FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT44.819277108433735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33.614457831325301Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCTGAGGCAAGAGCGTCGTGTAGGAAAGAGTGAGATCTCGTGGTCGCCG33.614457831325301No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33.614457831325301Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT22.4096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.4096385542168677Clontech Universal Primer Mix Long (96% over 26bp)
TTTACAAGGCTGGGCCATATAAAAGCATTGCAAACATGGAGTGGAGAGGA11.2048192771084338No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
ACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGACAGAGATC11.2048192771084338No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAATCAGCCGAGATACCAA11.2048192771084338No Hit
TATCAACGCAGAGTACATGGATGATAGGCGACCACGAGAATACCTCTTTC11.2048192771084338No Hit
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA11.2048192771084338Illumina Single End PCR Primer 1 (100% over 21bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGTAGGCGACCACGAGATCTACACTCCCTGTCTCTTATACA11.2048192771084338No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.2048192771084338Clontech Universal Primer Mix Long (100% over 23bp)
GTGCTCCCATGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACAT11.2048192771084338Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATTATGAATAATACTGCTGTGAACATTTGTTTACAAGTTTTTGTGTGAAC11.2048192771084338No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACAT11.2048192771084338No Hit
TCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTCTGTCTCTTATACA11.2048192771084338No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCTC11.2048192771084338No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGATAC11.2048192771084338Illumina Single End PCR Primer 1 (100% over 30bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACTGCGGTGATAAAA11.2048192771084338No Hit
CCACTGATGAGTACCTAGGCTGATTATATGTCTTTACTGTTGTGACTAGT11.2048192771084338No Hit
GAGTGTAGATCTCGGTGGTCGCCGTACACCCATGTACTCCTGTCTCTTAT11.2048192771084338Illumina Single End PCR Primer 1 (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTGTTACCACTGCTTCCCTG11.2048192771084338No Hit
GTACATGGGGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTC11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGT11.2048192771084338Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTCCGATCTCTGGTACCTGTCCCAAGTCTGCAGGTACCAGAGATCGG11.2048192771084338No Hit
ACATGGGTTACGCACCACCGATCTACACTCTGCGTTGATACCACTGCTTC11.2048192771084338Clontech SMART CDS Primer II A (95% over 24bp)
AAAAAAGCAAAGAAAATAGTTGAACGAAACAGAGAACAACACTCTATCAC11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGT11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACACTCATCTGTGGACAGTGAATAATGCTTTGGTCACCTGGTCAGGAA11.2048192771084338No Hit
GTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACCCATGTACTCTG11.2048192771084338Illumina Single End PCR Primer 1 (97% over 39bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAACACTGCTTCCCAT11.2048192771084338No Hit
GGGAAAGAGTGTAGATCTCGGTGGTCGCCGTACATCCCCTGTCTCTTATA11.2048192771084338Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTTAGGAAGATGTCTGCGCCCATGTACTCTGCGTT11.2048192771084338No Hit
GAGTACATGGGAAGCAGTGGTATCAACTTTGCGTTGATACCACTGCTTCC11.2048192771084338Clontech Universal Primer Mix Long (95% over 23bp)
ACACGACGCTTCGATCTCAGTACCCGCATCATGGTCTGAGATCGAAGCGT11.2048192771084338No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAGAGTACTTTT11.2048192771084338Clontech Universal Primer Mix Long (95% over 22bp)
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT11.2048192771084338No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA11.2048192771084338Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGTGATACGGCGCCACCGAGTTACACTCTTCTCTACTGGTACC11.2048192771084338No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC11.2048192771084338Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATACA11.2048192771084338Clontech Universal Primer Mix Long (96% over 25bp)
AGATTACACTTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTC11.2048192771084338Illumina Single End PCR Primer 1 (100% over 27bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTAT11.2048192771084338No Hit
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG11.2048192771084338Illumina Single End PCR Primer 1 (100% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTGATCCTCGGCAGCGTGCTGATGGCGACCACGGCCCAGCTGCTGCT11.2048192771084338No Hit
TCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGATACCAGAA11.2048192771084338Illumina Single End PCR Primer 1 (100% over 25bp)
GCCCTACGACGGGGACACCGCTCCCCCGGACCGGATCTTGGCGCGCGAGC11.2048192771084338No Hit
ATCAACGCAGAGTACATGGGAAGCAGTCGTATCAACGCAGAGTACATTCT11.2048192771084338Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATAAGCCCGAGACTA11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGCATACCTACAAAGACATCCGGCCATGGCTGAAAAATGCAGCAAAAGC11.2048192771084338No Hit
AGCTCGCGCAGTCCGGCTAGCATCGACAGATCGGCCGGGTTGGTGATGTC11.2048192771084338No Hit
CACTCTTTCCCTACACGACGCTCTTCCGATCCTGTCTCTTATACACATCT11.2048192771084338Illumina Single End PCR Primer 1 (96% over 31bp)
TACACGACGCTCTTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGGGG11.2048192771084338No Hit
CTATAAACTCAAAAAAAATGGAAAGAAGTAGAAACAACGCAGAGTACAAG11.2048192771084338No Hit
CACAACAATAAACGACGAAATAACTATCTAATACACATATCCGAGCCCAC11.2048192771084338No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA11.2048192771084338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCTGTCGCCA11.2048192771084338No Hit
GTGTAGGGAAGATGTAGATCTCGGCCCTCACCCATGTACTCTGCGTTGAT11.2048192771084338No Hit
TACATGTACTTCTCCTATTTAAATTTCTTAATAAATTAATAAGTACTAGT11.2048192771084338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.2048192771084338Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGGCAGAGTACTTT11.2048192771084338No Hit
CACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT11.2048192771084338No Hit
CCCTGCATGTTCTCGCCGCTGGGCAAGGAAGGCTACGAGTCGCGCAAGGA11.2048192771084338No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA11.2048192771084338Clontech Universal Primer Mix Long (96% over 26bp)
ATGGTATAATATCTGCTCTACTGAATTTAATACTATAATTGAAAAGTCTT11.2048192771084338No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCAGGAGTGAAATCTCGTAT11.2048192771084338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)