FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG92.9315960912052117Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG92.9315960912052117Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82.6058631921824107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT51.6286644951140066Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41.3029315960912053Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.9771986970684038Clontech Universal Primer Mix Long (100% over 23bp)
GCTTGGGGGTGGGAATACCAGGAGGCCAGGATAATAGTGTTAATGAAGGT30.9771986970684038No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.9771986970684038Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.6514657980456027Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTATCCGGTAATTTGGCACTCCACCTACAGCCAGCTATTCTGTATGA20.6514657980456027No Hit
TCCCTGGAGCCTGGCGGATCGCGAGGTTAACACTAGTTAACCCCTAGATC20.6514657980456027No Hit
ATCAACGCAGAGTACATGGGGTACGGCGCCACCTAGTCACACCTTCCCTA20.6514657980456027No Hit
GTTCAGGAAACCTCTCCACAAAAGTAGAATAGATGTTGTTGAATAAGCAC20.6514657980456027No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.6514657980456027Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGTAGCCGAGGTTCTGGCACATGGAGATGCGGATGGGGTCGCAGCGC20.6514657980456027No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT20.6514657980456027Clontech Universal Primer Mix Long (96% over 26bp)
TTACGGTACTTGGCATAATTGTAATGAATTACTTGTGTAAAGCTCATCTA20.6514657980456027No Hit
GTACAATGTACCCAATTCAGGTAATGGGTACCCTAAAAGCCCTGACTTGC20.6514657980456027No Hit
AGCCTGGCCACCATAGCCATTTTTAGTAGAAAAGCTGTCTCTACTAAAAA20.6514657980456027No Hit
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTTCTGGTATCGACA20.6514657980456027Illumina Single End PCR Primer 1 (100% over 35bp)
GGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACAT20.6514657980456027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATACACATCTCCGA20.6514657980456027Clontech SMARTer II A Oligonucleotide (95% over 22bp)
TTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGCAGAACTTGGGACA20.6514657980456027Illumina Single End PCR Primer 1 (100% over 27bp)
GTATCAACGCAGAGTACATGGGTATCGGACACCGAGACTCACCTCCCTAC10.32573289902280134No Hit
AAACAACAAAACACTCTACATAAATCAAAAAGAAAAGAAATCAGTTGAAG10.32573289902280134No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGATGGCGTC10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTAAATGGGAAGCAGTGGTA10.32573289902280134Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTCGTTGATAAC10.32573289902280134No Hit
GCGTTGATACCACTGCTTCCCATGTACTCCCATGTACTCTGCGTTGATAC10.32573289902280134Clontech Universal Primer Mix Long (95% over 21bp)
CGGTGGTGCCGTACATCCCATGTACTCTGCGTTGATACCACCTGTCTCTT10.32573289902280134Clontech SMART CDS Primer II A (95% over 21bp)
TTCCTATGCTCATTTCACAGGTAAAACTTTTAACAACTCTTTAGCCATTA10.32573289902280134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCC10.32573289902280134No Hit
GGATAACAGAGTGAGACTCTGTCTCAAAAAACAAAACAAAACAAAACAAA10.32573289902280134No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATGAGGGTTTTTAACCA10.32573289902280134No Hit
ACGCTCTTCCGATCTATCCCTTTTTTGGGCCTGTTGGTACCAAAAAAGGG10.32573289902280134No Hit
GCCTCATTGAGCTGCGGTGGGCTCCTCCCAGTTCGAGCTTCCAGACCACT10.32573289902280134No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTATGCGTTGATACCAA10.32573289902280134No Hit
CTGGAGTTCAGACGTGTGCTCTTCCGATCTATTAACAGTGCATATCCCTG10.32573289902280134Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTT10.32573289902280134Illumina Single End PCR Primer 1 (100% over 38bp)
