FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG41.5037593984962405Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41.5037593984962405Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTGTCTGTGAAAAAGACCTCAGCCCTGGGGCCAGCGGAGGGTATACC41.5037593984962405No Hit
AGCCCAAGAGCGGCGCGCCGGTGCTGCGCGTGCATGGCACGACCGGCCTC31.1278195488721803No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG31.1278195488721803Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG31.1278195488721803Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT31.1278195488721803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT31.1278195488721803Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31.1278195488721803Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.7518796992481203Clontech Universal Primer Mix Long (100% over 23bp)
ATGAAATTCTGGGTTGAAAATTCTTTTCTTTAAGAATGTTGAATATCGAC20.7518796992481203No Hit
CTCTTTCCCTACACGACCCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGT20.7518796992481203Illumina Single End PCR Primer 1 (96% over 30bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.7518796992481203Clontech Universal Primer Mix Long (96% over 26bp)
TCCAACGGTTCCGGCAGGTCGACGGTCAAGGCGGACATGGGATCAGTCTA20.7518796992481203No Hit
AGATAAAACTAAGACAAGGATGAGGTAAATAAATTGTGGGACCACTGGGA20.7518796992481203No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.7518796992481203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.7518796992481203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTGGCGCTGCCGTCCTGCGAGTTGTCGCGCGCAAGGCCCGCGAAGT20.7518796992481203No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACATC20.7518796992481203Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA20.7518796992481203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTAGGGAAAGAGTGTAGATCTCGGTGTGCGCACCCATGTACTCTGCGT20.7518796992481203Illumina Single End PCR Primer 1 (96% over 28bp)
CTAACATCACAATTAAAAGAACTAGAAAAGCAAGAGCAAACACATTCAAA20.7518796992481203No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20.7518796992481203Clontech Universal Primer Mix Long (96% over 26bp)
GAAATACGTGCTGGCCGACAAGCTGGCAGCGGAAGTCTTCGAGAAGGCCA20.7518796992481203No Hit
GTACATGGGTGTACGGGACCACGTTACACTCTTTCCCTACACAGCAACTC20.7518796992481203No Hit
GATAACAACAGATACTCAAAAACCAAAACTAAAAAATATACAAAAAACAC20.7518796992481203No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACACAACACGGGAAACCTCA20.7518796992481203No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT20.7518796992481203Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.7518796992481203Clontech Universal Primer Mix Long (96% over 26bp)
CTGCAGTCGCGCCTGTTCTCGTATGCCGACGCCGCGCGCTACCGCCTGGG20.7518796992481203No Hit
GGTCGAGTATAGACCGATTCGATGCGTGGAGGGGCGGCAGTTCCACCGGC10.37593984962406013No Hit
ATTTCCCCCACTTTATGGAGGAAGAAACAGTAGCCAATGAGTGACAAGAA10.37593984962406013No Hit
GCAGTGGTATCAACGCAGAGTACATGGGTGATACGACCACGAGATCTACA10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
CCCACGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
GAACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTTGATCTGTCTC10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTTAA10.37593984962406013No Hit
CACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGACAGAGATCG10.37593984962406013No Hit
ACACTCTTTCCCTACACGACGTCTTGCCTCAGATTCTGTCGGTACCAGAG10.37593984962406013Illumina Single End PCR Primer 1 (95% over 21bp)
CCCTTTAAGACACTATTTATCTTCCATTATCATCAATTCCAAGGTCTGGT10.37593984962406013No Hit
GAGGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
TCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCT10.37593984962406013No Hit
GTAACTGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGGCGAC10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTACAAGACTCCGGTGGAAATCGCCGCCCTGCGCGCCGTGCGCGATGCGC10.37593984962406013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCGATCTGTACTGGTATCGACAAGACCCGGGAGAGCGTCGTGTAGGG10.37593984962406013No Hit
