FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33.1914893617021276Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGCAGCCCGCTGGCGTGGCGGCGGCGTCACCGCACGCAGCCGAGCGGTC22.127659574468085No Hit
ACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAG22.127659574468085Illumina Single End PCR Primer 1 (100% over 43bp)
GCCCGGCCTTGAGGTCAGGGGCAGTGGTGCCGGAAGAGGTCGCGGGGGAG22.127659574468085No Hit
CCATGAACCCGACTCTCATGAACCCGCTCGCGCCGCTGCGCCTCACGAGT22.127659574468085No Hit
CCCTATGGTGAGATTCTTCGCCGATCCATGCTGTTAGGGTCGATTCTGGA22.127659574468085No Hit
TTCTACAAGGACCTGCGCAGCGACCCCGCCACGATCCGCCACGCGTTCGC22.127659574468085No Hit
GCTTCCTTCCTTTTGGAATCGTGCTCTTGGGAATAGCTATTGTTTGCTGC11.0638297872340425No Hit
GTCCAGCCCTATGTCGGAGCCCTCATGCTCCCGCATCACGGCTCTCATCT11.0638297872340425No Hit
CCCCTCGACAATCGCCACCAGCGGACGTCCGGGATCGGTCCATTTTCCGG11.0638297872340425No Hit
GGCCTGCAGCAGGTCGAAGGCCTCGGGTCCGCGCACCTCACCGACGATGA11.0638297872340425No Hit
ACTGATCCTTACATTCAAAAGCAACAGACGCGATCGCTCCTCTGTCTACC11.0638297872340425No Hit
GTACATGGGAATGATACGGCGACCACCGAGATCTACACTAGAACGGGGGG11.0638297872340425Illumina Single End PCR Primer 1 (96% over 30bp)
ACCGGCACACGACCGCCCTCTCAGGGGAGATTCGAGTTCGCTTGGCGAAT11.0638297872340425No Hit
GCATCTGACTCATGTCGTGCGGAGCGGCAACGGTGGTTCTGGAATGCCGT11.0638297872340425No Hit
GTGCACCACGCCGCCGGCATCCCGCCGCACAAGGTCAGCTGGCGCGAGAT11.0638297872340425No Hit
GGGTGCATGTGGGTTCCAATCACGCGCGTGATCGAACCGGACAAGGCTCC11.0638297872340425No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAA11.0638297872340425Clontech Universal Primer Mix Long (95% over 24bp)
CATCATCACAGCACGATAGCGTGACGACGATTCATGCAGCTTTATAAACA11.0638297872340425No Hit
GGGTCGCGCTTGCCGCGTGTGAGCGAGCGGCCCGGGCCGTCACCGCGCAG11.0638297872340425No Hit
GCCTGAAAGACCCGGAACGCAGTGTTGGCAAAGAAATCGGTAACCTCGGT11.0638297872340425No Hit
CCAATGTGCGGGTCGACCCGCTCCACGATCTCGCCGTCCAGCTCCAGCAC11.0638297872340425No Hit
GCCTGCGCCAACCTTCAACCGACGAGGGCCAGGCACTGTCTTCACGAACA11.0638297872340425No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.0638297872340425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.0638297872340425Clontech Universal Primer Mix Long (96% over 26bp)
GGGTGGCGGTGGGGCCCTCCGCGCAGTTGAGGCAGCGGGCCAGGATGCGG11.0638297872340425No Hit
TCCCCGTTTCGAGCAAGGCCAAGCAGATGGGCGGCTCGACGATGTTCCTG11.0638297872340425No Hit
GGGGCGGCGACCGGCCCGTCCGTCCCGGCGGTGGCCCGCGCCACGAGCAC11.0638297872340425No Hit
GTACATGGGTGTACTGCGACCACCGAGATCTAAACTCTTTCCCTACACGA11.0638297872340425Illumina Single End PCR Primer 1 (97% over 35bp)
TCCTCGGTCTTGACGCTCTCGAACAGCGTAGCGTCGTCGAACGCCTCCTG11.0638297872340425No Hit
GTGCTGGACGGAGTGCGCCCGTGCCAATGCCTCCCCTGAGATCAAGGTGT11.0638297872340425No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.0638297872340425Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGATCACCCATG11.0638297872340425Illumina Single End PCR Primer 1 (97% over 40bp)
GAGCGTCGTGTAGGGAAAAGTGTAATCTCGGTGGCGCCGCCCATGTACTC11.0638297872340425No Hit
GGTTCAAGGAGAGGGGTGGACCGGGGCGGTCGCGCTCCCTACCTTTCGTG11.0638297872340425No Hit
GTGGGTGCCCCGGTGCCCGCCGGCCGCCGGGCCCTGGACCGTCAGCAGGT11.0638297872340425No Hit
ACCAGCTACTCAGGGGTACCCCTCCCTGAGGATGGAGGGATGCACACCAA11.0638297872340425No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.0638297872340425Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCGAGCGATCCGGTCAGGCGCCGGAACGCCGCCGAGGGGTGGGTGCC11.0638297872340425No Hit
AAGCAAACTACATAACTAAGCACAGCTATCAAATAAAAAAAAATCCGAAC11.0638297872340425No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.0638297872340425Clontech Universal Primer Mix Long (96% over 26bp)
GTTCGCCGAGCGCGCCTACGGCCTCGACGGCCTGGAGCAGAAGTACGGCT11.0638297872340425No Hit
GTCCACGGCCTCCTTGCCGCCGTGCAGCACCTGGAACACCCCGTCCGGCA11.0638297872340425No Hit