ATCAACGCAGAGTACATGGGAGGCAGTGGTATCAACGCAGAGTACATGGG10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCATAGACAGTTGGCAAGTAAAGTTATCAAGTCTCAGATTCAATAAAGC10.32573289902280134No Hit
GATTCATTAACACTGAACTCACAGCATCACAAACATTTATTTTTTCTTGA10.32573289902280134No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.32573289902280134Illumina Single End PCR Primer 1 (100% over 22bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATAGGCGAGCACCGAG10.32573289902280134Clontech Universal Primer Mix Long (96% over 25bp)
AGTTGTGTTGAATGAAAGTAATGCAGACAGTTCATTGTAAGTTTTCAGGT10.32573289902280134No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATAGCACGAACAC10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGATTACATGGGAAGCAGTGGTATCAACG10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAGGTTTTAA10.32573289902280134No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAACAATGCTTA10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACGGCGCCAC10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGC10.32573289902280134No Hit
AACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACAAGGCGTTTAT10.32573289902280134No Hit
TAAGAACGGTGACATTTGGGCCTCAGTGACCCTGGAGGACCTTGATGCGT10.32573289902280134No Hit
CAGCTACTCAGGAGGCTGAGGCACAAGGGTCACTTGAACCCAGGAGGCGG10.32573289902280134No Hit
CTTCTCGGTGTCCTCAGGTGAACGCCTTCGTGGCTGAACACTGTTAGGGT10.32573289902280134No Hit
GTCGCGGATGATGATCCGGCCGCTTTCCGCCTCACCGCTCGTCCCGAGCG10.32573289902280134No Hit
GTACATGGGGGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134No Hit
TCTCGGTGGTCGCCGTATCATTTCCATGTACTCAGCGTTGATACCACTGC10.32573289902280134Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TGAAGGGGCTTATCCTGTTTGGAGCTTGTTCAGCTTCTTGAATCCTGGTT10.32573289902280134No Hit
GGTATCAACGCAGAGTACATGGGAATCGCACAGTCTACACTCTTCCCTAC10.32573289902280134No Hit
AGTGAGTACGATGGCCAACCGGCTGCAAGTGATCTATATCGAAGACGATG10.32573289902280134No Hit
CCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTG10.32573289902280134No Hit
GTACATGGGAATGATACGGCGACCACCGAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Illumina Single End PCR Primer 1 (95% over 22bp)
CCCCGTCTCTACTAAAAATACAAAAAATTAGCCAGGCGTGGTGGAGGGCG10.32573289902280134No Hit
CTACAACCTTCAGAAAAACGAAAACAACATAATAAAATACACAACTCACA10.32573289902280134No Hit
AGCCTCTCTTGAAGGTAGTTGTGGCAATAAAGTGTAGTCTCCATGAAAGG10.32573289902280134No Hit
GAATGCTGTGTCTGCTTTAGCACGCACGCTCCGAATGACTCCTGGTGCTA10.32573289902280134No Hit
GAAGCAGTGGTATCAACGCAGACTACATGGGCCTTACTTTACTTCTTCTG10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTATCAACGCAGAGTACATGGGCGCACCGAGATCTACACTCTTCCCTAC10.32573289902280134Illumina Single End PCR Primer 1 (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGAGGATTACTTGAACCTGGGAGGTGGAGGTTGTGGTGAGCTGAGATT10.32573289902280134No Hit
GGCCCGGCCTTCGCGATGGTTGGTGGCGCTGATCGCGCTGCTGCTCCTGC10.32573289902280134No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCC10.32573289902280134No Hit
GATCAGCAGGATCAGATGATGCACTGCTGAACGACGGGCCCATTGCGCCA10.32573289902280134No Hit
GGAATACGGCGCCACCGAGATCTACACTCTTTCCCAACACGACGCCCCTT10.32573289902280134Illumina Single End PCR Primer 1 (97% over 34bp)
CTCTTACCATAAAAAAATCTCAAACAATCTATACTCGCGGTCGCCTAATC10.32573289902280134No Hit
TGGTACCACCGAGATCTACACTCTTTCCCTACACACGCTCTTCCGATCTG10.32573289902280134Illumina Single End PCR Primer 1 (96% over 31bp)
CTGTCTGACTACAACATCCAGAAAGAGTCTACCCTGCACCTGGTGCTCCG10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGCTGTCTCTT10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCAGCTCCGAGGGCGGCAGCGAGGCCACCGCCAATGCCTTGCGCTTCG10.32573289902280134No Hit
