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACCTCA10.628930817610063Illumina Single End PCR Primer 1 (97% over 45bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
TGGGTAGGGACCACCGAGATACCTCTTTCCCTACACGACGCTCTTCCGAT10.628930817610063Illumina Single End PCR Primer 1 (96% over 29bp)
GCCGGAACAGCAGACGCGCCGGACGCATCAGCAGCCGGCGCGCCGCCTCT10.628930817610063No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCA10.628930817610063Illumina Single End PCR Primer 1 (100% over 33bp)
ACCCCGAGATCTACATCTTTCCCTACACGACGCTCGTTTTTCAGGACCTG10.628930817610063Illumina Single End Sequencing Primer (95% over 22bp)
GCATCCAGGAACACGAGTTGACGCAGCCGGTCGCGGGCGCGGTCCGCCAC10.628930817610063No Hit
GCCTGTTACCAGCTACTTGGGAAGCTGAGGCAGGAGGATTGTTTGAAGCC10.628930817610063No Hit
CGATATACGCGGCCGGTGGGGTCGTGCAGGCCAGTCTGGCCTTGGTCGGT10.628930817610063No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCTGCGTT10.628930817610063No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.628930817610063No Hit
GTATTAACGCAGAGTACATGGGAATACGGCGACCACCGAGATCTACACAC10.628930817610063Illumina Single End PCR Primer 1 (96% over 27bp)
CTTGAGGAGTTAGCCACAGAAACTGGACATCCAGACAGGAGAGGCCGACG10.628930817610063No Hit
GCCCCCCGCAGGCGCCGCGGCACCGCTGTCCACTCCCGCAATGCCGCCCG10.628930817610063No Hit
GAGTACTTTTTTTTTTTTTTTGCGTTGATACCAATGCTTCCCATGTCTGT10.628930817610063No Hit
CGGTGCTGCCACTGAGATTGTTCGCGTGTGGTCGCCAGTCAGCAGGGATC10.628930817610063No Hit
GCGCCGTCTTCGCTCGACACCGGCAGGCGTTGCGGCTCCAGCGGAAACAC10.628930817610063No Hit
TTCCGGGCGCGCATTGATGACTGCCTGATACCACAGTTCCGCCTGCGAGC10.628930817610063No Hit
TAGCCAACGCGTCGCCAAACACTGTCACACTGGCTGCCACACTGTGAGGC10.628930817610063No Hit
TGCGCCGCCAGATCCGCACCGAAGAGATCCGTATTCTCGAACCCGCACCC10.628930817610063No Hit
CGTTCAAGCGTCGCCTGATCGATAACATCTGCCGTAAAAACAATATCATC10.628930817610063No Hit
TATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATG10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAGATATTACCACACAGAAGAGGAGAAAGCACAGTGACCACAGAGGCA10.628930817610063No Hit
ATCGAGAATCGAAGATCAAGACCGAAAAGTTGATCCGCGAGGAACGCGGC10.628930817610063No Hit
ACGCAGAGTACATGGGGGCAGACTACACTTTCCCTACACGACGTCTTCCG10.628930817610063No Hit
TGCCTGCCGATGACTTGACCGACCCGTCGCCGGCAACGACCTTCCTGCAC10.628930817610063No Hit
AATCCAATCACACTGTACTCAGTGATCAAATAGCCCAGGCACCATGCCTC10.628930817610063No Hit
GTTGTGAGGGCCTCATTGTCAGGGTTTATGAATTTCTCGTTTCCTTCTTT10.628930817610063No Hit
CCATTTTTGTTTGCTTCTTGGATGAGTTCAACTGCTAGTGCGCGATCTGA10.628930817610063No Hit
CAGCCGTATCGCCTCGAAATGGGCACGCGCCTGGCCAATCCGCGCGGCAA10.628930817610063No Hit
CACCATCGACGACCTGCGCAGCCACCTGGCCGCCTTCAGGCGCCCCGCCT10.628930817610063No Hit
AAACGGCGTGCCTGAACAACACGCGCGCCGCCATGACCACGCTGCCGGCA10.628930817610063No Hit
GGGGCGCAGGTACACGCTGACCGTCGCCGAGGGCGCGGAGGCGGGTCGAC10.628930817610063No Hit
GCGTTCAAACTCCCGCAGCACGGCTTCTTCCACGAGGTCCGTCAGTTCAT10.628930817610063No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATGTGCGTTTATACC10.628930817610063No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAA10.628930817610063Clontech Universal Primer Mix Long (95% over 23bp)
ACCGAGATCTACTCTTTCCCTACACGACGCTCTTCCGATCCTGTCTCTTA10.628930817610063Illumina Single End PCR Primer 1 (96% over 31bp)