GTTCTGGATCGCGCGGTCGCTGGCGCCGGAAAGCCGGCTTGGCGCGGCCG11.0638297872340425No Hit
GGCCTCGAAGGTGCTCGGCCGCCGGGCCGAGCAGGCCCCGCAGGACTGAT11.0638297872340425No Hit
AAATTGTCTAGTGAGTACACATAAACCTTCCTTCGGAGGTGCGGTTTATG11.0638297872340425No Hit
GTCTGGAGCGACGCCTGCGGTGACGTTGTAAGTCAGCAGCGACACGGGAG11.0638297872340425No Hit
GCCGGGGACCCTGTCGGGCCCCCGGCCGGTCACGGCCGTTCGGCTCAGGG11.0638297872340425No Hit
ATCCCGTAGTGGGCGGTGAAGGCGGTGGCGGAGACGATGGCCTGCCCGGG11.0638297872340425No Hit
ATTTCAGGCTGTGAATTCTGGAACCCCAGAAAACTTCTTCTAAACATCTC11.0638297872340425No Hit
GTGCCGATGTGGTTCCTGCTGGACACCGCGTCCCTGCCGCTGTTCGCCCT11.0638297872340425No Hit
AGACAATGGCGGCCAGTACCAGCACTGCCAAGGCCAGAGCAGACGCTGTG11.0638297872340425No Hit
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG11.0638297872340425Clontech Universal Primer Mix Long (96% over 26bp)
CAGGAGAGAAGTGGCCAAGCCCCGCGTTCACGAGCTCGCGAAAGAGCTCG11.0638297872340425No Hit
CCACCCGCCGGGTGGTCCGGTACAGCAGCCGCACGCCGAGCTGCTCCTCG11.0638297872340425No Hit
GCCAGGGAGTCGAGCTCGTCCGCGGAGGGCCGGTACAGGCCGATCCAGAC11.0638297872340425No Hit
GGTACAGGGGCTACCCGCTGGCGGGCACGCTCGTCACGGCCTCGGCCATG11.0638297872340425No Hit
ACTCTTCCCTACACGACGCTCTTCCGATCTCCCTGCTGGTACCAGTAGAC11.0638297872340425Illumina Single End PCR Primer 1 (100% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.0638297872340425Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCCACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0638297872340425No Hit
GTACATGGGGATACGGCGACCACCGGGCTGGACACTGTTTTTTTTCGTCG11.0638297872340425No Hit
GGCCATGGGCTGGGGCGCCGGGGCCGGTGCCAGGCTGAGCTTCAGAAGGG11.0638297872340425No Hit
GGCCAATTACACCCAAACAGAAGCTTATTTTAAAAAGCTAGCAGAGACTT11.0638297872340425No Hit
CGGCGCTGTAGTGCCAGCCTGTGCCCCAGTAGCCGTGGTTCGGCGAGAGC11.0638297872340425No Hit
TGTCCAGACAGGGGCCCAGGGACACGCACCCCGAATGGGCCCTCAGGAGA11.0638297872340425No Hit
ACGCTGCTGGTGCTGCGCACGCCGACCGCGGTCGACGCGCCGGCGCGCTA11.0638297872340425No Hit
GTTCTACTCGACGCCGCGCGGCCTGAGGATGCGCCGATGAGCGCGGACCC11.0638297872340425No Hit
CATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.0638297872340425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGGCAGGCGAGGTCGTCCACCCGGTGGCCGGGGCCCACAGCGTCGACGTC11.0638297872340425No Hit
CATGTAGGTCGAATTGTCCAGATCATCGATGATCGGCAGGTTGCGGGTGC11.0638297872340425No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGTACGGC11.0638297872340425Clontech Universal Primer Mix Long (96% over 26bp)
CGTGTGGCGCCACACCCGCCGCGGCGACAAGTACGTCACCGTCATCATCG11.0638297872340425No Hit
GTGCTGTGACGCTGCGATCAAGCCAGATGTCGAACTGGATGTGATCAGGG11.0638297872340425No Hit
GGAAGCAGTGGTATCAACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0638297872340425No Hit
GGCGCGGGTGATGATGCGCCCGGTGGGGCCGTCGTGGATGTCGTGGTCGA11.0638297872340425No Hit
CTTCTAATAGTTCCCTTTAAAAAAAATCTAAAATCACCCCAAATTTCTGC11.0638297872340425No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA11.0638297872340425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTGGGGCGGCTTCATCGCCGCCGTGCTCCACCTGCGGCGCACCCCCGA11.0638297872340425No Hit
CCGTCTGGACGGGAGCCGTCAGCCCCGGTGAGCCGTCAGCTCCAGGCGCG11.0638297872340425No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.0638297872340425Clontech Universal Primer Mix Long (95% over 21bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCTGCGTTGATACA11.0638297872340425No Hit
GTGCAGGTGCGGAACTCCAAGGGGCAGGTCCTCAAGACTTTTTACCCTCG11.0638297872340425No Hit
GTCCAGGCGTGAAACGACGCCAACGGCGACAGCCCCATCACCGCCGAGTA11.0638297872340425No Hit
TTTCATATTTTCTTCTACTGTTAAGCCACCAAAAATCCCTTGATCTTCAG11.0638297872340425No Hit
GGTTTGATCCGCGTCCAGGCGGTCGGAGGCACTGGCGATCAGCAGGTTGC11.0638297872340425No Hit
GTTGAAGGATCGCCATGACTGAACAGGTTCAGGAACTGCCGGCGGTCGTG11.0638297872340425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)