CTATGATAATCCGCAGCGAAATAATCTACCGACTCTTGAGTACCGACAGC10.32573289902280134No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 22bp)
CAACATCAAAACACTTCACTACAAGAAGAAAATAATAAAAAAATAAGAAA10.32573289902280134No Hit
GGTTTACGGCACCACCGAGATCTACACTCTTTCCCTACACGACGCAGCTC10.32573289902280134Illumina Single End PCR Primer 1 (97% over 36bp)
GGAAAGAGTGTAGATCTCGGTGGTCGCGTATCACCCATGTACTCTGCGTT10.32573289902280134Illumina Single End PCR Primer 1 (96% over 27bp)
ACCGGAAGGACAGCGCCCACGTATGCGGTGGGGTCCACCCTGTCTCTTAT10.32573289902280134No Hit
ATTGATAGTAGGTAAAGTCTATGCAACTCTTCTTACGTGCCTGGACCCGT10.32573289902280134No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA10.32573289902280134No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATGTGCTTTTATACCA10.32573289902280134No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
AAGCTGATGCAGCACAGGCGAGCCCCAAAATTGAGGCTTAGCCCGGGGGG10.32573289902280134No Hit
GCCCACGCCCGACTGGAATTCATAGGCGAACACATCGCCGCCGCCGCGCC10.32573289902280134No Hit
TCGTTGAAGTAGGCGGGCACCGTGATCACGGCTTCGCTGACGGTTTCGCC10.32573289902280134No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGT10.32573289902280134No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGA10.32573289902280134No Hit
GATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTT10.32573289902280134Illumina Single End PCR Primer 1 (100% over 38bp)
CTCTAAAACAAACACTAAAAGACAATATACACATACAATTATACACAACT10.32573289902280134No Hit
GAGTGAGACCGGTCGTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACGGCTTCCCAT10.32573289902280134No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTG10.32573289902280134Clontech SMART CDS Primer II A (96% over 26bp)
GGATACCACCGCGCTGCACATAGCCGAGGATCGAGACTCGCGATTCCAGC10.32573289902280134No Hit
GGGAAAAAACCAATGTATCAGCAGGAGGAGACCCAATCGATGTGACCGAT10.32573289902280134No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 22bp)
GTGCTCATGGATGGTCAGACGACCTAATGAAATAAAAGCGGAAATGTTTT10.32573289902280134No Hit
AGGTGGAACTGGGCCAGCACGTGCAGGCAGGCCAGTTGCTGGCGCGGCTC10.32573289902280134No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.32573289902280134Clontech SMART CDS Primer II A (100% over 23bp)
GCCGGGTACGGCCTTCTGCACCCGCCACAGCGCCGACCGCAGGCTGCCGT10.32573289902280134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTCGTTGATACCACTGCTTCCC10.32573289902280134No Hit
CTATTTGATGACTCTAACACTTTAATAAGGTGCTTAGAGCACAGTGTGAT10.32573289902280134No Hit
GTACATTGGAGATACGACCACCGAGATCTACATCTTTCCCTACACGACGT10.32573289902280134No Hit
CCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAAACGCAGAAAAAAAAAAAAAAAAAAAGTACTCT10.32573289902280134No Hit
ATGATCCACCACAGTGAGGAGTTGATGGTGTCCCAGCACCCATAATCCTC10.32573289902280134No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACACTGCGTTGAT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTTCAATTCTCTAAAAAGTCTTGAACGTCATATAAACAGGACCATAC10.32573289902280134No Hit
GTAAGTGTCTTGGTGAGAGCGCAGATACTACAGAGTTAACCACGAACTGC10.32573289902280134No Hit
GTTCAGCAGCCCGTCCAAAGGGATGCTCCAAGCGCTGTGGGGTGGCGCAG10.32573289902280134No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 22bp)
GGGAAAGAGTGTAGATCTCGGTGGTGTCCCATGTACCTGTCTCTTATACA10.32573289902280134Illumina Single End PCR Primer 1 (96% over 25bp)
ATCCTGGGCGCCACCAGTGAGATCCAGCGGAGAGGACACCAAACCACGTA10.32573289902280134No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTAT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGTCGGCGCCCGGTCACTCT10.32573289902280134Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGTGAAGCAGTGGTATCAACGCAGAGTACATG10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCGATCTGGTACCAACGGGTCCTGAAAAACGAGCGTCGTGTAGGGAA10.32573289902280134No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCCTGTCTCTTA10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCTTTCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGCCTGGTGG10.32573289902280134Illumina Single End PCR Primer 1 (100% over 23bp)
