FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94
Sequences flagged as poor quality0
Sequence length100
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG66.382978723404255Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGTAGATGGGCTCGACCGAATCGATGGTATGGCCGCCCGCAATGGGGA22.127659574468085No Hit
GTTGCCGTACGCCTTATCCGCGTGCAGCTTCCTCGGGCGGCGTCGTGGAC22.127659574468085No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT22.127659574468085Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCGGCACCACCTCGTTGCGCCTGGACCCGGCGCCGCCCGGCGTCACCG22.127659574468085No Hit
GGGTCCATCACCGCGACCGCCTCGGGCAGCTCCTCGGCGGTGGCGGTCTT11.0638297872340425No Hit
AGCCTGGGGTGTCACAATCTACAAAGTCGGCGACCCAGTCCTCTTTAACG11.0638297872340425No Hit
GTCCAGGAGCTGCGGGTCCGCGCCGAGCGCCCGCTGATCCCGCCGGAGCA11.0638297872340425No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG11.0638297872340425Clontech Universal Primer Mix Long (96% over 26bp)
AGATTGCTCTGTTCCGCGGGTTCAGCGTCAGCCCGGCGATCCGATGGGAG11.0638297872340425No Hit
GAGTACATGGGTGTACGCACCCCTCTACACTCTTTCCTACACGACGCTCT11.0638297872340425No Hit
GTGGTATCAACGCAGAGTACATGGGAATGATACGGCGACCATCGAGATCT11.0638297872340425Illumina Single End PCR Primer 1 (96% over 25bp)
TAAGATAAAAGGAAGGATGGTAGTCCCATTGTGTCTGAAAAATATGTCAA11.0638297872340425No Hit
CTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGAAG11.0638297872340425Illumina Single End PCR Primer 1 (95% over 22bp)
GTCCAGCATCTCCTCCCACGTGGTGGGGACCTCGACGCCGGCCTCCTCGA11.0638297872340425No Hit
GCCCTCACCAGTACGATCCACCCTTCCATCTCGCGACCGGCACGCCCCGC11.0638297872340425No Hit
GCGCTGCGGGTCCCGGTCGTCTCGTTCACCTTCGGGCTCCCCGCGGTCTC11.0638297872340425No Hit
CTGGTGATCTCGGGCGGCACCGGCTCGGGCAAGACCACCCTGCTGAACAC11.0638297872340425No Hit
GCGACTCGACCGCCGGCAGCTGGCGAACCGGCAGCTCGACGACCTGGCCG11.0638297872340425No Hit
GTTCCTGCTGGCCGTGCTCGTGTTCGGCGAGCAGATGCCCCTGGAGCGCT11.0638297872340425No Hit
GTGGGCTCGGAGATGTGTCTTCTGCTTGCCCATGTACTCAGCGTTGATAC11.0638297872340425No Hit
GTGGTTCAACGCAGAGTACATGGGTGATCGGCGACCACCCAGAAATACAA11.0638297872340425No Hit
CGAGAACGGCGAGGAGATCGACCACTCCGCGGCGAGGTCGATCTCCTCGC11.0638297872340425No Hit
GTCCTCGTGCACCTCCTCGACGTGCAGGTCGATCTCCTGGATGTCCTTCG11.0638297872340425No Hit
GCCCAGAGAGAAGCCGGCGAGGACCGTTGCGATGACGGCCGTCCAGGTCA11.0638297872340425No Hit
GCTTCGAGGGGCATCCGCTGCGCAAGGATTTCCCCATGACCGGCTATGTC11.0638297872340425No Hit
GTCACGGGCTCGGGATAGTGCGCCTCGCCAAGGTATTCGATGGCGATGCT11.0638297872340425No Hit
GATTAATTGATGAGCTCCTACAACTTCGTTTGCATGTAAACCGCCGTCTA11.0638297872340425No Hit