NCCTAGCACTTTGGGAGGCTGAGGTGGGAGGATTGCTTGAGTCCAGGAGT10.37593984962406013No Hit
ACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.37593984962406013Clontech Universal Primer Mix Long (95% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTTGTAGCGCCGA10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTAT10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTACATGGGTATACGGCGACCACCGAGATCTACACTCATTACCAGACACG10.37593984962406013Illumina Single End PCR Primer 1 (96% over 30bp)
ACCAGGACTCACGCCAATTTTGTGACTTCCGTTCTCTCTACTGTCTGTCT10.37593984962406013No Hit
TCCCTACACGACGCTCTTCCGATCTCTGGTACCTGTCCCAAGTTCTGCAG10.37593984962406013Illumina Single End PCR Primer 1 (100% over 25bp)
CCTCGATATGAGATCTTCGTTTCGGACAAGGGCCTAAACCAGGACTGTCT10.37593984962406013No Hit
GTTGATGGGCATTTGGGTTGATTCCAAGTCCTGTCTCTTATACACATCTC10.37593984962406013No Hit
CCGTATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGATCGCGCCACCGAGATCTACACCTTCCCTACACGACGC10.37593984962406013No Hit
CTCATGCTTTCACTGTTGAAAGCTGGACTGGTCTTAAATTTTTACGATCA10.37593984962406013No Hit
CCCCTCCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGC10.37593984962406013No Hit
CTGCTAGACAGAAGAATTCTCAGTAACTTCCTTGTGTTGTGTGTATTCAA10.37593984962406013No Hit
CAATAACACCCAGGACTATACAGCCTGCCTGCAGGATAGGGCACACAGCA10.37593984962406013No Hit
GGTCAAGAAGGGTGAGCCGTACCTCGTCAACGACAAGAAGGAGCGCAAGG10.37593984962406013No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT10.37593984962406013No Hit
GGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATG10.37593984962406013Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGCTGCCTTATTAGCCCTAGCTGATTTTTTCTGCCCTTCAAAGACATTT10.37593984962406013No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTGGTCCGTATCTCGTATG10.37593984962406013No Hit
CCCGCCTAGAGGCAGGTGGATGTGCTGTGCGGGCTTCCCCTGGGCGTTTG10.37593984962406013No Hit
GCCTGTTTTCTGGGCTACTTAGATTTGTGACTTTTGGCTGGGCATGGTGG10.37593984962406013No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGGTGATCTCGTGATCTCCCATGTA10.37593984962406013Illumina Single End PCR Primer 1 (96% over 27bp)
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGAA10.37593984962406013Illumina Single End PCR Primer 1 (100% over 35bp)
TCATTGCACACTGTGGCCTCGAACTCCTGGCCATCAGCAATCCTCCTGCC10.37593984962406013No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
CCCTACACGACGCTCTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGC10.37593984962406013No Hit
CTCGCGCGCTCCGCCGGGGTGGACCCGAGCGATCCGGCGACCTTCCTCTT10.37593984962406013No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTATACACA10.37593984962406013Clontech Universal Primer Mix Long (95% over 21bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTG10.37593984962406013Clontech Universal Primer Mix Long (96% over 28bp)
GCGTACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
GCGCGACAGGTTCTCGACCTTCGCATGCTTCTCGAAGCCGCGAAGTCTGT10.37593984962406013No Hit
GAACTTGGGACAGGTGTAATCTCGGTGCCCGTACCCATGTACTCTGCGTT10.37593984962406013No Hit
CAGTTGTATCAACGCAGAGTACATGGGTGATACGGCGACACCGAGACTCA10.37593984962406013Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
CCGCCGCCACCAGCCGATCAGCTACCGCTCAGCGATCTGACGAGAAAATT10.37593984962406013No Hit
GTACATGGGTACGGGACCACCGAGATCTACACTCTTTCCCTACACCTGTC10.37593984962406013Illumina Single End PCR Primer 1 (96% over 33bp)
ATCTTCGCCCAGTTGCTTGACGCCCAGCGAAACGCGGTTCTTCTCTTGGT10.37593984962406013No Hit
GAGTACTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGT10.37593984962406013Clontech Universal Primer Mix Long (95% over 21bp)
GGTAGTAGTTTGCCTCGGCCTCAGCCGGTGGGATGTAGCCCAGTGGCCCC10.37593984962406013No Hit
CTACAACCTTCACAACAACCCAAAAAATAAAAAATTACAAAAAAAAACAT10.37593984962406013No Hit
TTTCTCCAGATTCCTTAACTCACAGAAATTTTTCTTTTAAGAGGTTTCAG10.37593984962406013No Hit
GGGAAGCAGTGGCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.37593984962406013Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAATGTACGGCGCCACCGAGATC10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAGATC10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
CACCAGCGGCGGATCGAGCGAGACCGAGGTGAAGCTGTTGACCGTGATCC10.37593984962406013No Hit
TGGGTTGATACGCCCCCGAGATCTACACTCTTCCCTACACGACGCTCGTT10.37593984962406013No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTATACA10.37593984962406013Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
GGGTGGGGTACAATGGCGCCATCTTTGCTCACTGCAACTTCTGCCTCACG10.37593984962406013No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.37593984962406013Clontech Universal Primer Mix Long (95% over 22bp)
AGTATACTTGAGGAGGGTGACGGGCGGTGTGTACTGGAAAGTGCACTTGG10.37593984962406013No Hit
CCTCGAGGGTGACGGAGGTGATCGGGCTGCCGGGCTCGAGCGCGACCGCC10.37593984962406013No Hit
GTACATGGGATGTACGGCGACCACCGAGATCACACTCTTTCCCTACACGA10.37593984962406013No Hit
GGTCTGGGACAGCGTCCACGAGCCCTTCGGCCTCTCGGCCCTGTTCTGTC10.37593984962406013No Hit
GGACAGCGTCGTGTAGAAAGAGTGTAGACTCGGGGTTCCCATGTACCTGT10.37593984962406013No Hit
GAGTGGCAGTCGGTGATCCTAGCACGTCTGCTGATTGACTGCAAACAGAA10.37593984962406013No Hit
GGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGAGGT10.37593984962406013No Hit
GGCCAATTCAAAAGCCCAGCCCGGGAGTCCGTCCACCACTGTCTCTTATA10.37593984962406013No Hit
CTATAAACTCAAAAAAAATGACAACAATATCTAAAAAACAACACAAAAAC10.37593984962406013No Hit
TCCCAAGTATTATGTATGGCAGCACAAAATAGACTGAGACACACCCTATG10.37593984962406013No Hit
GTATCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
CCCATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
TCGTGTAGGGAAGGTGTAATCCGTGGACTCCCATGTACTCTGCGTTGATA10.37593984962406013No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGCCCAAGTTCTGCAG10.37593984962406013Illumina Single End PCR Primer 1 (100% over 33bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGTGCGACCCGAGATC10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTC10.37593984962406013No Hit
GCCTTGGCCAGGTTGGGATGCAGAGAAAAACAGGCAGGAGGGTGTCAATC10.37593984962406013No Hit
GAGTGTAGATCTCGGCGCCGTATACCCATGTACTCTGCGTTGATACCACT10.37593984962406013Clontech SMART CDS Primer II A (95% over 22bp)
ATCTCTACTAAAAATACAAAAATTAGCCAAGCGTGGTGTCGCACGCCTGT10.37593984962406013No Hit
ACTCTTCCCTCACGACGCTCTTCCGATCTCATGATGCAGCAACTCCAGTC10.37593984962406013No Hit
AAACTGCTTTGCCCTTTCCTCTCACTGCCTTTTATAGCCAATATAAATGT10.37593984962406013No Hit
ATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAGT10.37593984962406013Clontech Universal Primer Mix Long (95% over 21bp)
ATGCAAATAATGATCAACCTGAGGACTTGATTCAGGGATGTTCAGAAAGT10.37593984962406013No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
ATTTTTTTGATGTCATTTTGTGTAAGGGCGCAGACTGCTGCGAACAGAGT10.37593984962406013No Hit
AAACAAAAATATTTACCAACACGACAAAAAACCGATATATCCCAATATTT10.37593984962406013No Hit
GAACTGGCTGAACCGCCACAGCTGGCAGCGGACCCCTGTTGCCGTCACCG10.37593984962406013No Hit
ATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCG10.37593984962406013No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT10.37593984962406013No Hit
CACCCGCGCCAAGGTGTTCTCGGCGATCGGCAAGCAGACCGAGTTGTTCG10.37593984962406013No Hit
CTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC10.37593984962406013No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.37593984962406013Clontech Universal Primer Mix Long (95% over 21bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGTCTGTCTCTTATACACATCT10.37593984962406013Illumina Single End PCR Primer 1 (96% over 29bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAATGGG10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
GGCCATGAGAGGGTTTGAATAGAGGAGAAAAATTATCTGATATGCTAAAA10.37593984962406013No Hit
TCATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTTA10.37593984962406013No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTACCACCAAA10.37593984962406013No Hit