ACCAGGGCCGGATGATCATCAGCACGGAAATGCGCTACGGACACACGTGG10.628930817610063No Hit
ACGCAGAGTACATGGGAATCGGCCACCGATCACTCTTCCCTACACGTGCT10.628930817610063No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGGGGCGCTCGCGTGCGGCAAGCGCGAGCTGCGCGAGGAAACCGGTT10.628930817610063No Hit
GTACATGGGATACACCGAGATCTACACTCTTTCCCAGCAACTCCAGTCCT10.628930817610063Illumina Single End PCR Primer 1 (95% over 23bp)
ACAGAAGCAAGTCCCCGCCGAAGCGCCCGCCCCGTCTGCGCGCCCGTCTT10.628930817610063No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GCTGATGAATGCGAGGAACATCGCCTTTTCACGCTCACTGCGAACGGCGG10.628930817610063No Hit
AAGTGAGATACGCGGCCAGCGGCCCCGCCAGGCTCTTCATCAGCGCGCGG10.628930817610063No Hit
GATATAGACTATCAAGGAGTCCTGATTATTTCACTTTTTAAACCAGAAGA10.628930817610063No Hit
TGCCCGTGTAGCCTAACTGGGAGACACCTCTCAGTAGGGGCCGACAGACA10.628930817610063No Hit
GAGTACATGGGTGAACGGCGACCACGAGTCACACTCTTTCCCTGCTGGTA10.628930817610063No Hit
GCTCAAACAGGGCTCCTTCCTTTTTCCTCCCCGCGACAAAAATCCAAGGC10.628930817610063No Hit
GTTGATACCACGGCTTCCCATGTACTCTGCGTTGCTGTCTCTTATACACA10.628930817610063No Hit
GGGAATGTTCAGGCCGACGATGTCCGCGTCGGAGATGCCGTCCAGGTACT10.628930817610063No Hit
GTCTGCTCTGAGAAAAGAGCCAAGTTCAGGGCAGAGACTACCTTCCTGGG10.628930817610063No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATA10.628930817610063No Hit
CAACGGCGCGATTCCGGGCGCGGCGATGGGCTCGCCCGAGCAGTTCGAGC10.628930817610063No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.628930817610063Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGTGTACGGCGACCACCGAGATCTACACTCTT10.628930817610063Illumina Single End PCR Primer 1 (96% over 29bp)
TTTGTGTTTGTGGTTCATCCCTGGTTTCATCCCCTGCCGGGGCTGACTCA10.628930817610063No Hit
GTGGACAGCCCGCGCAAGCTCTACACGATGGGCTTGCTGACCAGCCTGCT10.628930817610063No Hit
AGTGTAGACTCGGTGTCCCGTCCCATGTACTCTGCGTTGATACCACTGCT10.628930817610063Clontech SMART CDS Primer II A (96% over 25bp)
CCCCAGCACCGTCACGCGTGCGCCCTCGGCTGCCGCCGCGCGTGCCACGC10.628930817610063No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA10.628930817610063No Hit
CTCTTTCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGATC10.628930817610063No Hit
CTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTA10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATCGGCGACACCGAGATCTACACTCTTCCCTACACGACGCT10.628930817610063No Hit
CTCGCATCCTTTCGGGCAGTCGACGGACTCGCTCCCGGAGTGCAGGGAGC10.628930817610063No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAA10.628930817610063Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.628930817610063Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC10.628930817610063No Hit
TGGGACAGGAAGAGCGCGTGTAGGGAAGAGTGACGTGGTCGATCATTCCC10.628930817610063No Hit
GAATGCCCGGATCAATCGACGACAGCCCAAGCAGTTGCGCGCCGCCACCG10.628930817610063No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGCTGTCTCTTATACACATCTGA10.628930817610063Illumina Single End PCR Primer 1 (96% over 29bp)
CAAACCGCCCAGGGCCAGATGCGGCACGAGCGTGATCGGTGCCACACCGG10.628930817610063No Hit
GTACATGGGACCCCACTCCCTCAGCAACTCCAGTCCTTGCATCATGAGAT10.628930817610063No Hit
ACACGAAGTCACGCGGCGCCAGATCCTTCAGCGTCGGGGCATAGCGCTCC10.628930817610063No Hit
GGTTGGTGCCTGCCGCCACCGGTGTGCCCGATGCGGTGAGCGCCCCGGTG10.628930817610063No Hit
ATTCTTGCCACCGACCCCGGCGCCACGCGCGACTTCCAAGCGTTCGCCAA10.628930817610063No Hit