ATTTCAACGTAGTAAAAGCCATATATGAAAAATACAGTGAATGTCATATT10.32573289902280134No Hit
GGACCAAAGTTAATCACTGGAGACAACTGTAGATTCTTATCAGCCCAGAG10.32573289902280134No Hit
AAAATACAAAATTTACCAACACCACTCTCTTAAAAACAAAAACTATACCA10.32573289902280134No Hit
GTGTAGGGAAGAGGTAACTGGTGGTCGCGATCCCATGTACTCTGCGTTGA10.32573289902280134No Hit
AGCCTCTTCTGGCTGGTGATGACCGGCTACACCGCGCGGCTGGCCGTCGG10.32573289902280134No Hit
TCTCACAACAGTGCTCGTTTATGAGGCAACTGTCAGCGATCCGTGCTTTA10.32573289902280134No Hit
ACCTATGAGCAAAAGAACTGGAGACTGAATTCATGGGTGAACCTTTTTTG10.32573289902280134No Hit
GGCCGAGATTGTTGAAAAACTCGATGTGCAGCAACACCAGAATGTGGCGA10.32573289902280134No Hit
GTCTTGTCCGACGCGGGGAAATGGTGCCGACGCTAGTGGTGCAGGCCTTC10.32573289902280134No Hit
AATGGTAGGTGGTGCTGTGTCCAGTTTGGTAGTCTATTTTAGAAAAATGA10.32573289902280134No Hit
CCCTCACAGAGTGAAACTGCTCCAGGCGCCCAGTCATGCCGGGTCACAGA10.32573289902280134No Hit
GTAAAAAACACGTACAAAAACAAACATAATAAAATACACAACTCACATCC10.32573289902280134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTA10.32573289902280134No Hit
GTAGTGTCGGTAGCTTCTGGTGAGGTCTGGGCTGCCCCACGAGGTGCCGC10.32573289902280134No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTTGTAGG10.32573289902280134No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATA10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 21bp)
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAATTACTCTGCGTTGTTAC10.32573289902280134No Hit
GTACATACCGACAACGCTGATGCGCGCGCTCGGAACGTTTTCCAGCACAC10.32573289902280134No Hit
GTAGTAATCATCTCTGTGTGTTTCTGAGCTTGTTGCATTGTGACCTGAAG10.32573289902280134No Hit
ACGCAGAGTACATGGGGACACACCTTCCTACACGACGCTGTCCCAAGTTC10.32573289902280134No Hit
GGTATCAACGCAGAGTACATGGGAAGAACGCCCACGAGATCTACACTCTT10.32573289902280134No Hit
CCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGACGTCGTGTAGAA10.32573289902280134No Hit
GAGTACATGGGATGACGCCAGTTAACTCTTCCCTACACGACGCTCTTCCG10.32573289902280134Illumina Single End PCR Primer 1 (95% over 23bp)
ACGCAGAGCCGCTTCCGCCGGCGCCTCGCCGGCTTCGATCGCGCCCTTGG10.32573289902280134No Hit
TAAGAGACAGGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134No Hit
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAATTAACGGTCTC10.32573289902280134No Hit
GTGCCAGCATACGCAAGAGCCATGGTATACACACCGCCGTAGCGCCTGTC10.32573289902280134No Hit
GCACCGCGGTCCGGCTGGCCGCGCCGGGGGCGCCCCGACCCTGACGGCAG10.32573289902280134No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGT10.32573289902280134Illumina Single End PCR Primer 1 (100% over 36bp)
CAACGCAAAAAAAAAAAAAAAAAAAAGTACACTGCGTTGATACCACTGCT10.32573289902280134Clontech SMART CDS Primer II A (96% over 25bp)
CAGTAGAGTCAGGAAGGGCTGACCAGAGGCTAATGGAATAACCTAAGCCC10.32573289902280134No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134No Hit
CTGAAACATTCATAACAACGCACAGAACAATCTAATAAAAATAACCAACC10.32573289902280134No Hit
ATGTAAATGGTATCTCAATCACGAACCAAGTAGAAAGATGTGCACACCAT10.32573289902280134No Hit
AGCACCCTCTATCCCTAAACTATTACAACAGCTCTTCATTATCTGCGTTC10.32573289902280134No Hit
CCCTACACGACGTCTTCCGATCTCATGATGCAAGGACTGGAGTTGCTGGG10.32573289902280134No Hit
GTAGGGAAAGAGTGTAGATATCGGGCCCATGTACTCTGCGTTGATACCAC10.32573289902280134Illumina Single End PCR Primer 1 (95% over 24bp)
GATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
