FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003680590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA125.0Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACTATGTACTGCCATGAATAGATCTGTCCTGGATCAGTGCTATCCTTC125.0No Hit
CACTGTAAAAAAAACACATAACAAGCTACCCACTAGAATCAAATCACAGT125.0No Hit
GTACATGGGATGATCGGCGACACCGAGATCTACACTCTTTCCCTACACGA125.0Illumina Single End PCR Primer 1 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)