ATCGCTGCCTGAGCCATCAACAGTTTCAGGACTTGTAAGCGATCGCTAGT11.0638297872340425No Hit
CCCCTAGGACCGGAAGGTAGCAAGGGTAAATGGGCTCTGGCGGGTGCGCG11.0638297872340425No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.0638297872340425Clontech Universal Primer Mix Long (96% over 26bp)
TCACCGCACCGGTGCACTACCTGTTCCGCCCCTACATCGTCTACCGCTCC11.0638297872340425No Hit
CCTACGGACCGCGCTGCCCGGTTGCGCCGTCCGTACTTGAGCAGCTCAAC11.0638297872340425No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.0638297872340425Clontech Universal Primer Mix Long (96% over 26bp)
CCCCAGCAGAGCGCGGCCCGCGCTGACCTTCACGTGCGTCCACAGCTCGC11.0638297872340425No Hit
TCGGGCTGCAGGCCGCCGTCGCGGTGCAGTTCCACGAGGTGGCCTTCGCC11.0638297872340425No Hit
TCGCCGCACCGGTGCACTACCTGTTCCGCCCCTACATCGTCTACCGCTCC11.0638297872340425No Hit
TGCCTGGCCAGAACACGTCTATGAAGCCGGTGCCCTTGGAGATTCGCTTG11.0638297872340425No Hit
CACGAGATCTACACTCTTCCCTACACGACGCTCTTGCCTCAGATTCTGTC11.0638297872340425No Hit
GCTGCAGCGCGGCGGGCGTCGCGCCGAGCGTGTCGATGGCGTCACGCAGC11.0638297872340425No Hit
GCTACACCGCCACCCGCCACCAGCGCGAGGTCGGCACCGGCTACTTCGAC11.0638297872340425No Hit
GTCCTGAGCCATGGCTGGCGCGAGCGCCCCCGAAGCAACCAGCAGAGCGC11.0638297872340425No Hit
AGCGTCGCCTCGGTCGTGGGCCTGCGCACGGGGCCCGGCGCCACGGCCGT11.0638297872340425No Hit
GTACATGGGCCTGACTTGACTTCTCCACCCAGATCTACATCTTTCCCTAC11.0638297872340425No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.0638297872340425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCCGGGCCGAGCAGATCGCCGTCGCCTGCTTCGCGTCCCTGGCCCAGG11.0638297872340425No Hit
CATCGAAATCGAAGGTGTCGAGGGCGAAGTACTTGTAGTATTCGGGAAAA11.0638297872340425No Hit
GGTCCTGAAAAACGAGCGTCGTGTAGGGAAAGAGTGTAGATCGGTGGTCG11.0638297872340425Illumina Single End PCR Primer 1 (96% over 29bp)
ACGTGGGACTGCTCATGGTGGTCGATCCTGAGCTCGCCCTGCGTCAGGCA11.0638297872340425No Hit
CTACCGGGGCGGGCAGTGCATCGCGTCCCCCGCCACGCTCGAGGAGACCT11.0638297872340425No Hit
GTCTCGCTGCTCGTGAAGATCGCCCCGGACCTGTCGGACGAGGACGTGCT11.0638297872340425No Hit
AGTTCGCCTGCGGGATCGCCGAGCTGTGGCAGCGGGCCGGACTGCCGGAC11.0638297872340425No Hit
GGCGGCGAACACCTCCGGCCCGTCCACCGTCAGCCGCCCGTCGGAGACGG11.0638297872340425No Hit
GCCCCGGGGTGCACGGCGGCAGCGGAGTCGATTAGAGGTCACCGATGTCC11.0638297872340425No Hit
CCGACACCGTCGTTGGCAGTTTCCCGTTTCAGGCCGCCAAAGTACCAGGC11.0638297872340425No Hit
GACTACGGGCACGCGGCCGCGGTGGCCCTGCCGGAGGAGCGCGGACGGAT11.0638297872340425No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCACTGTCTACTGGTACCAGC11.0638297872340425Illumina Single End PCR Primer 1 (100% over 30bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT11.0638297872340425Clontech Universal Primer Mix Long (96% over 25bp)