TTATCAACCAATCAACAAAAATCCTTATTTTACCTGTGTTTTTGTTTGAA10.32573289902280134No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCTC10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAGGCCGGGA10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTA10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCAGAGTACATGGGAAGTAGGGACCACCGAGATCTACACTCTTTCCCTAC10.32573289902280134Illumina Single End PCR Primer 1 (100% over 29bp)
GTACATGGGGATATCAACGCAGAGTACATGGGAACGGCACGTCTCACTTC10.32573289902280134No Hit
AATGAAACAAGTACTATAAAAGCAGCTTACAATTATACACAACTACAACA10.32573289902280134No Hit
ATCTCGTCCCGCTGCAGGCCGGTGGCCAGCTCGATCTCGTCCCAGTCCAG10.32573289902280134No Hit
GTCTTTGTCAGGTATGAAAAAGAAATATACTCAAAATGAAGAGTAAATAT10.32573289902280134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTA10.32573289902280134No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAGGCCGGACCGAGCTGCACAAGGGCGCCGATCCGGCCCGGGACCAG10.32573289902280134No Hit
CATGTACTCTGCGTTGATACCACTGCCCCATGTACTCTGCGTTGCTGTCT10.32573289902280134Clontech SMART CDS Primer II A (95% over 24bp)
GATCGGAAGAGCGTCGTGTAGGAAAGTTAGTCTCGGTGGCCTACCCCATG10.32573289902280134Illumina Single End PCR Primer 1 (96% over 25bp)
ATCTACATCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCT10.32573289902280134Illumina Single End PCR Primer 1 (96% over 30bp)
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTAAATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
AGAATGCGCACTATAGTATAGTTGTCGCAAATTCTAGGTCTGCAAGAAAT10.32573289902280134No Hit
CTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTAC10.32573289902280134Illumina Single End PCR Primer 1 (100% over 28bp)
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 22bp)
CAACATGCAAGCACTGGAATAACAGACGACTAAATCTTATAAACAACACC10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGAGTGTAG10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.32573289902280134Illumina Single End PCR Primer 1 (100% over 25bp)
ACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGACCCATGTACTCTG10.32573289902280134Illumina Single End Adapter 1 (95% over 23bp)
CTGTGAGGGTTGAGCACCGGTTTGCTGGTGCAACCGGCGGCGCTCAGCAG10.32573289902280134No Hit
CTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTAGTTTAAA10.32573289902280134No Hit
GTGTAGACTCGGTGGGCCGTATCACCCATGTACTCTGCGTTGATACCACT10.32573289902280134Clontech SMART CDS Primer II A (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGATACGGCGACC10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAAGCTTA10.32573289902280134No Hit
TTGTGACTCCTAATGGGGATGGAGCTTGGTGTGTGCTTGGACATGGCATT10.32573289902280134No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAGTAAACTTCGT10.32573289902280134Clontech Universal Primer Mix Long (95% over 22bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.32573289902280134Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGACGGGCCAC10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTGGGCCCGGGCTGCCGGGTGCTCGTCTTCTCCGACCTGCACGCCGTCA10.32573289902280134No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATACCTTTTAAACCC10.32573289902280134No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATAACACGGGTTACC10.32573289902280134No Hit
GTTTTTTGTCTGTTTTACCTCAATTTGAACAGATAAGTTTGCCTGCATGC10.32573289902280134No Hit
TATCAACGCAGAGTACATGGGTGACGGCGACCACCGAGACTCTGTCTCTT10.32573289902280134No Hit
ACGCTCTTCCGATCTGTCCCAGGTCTTGTCGATACCAGAACAGATCGGAA10.32573289902280134No Hit
GTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAATTAGAGTGTTCA10.32573289902280134No Hit