GGGCTGGCGAGTGCCGGCTCCGCGATCCTCCGGACAAACGATGTCGAATC10.628930817610063No Hit
GTATTCGGATGAGCGCCACCTCTCGCTGGTGGCCGCTGCGCAGACGCAGC10.628930817610063No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATCT10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA10.628930817610063No Hit
TCGCTGCCGTGCGGGCAAGGGTGCCCCACCGTTTGCCGTTTTTGACACAA10.628930817610063No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGACTGTCTCTT10.628930817610063Clontech Universal Primer Mix Long (96% over 25bp)
CGCAAGCATAGAGCAATCGTCAAGAGTGGTCACGCAAGCGGACGGCCGAT10.628930817610063No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTTAAACAACATGATC10.628930817610063No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCAGCGTTGA10.628930817610063Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGTAGGCCACGAGATCTCACTCTTTCCCTACACGACGC10.628930817610063Illumina Single End PCR Primer 1 (95% over 22bp)
CAACGCAGAGTACATGGGAAGCAGTGGTACTGTCTCTTATACACATCTGA10.628930817610063No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGGTATTCCCTACACGACGC10.628930817610063Clontech Universal Primer Mix Long (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCCTGTCTCTTATAC10.628930817610063Illumina Single End PCR Primer 1 (97% over 37bp)
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCCTGT10.628930817610063No Hit
GTGAGGAATAAATATTTGTTATTTATAGGCCACTCAGATTATGTTGTTTT10.628930817610063No Hit
GCGTACATCGGGCCCTTCCACTCGGTCAGCTTTTCAGCCGTCAGCCCTTT10.628930817610063No Hit
CACTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGGCCTG10.628930817610063Illumina Single End PCR Primer 1 (100% over 32bp)
GTACTCATGCACGGACTGCGCCCAGGTCGGGTCGGCCATCGTGGGCCAGC10.628930817610063No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACCTGTCT10.628930817610063No Hit
TTATATGGCCGCGATTGACCCCGAACAAGGTAAAGCGCCTTTATGCAAGT10.628930817610063No Hit
GTGTAGGAAGAGTGTAGATCTCGGTGTCGCTCCCATGTACTCTGCGTTGA10.628930817610063No Hit
CACGCTGTCGCCCTCACGGTAGAGCGCATCCATGAGCGTTTCGCGCGGCA10.628930817610063No Hit
GTAAAGTGTGTTTGCACATATTGAATGAAGATCTGCACATCAGGGGAGGG10.628930817610063No Hit
GCTGCTGGCTGCGCACCTGCTGGAAGTGGTGCTGTGCTTTCGCAAAGTGG10.628930817610063No Hit
CCTGCGGGCGGCTGGTGCGGCGAGGGCAGCGTCATCAAGCGCGAAGACCG10.628930817610063No Hit
GAACATCGACGCACTGTAGGCCTGAGCTGTCAAATCCGGCTTGCCGGAGC10.628930817610063No Hit
GTCGTGGGTCGATGGCGTATGCCTTGTGTTAACGGTTGGCCGGGGTTGGC10.628930817610063No Hit
GGCAATTAGGGGCTTATTTTGGCAGCCTGGAGCTGGGTGGAGAGTCTGAG10.628930817610063No Hit
GTTGTCGACGCCTATGCGTGTGCTGCATGACAATGGCGCTTCGCCAGAAC10.628930817610063No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.628930817610063Clontech Universal Primer Mix Long (95% over 21bp)
GCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACAGCA10.628930817610063No Hit
CTTCCATCTTCTGGGCCTGGCGCAATTGCGCTTCCACTTCGGCGCGGGCA10.628930817610063No Hit
CTACAAGCTGTACCAGGAACATATTCACGTCCACGAACAGGAAGAGGTGC10.628930817610063No Hit
GAGTACATGGGAATGTAGGCGCCACCGAGACTACCTCTTTCCCTACACGA10.628930817610063No Hit
GAACTTGGGACAGAGCGTCGTGTAGGAAAGATAGCTGTCTCTTATACACA10.628930817610063No Hit
GTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.628930817610063Clontech Universal Primer Mix Long (96% over 27bp)

[FAIL]Adapter Content

Adapter graph