ATCAACGCAGAGTACATGGGGCCACCGAGATCTACACTCTTTCCTACACG10.37593984962406013Illumina Single End PCR Primer 1 (96% over 25bp)
CTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAG10.37593984962406013No Hit
CGTATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTTGATCTGTCTC10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTAAAAAA10.37593984962406013No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC10.37593984962406013Clontech SMART CDS Primer II A (96% over 26bp)
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCACT10.37593984962406013Illumina Single End PCR Primer 1 (100% over 24bp)
ATCCCTTTTTTGGTACCAACAGGCCCATGTACTCTGCGTTGATACCAGTC10.37593984962406013Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CCTGAATGTTTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.37593984962406013No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.37593984962406013No Hit
CACGAGCTGCAGGCGCCCGACGAGCTCGGCCAGGGTCTGCAGTGATTGTT10.37593984962406013No Hit
CAAGATTACATGTATCTAAGCACCATAAATAAAAATAAACAAAACTACGA10.37593984962406013No Hit
GTACTTTTTTTTTTTTTTTTGGTTGATTGTAGATATTGGGCTGTTAATTG10.37593984962406013No Hit
TCTACAAACATTACCTAAAAAACGCTCTAACTAAAACACAACACCGAGCC10.37593984962406013No Hit
GCCTCCCTCCACGCTCTTGGCCTGGTTGACCTCACCTAAACCCTCCAAAA10.37593984962406013No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAA10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGTACATGG10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
CGAACGGCCCGCTCAACATGCGCGCGGCGACCTTCATCGGCCCGAACTAC10.37593984962406013No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG10.37593984962406013No Hit
CATGGCAAAGTGTGCTAGGAACTGGGAAACACTACACAAAAGTAAGGATT10.37593984962406013No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCTGGTCCGTATCGCGTA10.37593984962406013No Hit
CAGCTCGACCGGCTGCTGGACCAGCAGAGCGAGCGGCTCCGCGTCGTCGA10.37593984962406013No Hit
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.37593984962406013Illumina Single End PCR Primer 1 (100% over 25bp)
CCTGCACTTTCCCCGTGGCCCAGAACCTTGTGGCACCTCCAGAGGGACTG10.37593984962406013No Hit
CCTGGGACAGATCGGAAGAGCGTCGTGTAGGGACTGTCTCTTATACACAT10.37593984962406013Illumina Single End PCR Primer 1 (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
GAGTTAGTCCGGTGGCCGTTATCCAAAAAAAAAAAAAAAAAAAAAAAAAA10.37593984962406013No Hit
TCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGATACCAGAA10.37593984962406013Illumina Single End PCR Primer 1 (100% over 25bp)
CAAGCAGAGGTATCAACGCAGAGTACATGTGAATCAGTGGTATCAAAGCA10.37593984962406013Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGTAGGGAAAGAGTGTAGACTCGGGCGATCCCATGTACTCTGCGTTGAT10.37593984962406013No Hit
TCTCGGTGGTCGCCGTATCTTCCCATGTACTCTGCGTTGATACCACTGCT10.37593984962406013Clontech SMART CDS Primer II A (96% over 25bp)
CTCTGGATTCTATTGAAGGCTTGTAACATTCAGGGGAAGGCTTGACAGTA10.37593984962406013No Hit
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA10.37593984962406013Clontech Universal Primer Mix Long (95% over 21bp)
CTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTCCATTTTC10.37593984962406013No Hit
GAAGTATAGCTTTACAGATGAGGAGAAGGAGCTCTTTAGAGGTCGGAGTC10.37593984962406013No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCA10.37593984962406013No Hit
GAGATGCTGCGGCCCGCGGCCCTGCTCCGCGGCCAGGAGTGGTTCCGGGC10.37593984962406013No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
TTTGATTGATGTTCGACATGCAAACTCGATCAAATGTGGGAGCCGGGCTT10.37593984962406013No Hit
TCCCAGAAGTGATGGCTAAGGGGAGGGAGGAGGGTGAGAAGAGGAAGACA10.37593984962406013No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAGTCTGCGTTGAAACCACAG10.37593984962406013No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGAGTGGTATCAACGCAG10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTTTCGCTATTGTCACCCAGGCTGGAGTGCAATGGCACAATCTTGGCT10.37593984962406013No Hit
CTCGGTGGTTCACCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT10.37593984962406013No Hit
ACCGAGATCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGT10.37593984962406013Illumina Single End PCR Primer 1 (97% over 38bp)
GTACATGGGTGACGGGACCACCGAGATACACTCTTTCCTACACGACGCTC10.37593984962406013No Hit
GTACATGGGTGAGCACCACCGAGATCTACACTCTTTCCCAGGTACCTGTC10.37593984962406013Illumina Single End PCR Primer 1 (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGTGTACGGCGACCACCGAGATTAC10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGCCGAAGCCACCGTCAAGGGCACGCTCACCGGCACCGAAGCCCGCCTGC10.37593984962406013No Hit
CTTTCCCTACCGACGCTCTTCCGATCTATCCCTTTTTTGGGCCGGTTGGT10.37593984962406013No Hit
GAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAGTA10.37593984962406013Clontech Universal Primer Mix Long (95% over 22bp)
AAAAACTCACACTAAAACACATAACCGAGACCACCAAAATACAATCTACC10.37593984962406013No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAA10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 22bp)
ATTGTGGTGAGAGGTGAGAATAGGGAGGTGAGTGAGAGATAGCCCTATCC10.37593984962406013No Hit
AAAAAACTAATAGTACAACAGTGACATCGCAAGAGATCTAAAATATACAA10.37593984962406013No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.37593984962406013Clontech SMART CDS Primer II A (96% over 26bp)
ACATGGTTTACCAGATTAATCTATAAATTCAATACAAATCCAATCAAAAT10.37593984962406013No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCATAGTACTGGGTGAT10.37593984962406013Clontech SMART CDS Primer II A (96% over 26bp)
GATCGGAAGAGCGTCGTGTAAGATGTAATCTCGGTGGTCGCCGTACTTCC10.37593984962406013Illumina Single End PCR Primer 1 (95% over 22bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.37593984962406013Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC10.37593984962406013No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCCTGACTTGAC10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTG10.37593984962406013No Hit
CAACAAGACGCTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGGGG10.37593984962406013No Hit
GTGTGACATCGATGTTCCGTTCCTCTTCACGGTGACCGGTGGTACGGCCC10.37593984962406013No Hit
TCTACACTCTTTCCCTACACGACGCTCTGTCCCAAGTTCTGCAGGTACCA10.37593984962406013Illumina Single End PCR Primer 1 (96% over 28bp)
GGGCAATAAAGCAAGACTCCATCTCAGAAAAAAAAGAAAAAAAAATAGAA10.37593984962406013No Hit
GTACATGGTCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT10.37593984962406013Illumina Single End PCR Primer 1 (97% over 43bp)
GAGGTTAAGCCTTCTTTTGGGATTTGCATTGTAATTGAGGGCCCTGGAAG10.37593984962406013No Hit
TCTTAGGTGTGGTCTGCTGTGAAAGTCCACTGCAAGATGATGTCCCCTCT10.37593984962406013No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTGACATCGATGTTCCGTTCCTCTTCACGGTGACCGGTGGTACGGCCA10.37593984962406013No Hit
ATAATATTGAAAAAGGAAGAATATGAGCCATATTCAACGGGAAACGTCGA10.37593984962406013No Hit
ACTGTGGAGAGCGCTCATCTTGCTGCCAGCACCCCTGTCACTGCAGCTAG10.37593984962406013No Hit
ATCCGCGACGGCTTCGACGCGGCGGCGGCCATGCACCAGTGGTTCGAACA10.37593984962406013No Hit
TAATCAGGATGCCATCCTTTAGGTTCTGGGGAAATATTGAGTCTAGCCCT10.37593984962406013No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGATGCGTTTATA10.37593984962406013No Hit
GAGCCGAGACTGCGCCACTGCACTCCAGCCTAGGTGTCAGAGTGAGACTC10.37593984962406013No Hit
ATTCAGCGTGCCGGAGGGGGATTCGCCCACTCTTATAACTTCCCCTCTTT10.37593984962406013No Hit
AGATGAACTCTTTCATTCCACCTGAGCTGTCAGGAAACTGTCTCTTATAC10.37593984962406013No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC10.37593984962406013No Hit
GAGTACATGGGTAGCCCCGAGATCTACACTCTTTCCCTACACGGAGCTGC10.37593984962406013Illumina Single End PCR Primer 1 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTAACACTGCTT10.37593984962406013No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATGACCTTACA10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCCAAGCAATAGAAATCATTTTAAAGTCTTTCAGATCCTAGGCACTAA10.37593984962406013No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGCCAC10.37593984962406013Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)