GAACCACAGTGACGAAACCGATCGTTCCTAGCGTCTCCGACATGCCTGCA10.32573289902280134No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.32573289902280134Clontech SMART CDS Primer II A (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAGTAAT10.32573289902280134Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
AATCATTCAAATAAACGAATAGAACATAACTCTCACAAAAACACATAAAC10.32573289902280134No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAGTACTTTT10.32573289902280134Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
TGATGGAACAGGCGATCGCTTTGTGTCCTGCTTTATCCCAGGCAACAGTT10.32573289902280134No Hit
CTCGCGGGCATCGCCGGCCGCCTCGGTCAGCTCCTGCGTCGGCAGCCCGC10.32573289902280134No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGTGCGTTTATAC10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGACGTCGTCTACC10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTTGCTGTAGGGAAAGAGTGTAGATCTCGGTGGCTGTCTCTTATACAC10.32573289902280134Illumina Single End PCR Primer 1 (96% over 28bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACCTGTCTCTTATAC10.32573289902280134Clontech SMART CDS Primer II A (95% over 21bp)
GAGTACATGGGAACCGAGATACATCTTCCTACACGACGCTCTTCCGATCT10.32573289902280134Illumina Single End PCR Primer 1 (100% over 23bp)
TCAACGCAGAGTACATGGGAAGCATGGTATCAACGCAGAGTACATGGGAA10.32573289902280134No Hit
GTCTACACTTTTCCCTACACGGAGCTGCCGATACCAGTAAACATGAGATC10.32573289902280134No Hit
ACGCAGAGTACATGGGAATAGCGCCACCGAGTATCAAAATTTACCTAAAG10.32573289902280134No Hit
GCAGAGTACATGGGAAGCAGTGGAATCAACGCAGAGAACAACCCAAACAC10.32573289902280134Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GAAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.32573289902280134No Hit
CCGATCATCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
TGGGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTTTGCCGGGTTGCTGTTGCTCGCACTCGGGCTGCTCGTCCGCGGCG10.32573289902280134No Hit
CAATAAACAAAGACTACATTTGATATAACTCTAACAAATACAAATAACAT10.32573289902280134No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT10.32573289902280134Clontech Universal Primer Mix Long (95% over 21bp)
GTAGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCACCCATGTACCTGT10.32573289902280134Illumina Single End PCR Primer 1 (97% over 35bp)
GAACGGAGATTATGCCGGGCTGGTCAAGCGGCTGCGTCCGGAAACGGCGG10.32573289902280134No Hit
TCATCATCATGACTAACCATTTCCTGCCTCTGTTCATTGCCTGTCTCTTA10.32573289902280134No Hit
GCTCGGACGGCCGGGGCGCATCACCGCGCTGCAGCGTTTCCTCGACCACA10.32573289902280134No Hit
GCAGACGGTTGAGGCCAGGATGGCGCGCCGCGGCCCGTTGCGGTCCACCC10.32573289902280134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10.32573289902280134No Hit
GTGTCGCCGTACTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACAACT10.32573289902280134Illumina Single End PCR Primer 1 (100% over 24bp)
GTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGCCGGGGCGCAGGCCGGGCACCCGCCCGAGGCGCTGCGGGTGGGGCTGC10.32573289902280134No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTTCCCATGTACTCTGCGTTGA10.32573289902280134Clontech Universal Primer Mix Long (96% over 27bp)
CTGATGGCCAGTGATGGTGAGCATTTTTTCATGTGTTTTTTGGCTGCATA10.32573289902280134No Hit
ATCTAAAACAAACAATAAAAGACAATATACACATAAAATTATACACAACA10.32573289902280134No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGGC10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CTACACTTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGAAT10.32573289902280134Illumina Single End PCR Primer 1 (96% over 28bp)
GTGTAGGGAAAAGTAGATCTCGGGGCGCGCTCCCATGTACTCTGCGTCTG10.32573289902280134No Hit
TGTAGGGAAAGAGTGTAGATCTCGTGGTCGCCGTACCCATGTACTCTGCG10.32573289902280134Illumina Single End PCR Primer 1 (95% over 24bp)
TAGACTGGCCGTCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)