TAATGGTGCAGGGAAAACGACAACCCTACGTTCAATTATGGGTTTAAATC11.0638297872340425No Hit
TCCAGGCGGCACGCCGTTTTGCCACCACGCAGATCGACAACTACAACCGC11.0638297872340425No Hit
CCGTTACCTCGCATCACCTGGCACGAGGCGATGGATCGCTACGGCTCTGA11.0638297872340425No Hit
CGCTGACAAGGTGTGATGTTATCGTGCGCTGACATCAACTATTGTCGTCG11.0638297872340425No Hit
TCACCCTTCTAAAGGTAGGTCCTGCCCGTTCACGAGTGTTTCCCAGAACG11.0638297872340425No Hit
ATGCAGGAGGCAGAGGCAGATGGATCTCTGTGAGTTAGAGACCAACCTGG11.0638297872340425No Hit
GCCTCGAGCTGGGCCTGGAGAACCGGCTGGGCGCCAAGGTGGGCCTGATC11.0638297872340425No Hit
GCGGTAGACGGTGTCACCCTCGGGCACGGCCGCCCCCGGTGCTCGGGTCC11.0638297872340425No Hit
GCATCCATTTCTGCGAGACGTGAGGGTCCTTGGTCCGGAAGCGCAGGCAA11.0638297872340425No Hit
TCCGGTCAGGGCGGCAGCGGCAAGACCTGCGGATGCGTGAAGGAAGTGCC11.0638297872340425No Hit
TCCCGGCCCTGGCCCCTGAGCACCGTGGCGCGGCGATCTCCGCCCACAAC11.0638297872340425No Hit
GGGGCCACCCCGTGCTCGGGGCAGCAGTGCCAGAAGCACCCGTCGACGAA11.0638297872340425No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGGACGACAGAGATCTACAC11.0638297872340425Clontech Universal Primer Mix Long (96% over 25bp)
AGGTTGTTGGTGAAGATCGCCCCGAAGATCGCCACGCCGAGGGCCGCGCC11.0638297872340425No Hit
GCCGGCGAGGGCGGAGCGCAGCTTCTTGGCGACGGGCGGCTCGACGGTCG11.0638297872340425No Hit
ATGCACACCTACGAGGTGTCCGTGACCGAGAAGGATGCTACCTTCTATAT11.0638297872340425No Hit
GTTTGGACACTATGTTCTTTCACAAAAGGGATTTCATTTTGTGAAATATT11.0638297872340425No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT11.0638297872340425Clontech SMART CDS Primer II A (100% over 26bp)
TGGGAAGATACGGCGACCACCGAGCTCTAAACACTTTACCAACACGACGC11.0638297872340425Illumina Single End PCR Primer 1 (95% over 23bp)
CCTCGGCAACCGTGGTGATTTACGGAAATTGTAATCGTCGCGTAGGCCAT11.0638297872340425No Hit
CTACGGGAGGAATCATGGGATTTGAGGTGTTCGACAAGAGGATGACTCCG11.0638297872340425No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTAAAAAAAATAAATAAAAAAAATAA11.0638297872340425No Hit
CCTTGGCACAATGGGAGCACTATGCCTACCACCCCTGAGACGGCGAGCAC11.0638297872340425No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGGTTTAAAACCAA11.0638297872340425No Hit
GTCCGCCGCGGCCAGCGGCAGGACCTGCTGGGCGATGAAGTTGTTCTCCG11.0638297872340425No Hit
CGAGAACGGCGAGGAGATCGACCTCTCCGCGGCGAGGTCGATCTCCTCGC11.0638297872340425No Hit
GCTCCTGTCGGCTCATGGCCTTCAGGGCTTCGGGAGAGCCGCCCACGAAG11.0638297872340425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)